Diagnostic Value and Clinical Application of Metagenomic Next-Generation Sequencing for Infections in Critically Ill Patients
© 2023 He et al..
Objective: To evaluate the diagnostic value and clinical application of metagenomic next-generation sequencing (mNGS) for infections in critically ill patients.
Methods: Comparison of diagnostic performance of mNGS and conventional microbiological testing for pathogens was analyzed in 234 patients. The differences between immunocompetent and immunocompromised individuals in mNGS-guided anti-infective treatment adjustment were also analyzed.
Results: The sensitivity and specificity of mNGS for bacterial and fungal detection were 96.6% (95% confidence interval [CI], 93.5%-99.6%) and 83.1% (95% CI, 75.2%-91.1%), and 85.7% (95% CI, 71.9%-99.5%) and 93.2% (95% CI, 89.7%-96.7%), respectively. Overall, 152 viruses were detected by mNGS, but in which 28 viruses were considered causative agents. The proportion of mNGS-guided beneficial anti-infective therapy adjustments in the immunocompromised group was greater than in the immunocompetent group (48.5% vs 30.1%; P=0.008). In addition, mNGS-guided anti-infective regimens with peripheral blood and BALF specimens had the highest proportion (39.0%; 40.0%), but the proportion of patients not helpful due to peripheral blood mNGS was also as high as 22.0%.
Conclusion: mNGS might be a promising technology to provide precision medicine for critically ill patients with infection.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
Infection and drug resistance - 16(2023) vom: 19., Seite 6309-6322 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
He, Yuxi [VerfasserIn] |
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| Links: |
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| Themen: |
Community-acquired infection |
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| Anmerkungen: |
Date Revised 04.10.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.2147/IDR.S424802 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM362763852 |
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| 520 | |a © 2023 He et al. | ||
| 520 | |a Objective: To evaluate the diagnostic value and clinical application of metagenomic next-generation sequencing (mNGS) for infections in critically ill patients | ||
| 520 | |a Methods: Comparison of diagnostic performance of mNGS and conventional microbiological testing for pathogens was analyzed in 234 patients. The differences between immunocompetent and immunocompromised individuals in mNGS-guided anti-infective treatment adjustment were also analyzed | ||
| 520 | |a Results: The sensitivity and specificity of mNGS for bacterial and fungal detection were 96.6% (95% confidence interval [CI], 93.5%-99.6%) and 83.1% (95% CI, 75.2%-91.1%), and 85.7% (95% CI, 71.9%-99.5%) and 93.2% (95% CI, 89.7%-96.7%), respectively. Overall, 152 viruses were detected by mNGS, but in which 28 viruses were considered causative agents. The proportion of mNGS-guided beneficial anti-infective therapy adjustments in the immunocompromised group was greater than in the immunocompetent group (48.5% vs 30.1%; P=0.008). In addition, mNGS-guided anti-infective regimens with peripheral blood and BALF specimens had the highest proportion (39.0%; 40.0%), but the proportion of patients not helpful due to peripheral blood mNGS was also as high as 22.0% | ||
| 520 | |a Conclusion: mNGS might be a promising technology to provide precision medicine for critically ill patients with infection | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Yang, Tianjun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Chunyan |e verfasserin |4 aut | |
| 700 | 1 | |a Fan, Xiaoqin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yinzhong |e verfasserin |4 aut | |
| 700 | 1 | |a Tong, Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Xiaowei |e verfasserin |4 aut | |
| 700 | 1 | |a Bao, Renren |e verfasserin |4 aut | |
| 700 | 1 | |a Sheng, Ximei |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Aijun |e verfasserin |4 aut | |
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