Laboratory evaluation of ELISA and indirect immunofluorescence assay in response to emergence of Japanese encephalitis virus genotype IV in Australia
Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved..
The unexpected recent emergence of Japanese encephalitis virus (JEV) genotype IV in multiple southern states of Australia necessitated an evaluation of JEV serological tests suitable for diagnosing acute infection and for seroprevalence studies. This study examined the analytical and clinical performance of two high-throughput JEV assays, Euroimmun immunofluorescence assay (IFA) and Euroimmun enzyme-linked immunosorbent assay (ELISA), across four cohorts; (1) surveillance of piggery workers in outbreak areas, (2) surveillance of residents in outbreak areas, (3) acute JEV infection and (4) post-JEV vaccination. ELISA and IFA IgM demonstrated minimal cross-reactivity (0-1.8%) with other endemic flaviviruses, with high sensitivity (100%) for acute JEV infection in this low endemicity setting. Differences in IgG serodynamics between the two assays suggest convalescent and paired testing with IgM are critical in diagnosing acute infection. High assay concordance was observed between ELISA and IFA when used in serosurveillance (97.4% agreement, Cohen' κ 0.74 [95% CI 0.614-0.860]) and vaccination cohorts (91.1% agreement, Cohen's κ 0.806 [95% CI 0.672-0.941]). In conclusion, this study highlights the clinical & epidemiological applications and limitations of these two commercial JEV assays.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:168 |
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| Enthalten in: |
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 168(2023) vom: 05. Nov., Seite 105580 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kinsella, Paul [VerfasserIn] |
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| Links: |
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| Themen: |
ELISA |
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| Anmerkungen: |
Date Revised 12.10.2023 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1016/j.jcv.2023.105580 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM36214513X |
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| 520 | |a Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a The unexpected recent emergence of Japanese encephalitis virus (JEV) genotype IV in multiple southern states of Australia necessitated an evaluation of JEV serological tests suitable for diagnosing acute infection and for seroprevalence studies. This study examined the analytical and clinical performance of two high-throughput JEV assays, Euroimmun immunofluorescence assay (IFA) and Euroimmun enzyme-linked immunosorbent assay (ELISA), across four cohorts; (1) surveillance of piggery workers in outbreak areas, (2) surveillance of residents in outbreak areas, (3) acute JEV infection and (4) post-JEV vaccination. ELISA and IFA IgM demonstrated minimal cross-reactivity (0-1.8%) with other endemic flaviviruses, with high sensitivity (100%) for acute JEV infection in this low endemicity setting. Differences in IgG serodynamics between the two assays suggest convalescent and paired testing with IgM are critical in diagnosing acute infection. High assay concordance was observed between ELISA and IFA when used in serosurveillance (97.4% agreement, Cohen' κ 0.74 [95% CI 0.614-0.860]) and vaccination cohorts (91.1% agreement, Cohen's κ 0.806 [95% CI 0.672-0.941]). In conclusion, this study highlights the clinical & epidemiological applications and limitations of these two commercial JEV assays | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a ELISA | |
| 650 | 4 | |a Flavivirus | |
| 650 | 4 | |a Immunofluorescence | |
| 650 | 4 | |a Japanese encephalitis virus | |
| 650 | 4 | |a Serology | |
| 700 | 1 | |a Moso, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Martin, Genevieve |e verfasserin |4 aut | |
| 700 | 1 | |a Karapangiotidis, Theo |e verfasserin |4 aut | |
| 700 | 1 | |a Karamalakis, Di |e verfasserin |4 aut | |
| 700 | 1 | |a Nicholson, Suellen |e verfasserin |4 aut | |
| 700 | 1 | |a Batty, Mitch |e verfasserin |4 aut | |
| 700 | 1 | |a Jackson, Kathy |e verfasserin |4 aut | |
| 700 | 1 | |a Marsland, Madeleine |e verfasserin |4 aut | |
| 700 | 1 | |a Thomson, Tilda |e verfasserin |4 aut | |
| 700 | 1 | |a Manoharan, Lakshmi |e verfasserin |4 aut | |
| 700 | 1 | |a O'brien, Helen |e verfasserin |4 aut | |
| 700 | 1 | |a Friedman, N Deborah |e verfasserin |4 aut | |
| 700 | 1 | |a Bond, Katherine |e verfasserin |4 aut | |
| 700 | 1 | |a Williamson, Deborah A |e verfasserin |4 aut | |
| 700 | 1 | |a Lim, Chuan Kok |e verfasserin |4 aut | |
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