Evaluating the Performance of Pathogen-Targeted Positron Emission Tomography Radiotracers in a Rat Model of Vertebral Discitis-Osteomyelitis
© The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
BACKGROUND: Vertebral discitis-osteomyelitis (VDO) is a devastating infection of the spine that is challenging to distinguish from noninfectious mimics using computed tomography and magnetic resonance imaging. We and others have developed novel metabolism-targeted positron emission tomography (PET) radiotracers for detecting living Staphylococcus aureus and other bacteria in vivo, but their head-to-head performance in a well-validated VDO animal model has not been reported.
METHODS: We compared the performance of several PET radiotracers in a rat model of VDO. [11C]PABA and [18F]FDS were assessed for their ability to distinguish S aureus, the most common non-tuberculous pathogen VDO, from Escherichia coli.
RESULTS: In the rat S aureus VDO model, [11C]PABA could detect as few as 103 bacteria and exhibited the highest signal-to-background ratio, with a 20-fold increased signal in VDO compared to uninfected tissues. In a proof-of-concept experiment, detection of bacterial infection and discrimination between S aureus and E coli was possible using a combination of [11C]PABA and [18F]FDS.
CONCLUSIONS: Our work reveals that several bacteria-targeted PET radiotracers had sufficient signal to background in a rat model of S aureus VDO to be potentially clinically useful. [11C]PABA was the most promising tracer investigated and warrants further investigation in human VDO.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:228 |
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| Enthalten in: |
The Journal of infectious diseases - 228(2023), Suppl 4 vom: 03. Okt., Seite S281-S290 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Parker, Matthew F L [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 05.10.2023 Date Revised 25.04.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/infdis/jiad159 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Parker, Matthew F L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Evaluating the Performance of Pathogen-Targeted Positron Emission Tomography Radiotracers in a Rat Model of Vertebral Discitis-Osteomyelitis |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Completed 05.10.2023 | ||
| 500 | |a Date Revised 25.04.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a BACKGROUND: Vertebral discitis-osteomyelitis (VDO) is a devastating infection of the spine that is challenging to distinguish from noninfectious mimics using computed tomography and magnetic resonance imaging. We and others have developed novel metabolism-targeted positron emission tomography (PET) radiotracers for detecting living Staphylococcus aureus and other bacteria in vivo, but their head-to-head performance in a well-validated VDO animal model has not been reported | ||
| 520 | |a METHODS: We compared the performance of several PET radiotracers in a rat model of VDO. [11C]PABA and [18F]FDS were assessed for their ability to distinguish S aureus, the most common non-tuberculous pathogen VDO, from Escherichia coli | ||
| 520 | |a RESULTS: In the rat S aureus VDO model, [11C]PABA could detect as few as 103 bacteria and exhibited the highest signal-to-background ratio, with a 20-fold increased signal in VDO compared to uninfected tissues. In a proof-of-concept experiment, detection of bacterial infection and discrimination between S aureus and E coli was possible using a combination of [11C]PABA and [18F]FDS | ||
| 520 | |a CONCLUSIONS: Our work reveals that several bacteria-targeted PET radiotracers had sufficient signal to background in a rat model of S aureus VDO to be potentially clinically useful. [11C]PABA was the most promising tracer investigated and warrants further investigation in human VDO | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a S aureus | |
| 650 | 4 | |a Infection imaging | |
| 650 | 4 | |a metabolism | |
| 650 | 4 | |a nuclear medicine | |
| 650 | 4 | |a positron emission tomography | |
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| 650 | 7 | |a 4-Aminobenzoic Acid |2 NLM | |
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| 650 | 7 | |a Radiopharmaceuticals |2 NLM | |
| 700 | 1 | |a López-Álvarez, Marina |e verfasserin |4 aut | |
| 700 | 1 | |a Alanizi, Aryn A |e verfasserin |4 aut | |
| 700 | 1 | |a Luu, Justin M |e verfasserin |4 aut | |
| 700 | 1 | |a Polvoy, Ilona |e verfasserin |4 aut | |
| 700 | 1 | |a Sorlin, Alexandre M |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Hecong |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Sanghee |e verfasserin |4 aut | |
| 700 | 1 | |a Rabbitt, Sarah J |e verfasserin |4 aut | |
| 700 | 1 | |a Pichardo-González, Priamo A |e verfasserin |4 aut | |
| 700 | 1 | |a Ordonez, Alvaro A |e verfasserin |4 aut | |
| 700 | 1 | |a Blecha, Joseph |e verfasserin |4 aut | |
| 700 | 1 | |a Rosenberg, Oren S |e verfasserin |4 aut | |
| 700 | 1 | |a Flavell, Robert R |e verfasserin |4 aut | |
| 700 | 1 | |a Engel, Joanne |e verfasserin |4 aut | |
| 700 | 1 | |a Jain, Sanjay K |e verfasserin |4 aut | |
| 700 | 1 | |a Ohliger, Michael A |e verfasserin |4 aut | |
| 700 | 1 | |a Wilson, David M |e verfasserin |4 aut | |
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