A lesson from polybutylene succinate plastisphere to the discovery of novel plastic degrading enzyme genes in marine vibrios
© 2023 Applied Microbiology International and John Wiley & Sons Ltd..
Polybutylene succinate (PBS) is an eco-friendly green plastic. However, PBS was shown as being non-biodegradable in marine environments, and up until now, only a limited number of PBS-degrading marine microbes have been discovered. We first set up in vitro PBS- and PBSA (polybutylene succinate adipate)-plastispheres to characterize novel PBS-degrading marine microbes. Microbial growth and oxygen consumption were observed in both PBS- and PBSA-plastispheres enriched with natural seawater collected from Usujiri, Hokkaido, Japan, and Vibrionaceae and Pseudoalteromonadaceae were significantly enriched on these films. Further gene identification indicated that vibrios belonging to the Gazogenes clade possess genes related to a PBS degrading enzyme (PBSase). The PBS degradation assay for six Gazogenes clade vibrios identified Vibrio ruber, Vibrio rhizosphaerae, and Vibrio spartinae as being capable of degrading PBS. We further identified the gene responsible for PBSase from the type strain of V. ruber, and the purified recombinant vibrio PBSase was found to have low-temperature adaptation and was active under high NaCl concentrations. We also provided docking models between the vibrio PBSase and PBS and PBSA units to show how vibrio PBSase interacts with each substrate compared to the Acidovorax PBSase. These results could contribute to a more sustainable society through further utilization of PBS in marine environments and plastic recycling.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
|---|---|
| Enthalten in: |
Environmental microbiology - 25(2023), 12 vom: 19. Dez., Seite 2834-2850 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Kimura, Yutaro [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Completed 20.12.2023 Date Revised 20.12.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/1462-2920.16512 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM362715491 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM362715491 | ||
| 003 | DE-627 | ||
| 005 | 20231227134801.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/1462-2920.16512 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1233.xml |
| 035 | |a (DE-627)NLM362715491 | ||
| 035 | |a (NLM)37775475 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kimura, Yutaro |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A lesson from polybutylene succinate plastisphere to the discovery of novel plastic degrading enzyme genes in marine vibrios |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 20.12.2023 | ||
| 500 | |a Date Revised 20.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 Applied Microbiology International and John Wiley & Sons Ltd. | ||
| 520 | |a Polybutylene succinate (PBS) is an eco-friendly green plastic. However, PBS was shown as being non-biodegradable in marine environments, and up until now, only a limited number of PBS-degrading marine microbes have been discovered. We first set up in vitro PBS- and PBSA (polybutylene succinate adipate)-plastispheres to characterize novel PBS-degrading marine microbes. Microbial growth and oxygen consumption were observed in both PBS- and PBSA-plastispheres enriched with natural seawater collected from Usujiri, Hokkaido, Japan, and Vibrionaceae and Pseudoalteromonadaceae were significantly enriched on these films. Further gene identification indicated that vibrios belonging to the Gazogenes clade possess genes related to a PBS degrading enzyme (PBSase). The PBS degradation assay for six Gazogenes clade vibrios identified Vibrio ruber, Vibrio rhizosphaerae, and Vibrio spartinae as being capable of degrading PBS. We further identified the gene responsible for PBSase from the type strain of V. ruber, and the purified recombinant vibrio PBSase was found to have low-temperature adaptation and was active under high NaCl concentrations. We also provided docking models between the vibrio PBSase and PBS and PBSA units to show how vibrio PBSase interacts with each substrate compared to the Acidovorax PBSase. These results could contribute to a more sustainable society through further utilization of PBS in marine environments and plastic recycling | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a bionole |2 NLM | |
| 650 | 7 | |a Polymers |2 NLM | |
| 650 | 7 | |a Butylene Glycols |2 NLM | |
| 700 | 1 | |a Fukuda, Yutaka |e verfasserin |4 aut | |
| 700 | 1 | |a Otsu, Rumi |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Juwanen |e verfasserin |4 aut | |
| 700 | 1 | |a Mino, Sayaka |e verfasserin |4 aut | |
| 700 | 1 | |a Misawa, Satoru |e verfasserin |4 aut | |
| 700 | 1 | |a Maruyama, Satoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Ikeda, Yuta |e verfasserin |4 aut | |
| 700 | 1 | |a Miyamachi, Remi |e verfasserin |4 aut | |
| 700 | 1 | |a Noguchi, Hiroshi |e verfasserin |4 aut | |
| 700 | 1 | |a Kato, Satoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Yamamoto, Yasuhito |e verfasserin |4 aut | |
| 700 | 1 | |a Sawabe, Tomoo |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Environmental microbiology |d 1999 |g 25(2023), 12 vom: 19. Dez., Seite 2834-2850 |w (DE-627)NLM111123879 |x 1462-2920 |7 nnns |
| 773 | 1 | 8 | |g volume:25 |g year:2023 |g number:12 |g day:19 |g month:12 |g pages:2834-2850 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/1462-2920.16512 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 25 |j 2023 |e 12 |b 19 |c 12 |h 2834-2850 | ||