Identification of virulence-associated factors in Vibrio parahaemolyticus with special reference to moonlighting protein : a secretomics study
© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG..
Vibrio parahaemolyticus causes seafood-borne gastroenteritis infection in human which can even lead to death. The pathogenic strain of V. parahaemolyticus secretes different types of virulence factors that are directly injected into the host cell by a different type of secretion system which helps bacteria to establish its own ecological niche within the organism. Therefore, the aim of this study was to isolate the extracellular secreted proteins from the trh positive strain of V. parahaemolyticus and identify them using two-dimensional gel electrophoresis and MALDI-TOFMS/MS. Seventeen different cellular proteins viz, Carbamoyl-phosphate synthase, 5-methyltetrahydropteroyltriglutamate, tRNA-dihydrouridine synthase, Glycerol-3-phosphate dehydrogenase, Orotidine 5'-phosphate decarboxylase, Molybdenum import ATP-binding protein, DnaJ, DNA polymerase IV, Ribosomal RNA small subunit methyltransferase G, ATP synthase subunit delta and gamma, Ribosome-recycling factor, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, tRNA pseudouridine synthase B, Ditrans, polycis-undecaprenyl-diphosphate synthase, Oxygen-dependent coproporphyrinogen-III oxidase, and Peptide deformylase 2 were identified which are mainly involved in different metabolic and biosynthetic pathways. Furthermore, the molecular function of the identified proteins were associated with catalytic activity, ligase activity, transporter, metal binding, and ATP synthase when they are intercellular. However, to understand the importance of these secreted proteins in the infection and survival of bacteria inside the host cell, pathogen-host protein-protein interactions (PPIs) were carried out which identified the association of eight secreted proteins with 41 human proteins involved in different cellular pathways, including ubiquitination degradation, adhesion, inflammation, immunity, and programmed cell death. The present study provides unreported strategies on host-cell environment's survival and adaptation mechanisms for the successful establishment of infections and intracellular propagation.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2023 |
|---|---|
| Enthalten in: |
International microbiology : the official journal of the Spanish Society for Microbiology - (2023) vom: 13. Sept. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Paria, Prasenjit [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Host–pathogen interactions |
|---|
| Anmerkungen: |
Date Revised 13.09.2023 published: Print-Electronic Citation Status Publisher |
|---|
| doi: |
10.1007/s10123-023-00429-y |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM362001790 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM362001790 | ||
| 003 | DE-627 | ||
| 005 | 20231226090329.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10123-023-00429-y |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1206.xml |
| 035 | |a (DE-627)NLM362001790 | ||
| 035 | |a (NLM)37702858 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Paria, Prasenjit |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification of virulence-associated factors in Vibrio parahaemolyticus with special reference to moonlighting protein |b a secretomics study |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 13.09.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a © 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG. | ||
| 520 | |a Vibrio parahaemolyticus causes seafood-borne gastroenteritis infection in human which can even lead to death. The pathogenic strain of V. parahaemolyticus secretes different types of virulence factors that are directly injected into the host cell by a different type of secretion system which helps bacteria to establish its own ecological niche within the organism. Therefore, the aim of this study was to isolate the extracellular secreted proteins from the trh positive strain of V. parahaemolyticus and identify them using two-dimensional gel electrophoresis and MALDI-TOFMS/MS. Seventeen different cellular proteins viz, Carbamoyl-phosphate synthase, 5-methyltetrahydropteroyltriglutamate, tRNA-dihydrouridine synthase, Glycerol-3-phosphate dehydrogenase, Orotidine 5'-phosphate decarboxylase, Molybdenum import ATP-binding protein, DnaJ, DNA polymerase IV, Ribosomal RNA small subunit methyltransferase G, ATP synthase subunit delta and gamma, Ribosome-recycling factor, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, tRNA pseudouridine synthase B, Ditrans, polycis-undecaprenyl-diphosphate synthase, Oxygen-dependent coproporphyrinogen-III oxidase, and Peptide deformylase 2 were identified which are mainly involved in different metabolic and biosynthetic pathways. Furthermore, the molecular function of the identified proteins were associated with catalytic activity, ligase activity, transporter, metal binding, and ATP synthase when they are intercellular. However, to understand the importance of these secreted proteins in the infection and survival of bacteria inside the host cell, pathogen-host protein-protein interactions (PPIs) were carried out which identified the association of eight secreted proteins with 41 human proteins involved in different cellular pathways, including ubiquitination degradation, adhesion, inflammation, immunity, and programmed cell death. The present study provides unreported strategies on host-cell environment's survival and adaptation mechanisms for the successful establishment of infections and intracellular propagation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Host–pathogen interactions | |
| 650 | 4 | |a Moonlighting proteins | |
| 650 | 4 | |a Pathogenicity | |
| 650 | 4 | |a Proteomics | |
| 650 | 4 | |a Secretomics | |
| 650 | 4 | |a Vibrio parahaemolyticus | |
| 700 | 1 | |a Chakraborty, Hirak Jyoti |e verfasserin |4 aut | |
| 700 | 1 | |a Pakhira, Abhijit |e verfasserin |4 aut | |
| 700 | 1 | |a Devi, Manoharmayum Shaya |e verfasserin |4 aut | |
| 700 | 1 | |a Das Mohapatra, Pradeep Kumar |e verfasserin |4 aut | |
| 700 | 1 | |a Behera, Bijay Kumar |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International microbiology : the official journal of the Spanish Society for Microbiology |d 1998 |g (2023) vom: 13. Sept. |w (DE-627)NLM097544469 |x 1618-1905 |7 nnns |
| 773 | 1 | 8 | |g year:2023 |g day:13 |g month:09 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s10123-023-00429-y |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 13 |c 09 | ||