A novel array of real-time RT-PCR assays for the rapid pathotyping of type I avian paramyxovirus (APMV-1)
Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved..
Newcastle disease (ND) caused by virulent avian paramyxovirus type I (APMV-1) is a WOAH and EU listed disease affecting poultry worldwide. ND exhibits different clinical manifestations that may either be neurological, respiratory and/or gastrointestinal, accompanied by high mortality. In contrast, mild or subclinical forms are generally caused by lentogenic APMV-1 and are not subject to notification. The rapid discrimination of virulent and avirulent viruses is paramount to limit the spread of virulent APMV-1. The appropriateness of molecular methods for APMV-1 pathotyping is often hampered by the high genetic variability of these viruses that affects sensitivity and inclusivity. This work presents a new array of real-time RT-PCR (RT-qPCR) assays that enable the identification of virulent and avirulent viruses in dual mode, i.e., through pathotype-specific probes and subsequent Sanger sequencing of the amplification product. Validation was performed according to the WOAH recommendations. Performance indicators on sensitivity, specificity, repeatability and reproducibility yielded favourable results. Reproducibility highlighted the need for assays optimization whenever major changes are made to the procedure. Overall, the new RT-qPCRs showed its ability to detect and pathotype all tested APMV-1 genotypes and its suitability for routine use in clinical samples.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:322 |
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| Enthalten in: |
Journal of virological methods - 322(2023) vom: 01. Dez., Seite 114813 |
| Sprache: |
Englisch |
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| Links: |
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| Themen: |
Avian paramyxovirus type I (APMV-1) |
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| Anmerkungen: |
Date Completed 13.11.2023 Date Revised 18.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jviromet.2023.114813 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 2 | |a A novel array of real-time RT-PCR assays for the rapid pathotyping of type I avian paramyxovirus (APMV-1) |
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| 520 | |a Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a Newcastle disease (ND) caused by virulent avian paramyxovirus type I (APMV-1) is a WOAH and EU listed disease affecting poultry worldwide. ND exhibits different clinical manifestations that may either be neurological, respiratory and/or gastrointestinal, accompanied by high mortality. In contrast, mild or subclinical forms are generally caused by lentogenic APMV-1 and are not subject to notification. The rapid discrimination of virulent and avirulent viruses is paramount to limit the spread of virulent APMV-1. The appropriateness of molecular methods for APMV-1 pathotyping is often hampered by the high genetic variability of these viruses that affects sensitivity and inclusivity. This work presents a new array of real-time RT-PCR (RT-qPCR) assays that enable the identification of virulent and avirulent viruses in dual mode, i.e., through pathotype-specific probes and subsequent Sanger sequencing of the amplification product. Validation was performed according to the WOAH recommendations. Performance indicators on sensitivity, specificity, repeatability and reproducibility yielded favourable results. Reproducibility highlighted the need for assays optimization whenever major changes are made to the procedure. Overall, the new RT-qPCRs showed its ability to detect and pathotype all tested APMV-1 genotypes and its suitability for routine use in clinical samples | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Avian paramyxovirus type I (APMV-1) | |
| 650 | 4 | |a Avirulent | |
| 650 | 4 | |a Molecular pathotyping | |
| 650 | 4 | |a Newcastle disease | |
| 650 | 4 | |a Virulent | |
| 700 | 1 | |a Laconi, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Monne, Isabella |e verfasserin |4 aut | |
| 700 | 1 | |a Zohari, Siamak |e verfasserin |4 aut | |
| 700 | 1 | |a Andersson, Kristofer |e verfasserin |4 aut | |
| 700 | 1 | |a Grund, Christian |e verfasserin |4 aut | |
| 700 | 1 | |a Cecchinato, Mattia |e verfasserin |4 aut | |
| 700 | 1 | |a Crimaudo, Marika |e verfasserin |4 aut | |
| 700 | 1 | |a Valastro, Viviana |e verfasserin |4 aut | |
| 700 | 1 | |a D'Amico, Valeria |e verfasserin |4 aut | |
| 700 | 1 | |a Bortolami, Alessio |e verfasserin |4 aut | |
| 700 | 1 | |a Gastaldelli, Michele |e verfasserin |4 aut | |
| 700 | 1 | |a Varotto, Maria |e verfasserin |4 aut | |
| 700 | 0 | |a Newcastle Disease Collaborating Diagnostic Group |e verfasserin |4 aut | |
| 700 | 1 | |a Terregino, Calogero |e verfasserin |4 aut | |
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| 700 | 1 | |a Belayneh, Redeat |e investigator |4 oth | |
| 700 | 1 | |a Cyprien, Yapi Bokpè |e investigator |4 oth | |
| 700 | 1 | |a Christodoulou, Vasiliki |e investigator |4 oth | |
| 700 | 1 | |a Chvala, Ilya |e investigator |4 oth | |
| 700 | 1 | |a Dodovski, Aleksandar |e investigator |4 oth | |
| 700 | 1 | |a Ghafouri, Seyed Ali |e investigator |4 oth | |
| 700 | 1 | |a Giasuddin, Mohammed |e investigator |4 oth | |
| 700 | 1 | |a Hassan, Magdy |e investigator |4 oth | |
| 700 | 1 | |a Kammon, Abdulwahab |e investigator |4 oth | |
| 700 | 1 | |a Shittu, Ismaila |e investigator |4 oth | |
| 700 | 1 | |a Snoeck, Chantal J |e investigator |4 oth | |
| 700 | 1 | |a Steensel, Mieke |e investigator |4 oth | |
| 700 | 1 | |a Suarez, David |e investigator |4 oth | |
| 700 | 1 | |a Torchetti, Mia Kim |e investigator |4 oth | |
| 700 | 1 | |a Tshipambe, Serge Mpiana |e investigator |4 oth | |
| 700 | 1 | |a Ouermi Zerbo, Lamouni Habibata |e investigator |4 oth | |
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