Discovery and identification of a novel canine coronavirus causing a diarrhea outbreak in Vulpes
Copyright © 2023 Science China Press. Published by Elsevier B.V. All rights reserved..
Cross-species transmission of viruses from wildlife animal reservoirs, such as bats, poses a threat to human and domestic animal health. Previous studies have shown that domestic animals have important roles as intermediate hosts, enabling the transmission of genetically diverse coronaviruses from natural hosts to humans. Here, we report the identification and characterization of a novel canine coronavirus (VuCCoV), which caused an epidemic of acute diarrhea in Vulpes (foxes) in Shenyang, China. The epidemic started on November 8, 2019, and caused more than 39,600 deaths by January 1, 2022. Full-length viral genomic sequences were obtained from 15 foxes with diarrhea at the early stage of this outbreak. The VuCCoV genome shared more than 90% nucleotide identity with canine coronavirus (CCoV) for three of the four structural genes, with the S gene showing a larger amount of divergence. In addition, 67% (10/15) of the VuCCoV genomes contained an open reading frame (ORF3) gene, which was previously only detected in CCoV-I genomes. Notably, VuCCoV had only two to three amino acid differences at the partial RNA-dependent RNA polymerase (RdRp) level to bat CoV, suggesting a close genetic relationship. Therefore, these novel VuCCoV genomes represent a previously unsampled lineage of CCoVs. We also show that the VuCCoV spike protein binds to canine and fox aminopeptidase N (APN), which may allow this protein to serve as an entry receptor. In addition, cell lines were identified that are sensitive to VuCCoV using a pseudovirus system. These data highlight the importance of identifying the diversity and distribution of coronaviruses in domestic animals, which could mitigate future outbreaks that could threaten livestock, public health, and economic growth.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:68 |
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| Enthalten in: |
Science bulletin - 68(2023), 21 vom: 15. Nov., Seite 2598-2606 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Yuting [VerfasserIn] |
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| Links: |
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| Themen: |
Aminopeptidase N |
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| Anmerkungen: |
Date Completed 14.11.2023 Date Revised 24.11.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.scib.2023.09.011 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2023 Science China Press. Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a Cross-species transmission of viruses from wildlife animal reservoirs, such as bats, poses a threat to human and domestic animal health. Previous studies have shown that domestic animals have important roles as intermediate hosts, enabling the transmission of genetically diverse coronaviruses from natural hosts to humans. Here, we report the identification and characterization of a novel canine coronavirus (VuCCoV), which caused an epidemic of acute diarrhea in Vulpes (foxes) in Shenyang, China. The epidemic started on November 8, 2019, and caused more than 39,600 deaths by January 1, 2022. Full-length viral genomic sequences were obtained from 15 foxes with diarrhea at the early stage of this outbreak. The VuCCoV genome shared more than 90% nucleotide identity with canine coronavirus (CCoV) for three of the four structural genes, with the S gene showing a larger amount of divergence. In addition, 67% (10/15) of the VuCCoV genomes contained an open reading frame (ORF3) gene, which was previously only detected in CCoV-I genomes. Notably, VuCCoV had only two to three amino acid differences at the partial RNA-dependent RNA polymerase (RdRp) level to bat CoV, suggesting a close genetic relationship. Therefore, these novel VuCCoV genomes represent a previously unsampled lineage of CCoVs. We also show that the VuCCoV spike protein binds to canine and fox aminopeptidase N (APN), which may allow this protein to serve as an entry receptor. In addition, cell lines were identified that are sensitive to VuCCoV using a pseudovirus system. These data highlight the importance of identifying the diversity and distribution of coronaviruses in domestic animals, which could mitigate future outbreaks that could threaten livestock, public health, and economic growth | ||
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| 700 | 1 | |a Deng, Yao |e verfasserin |4 aut | |
| 700 | 1 | |a Niu, Sheng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Na |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Jingdong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiaoshuang |e verfasserin |4 aut | |
| 700 | 1 | |a Su, Weiting |e verfasserin |4 aut | |
| 700 | 1 | |a Nie, Wenhui |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Roujian |e verfasserin |4 aut | |
| 700 | 1 | |a Irwin, David M |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, George Fu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Wenling |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Qihui |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Wenjie |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Shuyi |e verfasserin |4 aut | |
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