Comparative assessment of the SjSAP4-incorporated gold immunochromatographic assay for the diagnosis of human schistosomiasis japonica
Copyright © 2023 Mu, Rivera, McManus, Weerakoon, Ross, Olveda, Gordon, You, Jones and Cai..
Background: Schistosomiasis, a disease caused by parasites of the genus Schistosoma, remains a global public health threat. This study aimed to validate the diagnostic performance of a recently developed gold immunochromatographic assay (GICA) for the detection of S. japonicum infection in a rural endemic area of the Philippines.
Methods: Human clinical samples were collected from 412 subjects living in Laoang and Palapag municipalities, Northern Samar, the Philippines. The presence of Schistosoma-specific antibodies in serum samples was tested with the SjSAP4-incorporated GICA strips and the results were converted to fully quantitative data by introducing an R value. The performance of the established GICA was further compared with other diagnostic tools, including the Kato-Katz (KK) technique, point-of-care circulating cathodic antigen (POC-CCA), droplet digital (dd) PCR, and enzyme-linked immunosorbent assays (ELISAs).
Results: The developed GICA strip was able to detect KK positive individuals with a sensitivity of 83.3% and absolute specificity. When calibrated with the highly sensitive faecal ddPCR assay, the immunochromatographic assay displayed an accuracy of 60.7%. Globally, the GICA assay showed a high concordance with the SjSAP4-ELISA assay. The schistosomiasis positivity rate determined by the GICA test was similar to those obtained with the SjSAP4-ELISA assay and the ddPCR assay performed on serum samples (SR_ddPCR), and was 2.3 times higher than obtained with the KK method.
Conclusion: The study further confirms that the developed GICA is a valuable diagnostic tool for detecting light S. japonicum infections and implies that this point-of-care assay is a viable solution for surveying endemic areas of low-intensity schistosomiasis and identifying high-priority endemic areas for targeted interventions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Frontiers in public health - 11(2023) vom: 13., Seite 1249637 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Mu, Yi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 25.09.2023 Date Revised 25.09.2023 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.3389/fpubh.2023.1249637 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM362326827 |
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| 100 | 1 | |a Mu, Yi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Comparative assessment of the SjSAP4-incorporated gold immunochromatographic assay for the diagnosis of human schistosomiasis japonica |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Revised 25.09.2023 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Mu, Rivera, McManus, Weerakoon, Ross, Olveda, Gordon, You, Jones and Cai. | ||
| 520 | |a Background: Schistosomiasis, a disease caused by parasites of the genus Schistosoma, remains a global public health threat. This study aimed to validate the diagnostic performance of a recently developed gold immunochromatographic assay (GICA) for the detection of S. japonicum infection in a rural endemic area of the Philippines | ||
| 520 | |a Methods: Human clinical samples were collected from 412 subjects living in Laoang and Palapag municipalities, Northern Samar, the Philippines. The presence of Schistosoma-specific antibodies in serum samples was tested with the SjSAP4-incorporated GICA strips and the results were converted to fully quantitative data by introducing an R value. The performance of the established GICA was further compared with other diagnostic tools, including the Kato-Katz (KK) technique, point-of-care circulating cathodic antigen (POC-CCA), droplet digital (dd) PCR, and enzyme-linked immunosorbent assays (ELISAs) | ||
| 520 | |a Results: The developed GICA strip was able to detect KK positive individuals with a sensitivity of 83.3% and absolute specificity. When calibrated with the highly sensitive faecal ddPCR assay, the immunochromatographic assay displayed an accuracy of 60.7%. Globally, the GICA assay showed a high concordance with the SjSAP4-ELISA assay. The schistosomiasis positivity rate determined by the GICA test was similar to those obtained with the SjSAP4-ELISA assay and the ddPCR assay performed on serum samples (SR_ddPCR), and was 2.3 times higher than obtained with the KK method | ||
| 520 | |a Conclusion: The study further confirms that the developed GICA is a valuable diagnostic tool for detecting light S. japonicum infections and implies that this point-of-care assay is a viable solution for surveying endemic areas of low-intensity schistosomiasis and identifying high-priority endemic areas for targeted interventions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a ASSURED criteria | |
| 650 | 4 | |a POC-CCA | |
| 650 | 4 | |a Schistosoma japonicum | |
| 650 | 4 | |a SjSAP4 | |
| 650 | 4 | |a diagnosis | |
| 650 | 4 | |a droplet digital PCR | |
| 650 | 4 | |a gold immunochromatographic assay (GICA) | |
| 650 | 4 | |a schistosomiasis | |
| 650 | 7 | |a Gold |2 NLM | |
| 650 | 7 | |a 7440-57-5 |2 NLM | |
| 700 | 1 | |a Rivera, Jonas |e verfasserin |4 aut | |
| 700 | 1 | |a McManus, Donald P |e verfasserin |4 aut | |
| 700 | 1 | |a Weerakoon, Kosala G |e verfasserin |4 aut | |
| 700 | 1 | |a Ross, Allen G |e verfasserin |4 aut | |
| 700 | 1 | |a Olveda, Remigio M |e verfasserin |4 aut | |
| 700 | 1 | |a Gordon, Catherine A |e verfasserin |4 aut | |
| 700 | 1 | |a You, Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Jones, Malcolm K |e verfasserin |4 aut | |
| 700 | 1 | |a Cai, Pengfei |e verfasserin |4 aut | |
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