Comprehensive analysis of OsJAZ gene family deciphers rhizobacteria-mediated nutrient stress modulation in rice
Copyright © 2023 Elsevier B.V. All rights reserved..
The JASMONATE-ZIM DOMAIN (JAZ) repressors are crucial proteins in jasmonic acid signaling pathway that are critical for plant growth. Therefore, the present study aimed to identify and characterize OsJAZs in the rice genome, revealing their structural attributes, regulatory elements, miRNA interactions, and subcellular localization. 23 JAZ transcripts across the 6 chromosomes of rice genome were identified having conserved domains and different physiochemical characteristics. Phylogenetically classified into five clades, they showed highest syntenic relationship with P. virgatum. The non-synonymous/synonymous values ranged from 0.44 to 1.21 suggesting purifying/stabilizing selection in OsJAZs. The study examined the 1.5 kb promoter region for cis-regulatory elements, and also identified 92 miRNAs targets. Furthermore, homology modeling provided insights into the 3D-structures of JAZ proteins while in-silico gene expression analysis revealed their functional diversity in various tissues and developmental stages. Additionally, qRT-PCR analysis highlighted their involvement in stress adaptation to sub-optimum nutrient conditions induced by plant-beneficial rhizobacteria Bacillus amyloliquefaciens (SN13) in two rice varieties. Distinct OsJAZ expression patterns in the two varieties correlated with altered root architecture, xylem structure, and lignification. These findings affirmed that specific up-or down-regulation of OsJAZs might play critical role in SN13 induced changes in the two varieties that enabled them to survive under stress.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:253 |
|---|---|
| Enthalten in: |
International journal of biological macromolecules - 253(2023), Pt 3 vom: 31. Dez., Seite 126832 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Bisht, Nikita [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Genome-wide analysis |
|---|
| Anmerkungen: |
Date Completed 24.11.2023 Date Revised 24.11.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.ijbiomac.2023.126832 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM362064679 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM362064679 | ||
| 003 | DE-627 | ||
| 005 | 20231226090445.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ijbiomac.2023.126832 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1206.xml |
| 035 | |a (DE-627)NLM362064679 | ||
| 035 | |a (NLM)37709234 | ||
| 035 | |a (PII)S0141-8130(23)03729-7 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Bisht, Nikita |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Comprehensive analysis of OsJAZ gene family deciphers rhizobacteria-mediated nutrient stress modulation in rice |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 24.11.2023 | ||
| 500 | |a Date Revised 24.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a The JASMONATE-ZIM DOMAIN (JAZ) repressors are crucial proteins in jasmonic acid signaling pathway that are critical for plant growth. Therefore, the present study aimed to identify and characterize OsJAZs in the rice genome, revealing their structural attributes, regulatory elements, miRNA interactions, and subcellular localization. 23 JAZ transcripts across the 6 chromosomes of rice genome were identified having conserved domains and different physiochemical characteristics. Phylogenetically classified into five clades, they showed highest syntenic relationship with P. virgatum. The non-synonymous/synonymous values ranged from 0.44 to 1.21 suggesting purifying/stabilizing selection in OsJAZs. The study examined the 1.5 kb promoter region for cis-regulatory elements, and also identified 92 miRNAs targets. Furthermore, homology modeling provided insights into the 3D-structures of JAZ proteins while in-silico gene expression analysis revealed their functional diversity in various tissues and developmental stages. Additionally, qRT-PCR analysis highlighted their involvement in stress adaptation to sub-optimum nutrient conditions induced by plant-beneficial rhizobacteria Bacillus amyloliquefaciens (SN13) in two rice varieties. Distinct OsJAZ expression patterns in the two varieties correlated with altered root architecture, xylem structure, and lignification. These findings affirmed that specific up-or down-regulation of OsJAZs might play critical role in SN13 induced changes in the two varieties that enabled them to survive under stress | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Genome-wide analysis | |
| 650 | 4 | |a JAZ gene family | |
| 650 | 4 | |a Nutrient deficiency | |
| 650 | 4 | |a PGPR | |
| 650 | 4 | |a Root system architecture | |
| 650 | 7 | |a Transcription Factors |2 NLM | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 700 | 1 | |a Anshu, Anshu |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Poonam C |e verfasserin |4 aut | |
| 700 | 1 | |a Chauhan, Puneet Singh |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of biological macromolecules |d 1992 |g 253(2023), Pt 3 vom: 31. Dez., Seite 126832 |w (DE-627)NLM012627356 |x 1879-0003 |7 nnns |
| 773 | 1 | 8 | |g volume:253 |g year:2023 |g number:Pt 3 |g day:31 |g month:12 |g pages:126832 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.ijbiomac.2023.126832 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 253 |j 2023 |e Pt 3 |b 31 |c 12 |h 126832 | ||