Male vitellogenin regulates gametogenesis through a testis-enriched big protein in Chrysopa pallens
© 2023 Royal Entomological Society..
In insects, vitellogenin (Vg) is generally viewed as a female-specific protein. Its primary function is to supply nutrition to developing embryos. Here, we reported Vg from the male adults of a natural predator, Chrysopa pallens. The male Vg was depleted by RNAi. Mating with Vg-deficient male downregulated female Vg expression, suppressed ovarian development and decreased reproductive output. Whole-organism transcriptome analysis after male Vg knockdown showed no differential expression of the known spermatogenesis-related regulators and seminal fluid protein genes, but a sharp downregulation of an unknown gene, which encodes a testis-enriched big protein (Vcsoo). Separate knockdown of male Vg and Vcsoo disturbed the assembly of spermatid cytoplasmic organelles in males and suppressed the expansion of ovary germarium in mated females. These results demonstrated that C. pallens male Vg signals through the downstream Vcsoo and regulates male and female reproduction.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
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| Enthalten in: |
Insect molecular biology - 33(2024), 1 vom: 08. Jan., Seite 17-28 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Xiao-Ping [VerfasserIn] |
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| Links: |
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| Themen: |
C. pallens |
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| Anmerkungen: |
Date Completed 10.01.2024 Date Revised 10.01.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/imb.12873 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM362045453 |
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| 520 | |a © 2023 Royal Entomological Society. | ||
| 520 | |a In insects, vitellogenin (Vg) is generally viewed as a female-specific protein. Its primary function is to supply nutrition to developing embryos. Here, we reported Vg from the male adults of a natural predator, Chrysopa pallens. The male Vg was depleted by RNAi. Mating with Vg-deficient male downregulated female Vg expression, suppressed ovarian development and decreased reproductive output. Whole-organism transcriptome analysis after male Vg knockdown showed no differential expression of the known spermatogenesis-related regulators and seminal fluid protein genes, but a sharp downregulation of an unknown gene, which encodes a testis-enriched big protein (Vcsoo). Separate knockdown of male Vg and Vcsoo disturbed the assembly of spermatid cytoplasmic organelles in males and suppressed the expansion of ovary germarium in mated females. These results demonstrated that C. pallens male Vg signals through the downstream Vcsoo and regulates male and female reproduction | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a oogenesis | |
| 650 | 4 | |a spermatogenesis | |
| 650 | 4 | |a vitellogenin | |
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| 700 | 1 | |a Feng, Yan-Jiao |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Xing-Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Li-Sheng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Meng-Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yu-Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Zeng, Fan-Rong |e verfasserin |4 aut | |
| 700 | 1 | |a Nolan, Tony |e verfasserin |4 aut | |
| 700 | 1 | |a Mao, Jian-Jun |e verfasserin |4 aut | |
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