Quantitative Assessment of Peripheral Oxidative Metabolism With a New Dynamic 1 H MRI Technique : A Pilot Study in People With and Without Diabetes Mellitus
© 2023 International Society for Magnetic Resonance in Medicine..
BACKGROUND: Type 2 diabetes mellitus (T2DM) is linked to impaired mitochondrial function. Chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) is a gadolinium-contrast-free 1 H method to assess mitochondrial function by measuring low-concentration metabolites. A CEST MRI-based technique may serve as a non-invasive proxy for assessing mitochondrial health.
HYPOTHESIS: A 1 H CEST MRI technique may detect significant differences in in vivo skeletal muscle phosphocreatine (SMPCr) kinetics between healthy volunteers and T2DM patients undergoing standardized isometric exercise.
STUDY TYPE: Cross-sectional study.
SUBJECTS: Seven subjects without T2DM (T2DM-) and seven age, sex, and BMI-matched subjects with T2DM (T2DM+).
FIELD STRENGTH/SEQUENCE: Single-shot rapid acquisition with refocusing echoes (RARE) and single-shot gradient-echo sequences, 3 T.
ASSESSMENT: Subjects underwent a rest-exercise-recovery imaging protocol to dynamically acquire SMPCr maps in calf musculature. Medial gastrocnemius (MG) and soleus SMPCr concentrations were plotted over time, and SMPCr recovery time, τ $$ \tau $$ , was determined. Mitochondrial function index was calculated as the ratio of resting SMPCr to τ $$ \tau $$ . Participants underwent a second exercise protocol for imaging of skeletal muscle blood flow (SMBF), and its association with SMPCr was assessed.
STATISTICAL TESTS: Unpaired t-tests and Pearson correlation coefficient. A P value <0.05 was considered statistically significant.
RESULTS: SMPCr concentrations in MG and soleus displayed expected declines during exercise and returns to baseline during recovery. τ $$ \tau $$ was significantly longer in the T2DM+ cohort (MG 83.5 ± 25.8 vs. 54.0 ± 21.1, soleus 90.5 ± 18.9 vs. 51.2 ± 14.5). The mitochondrial function index in the soleus was significantly lower in the T2DM+ cohort (0.33 ± 0.08 vs. 0.66 ± 0.19). SMBF was moderately correlated with the SMPCr in T2DM-; this correlation was not significant in T2DM+ (r = -0.23, P = 0.269).
CONCLUSION: The CEST MRI method is feasible for quantifying SMPCr in peripheral muscle tissue. T2DM+ individuals had significantly lower oxidative capacities than T2DM- individuals. In T2DM, skeletal muscle metabolism appeared to be decoupled from perfusion.
LEVEL OF EVIDENCE: 1 TECHNICAL EFFICACY: Stage 1.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2023 |
|---|---|
| Enthalten in: |
Journal of magnetic resonance imaging : JMRI - (2023) vom: 11. Sept. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wahidi, Ryan [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Blood flow |
|---|
| Anmerkungen: |
Date Revised 12.03.2024 published: Print-Electronic Citation Status Publisher |
|---|
| doi: |
10.1002/jmri.28996 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM361925484 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM361925484 | ||
| 003 | DE-627 | ||
| 005 | 20240312233219.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/jmri.28996 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1324.xml |
| 035 | |a (DE-627)NLM361925484 | ||
| 035 | |a (NLM)37695103 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wahidi, Ryan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Quantitative Assessment of Peripheral Oxidative Metabolism With a New Dynamic 1 H MRI Technique |b A Pilot Study in People With and Without Diabetes Mellitus |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 12.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a © 2023 International Society for Magnetic Resonance in Medicine. | ||
| 520 | |a BACKGROUND: Type 2 diabetes mellitus (T2DM) is linked to impaired mitochondrial function. Chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) is a gadolinium-contrast-free 1 H method to assess mitochondrial function by measuring low-concentration metabolites. A CEST MRI-based technique may serve as a non-invasive proxy for assessing mitochondrial health | ||
| 520 | |a HYPOTHESIS: A 1 H CEST MRI technique may detect significant differences in in vivo skeletal muscle phosphocreatine (SMPCr) kinetics between healthy volunteers and T2DM patients undergoing standardized isometric exercise | ||
| 520 | |a STUDY TYPE: Cross-sectional study | ||
| 520 | |a SUBJECTS: Seven subjects without T2DM (T2DM-) and seven age, sex, and BMI-matched subjects with T2DM (T2DM+) | ||
| 520 | |a FIELD STRENGTH/SEQUENCE: Single-shot rapid acquisition with refocusing echoes (RARE) and single-shot gradient-echo sequences, 3 T | ||
| 520 | |a ASSESSMENT: Subjects underwent a rest-exercise-recovery imaging protocol to dynamically acquire SMPCr maps in calf musculature. Medial gastrocnemius (MG) and soleus SMPCr concentrations were plotted over time, and SMPCr recovery time, τ $$ \tau $$ , was determined. Mitochondrial function index was calculated as the ratio of resting SMPCr to τ $$ \tau $$ . Participants underwent a second exercise protocol for imaging of skeletal muscle blood flow (SMBF), and its association with SMPCr was assessed | ||
| 520 | |a STATISTICAL TESTS: Unpaired t-tests and Pearson correlation coefficient. A P value <0.05 was considered statistically significant | ||
| 520 | |a RESULTS: SMPCr concentrations in MG and soleus displayed expected declines during exercise and returns to baseline during recovery. τ $$ \tau $$ was significantly longer in the T2DM+ cohort (MG 83.5 ± 25.8 vs. 54.0 ± 21.1, soleus 90.5 ± 18.9 vs. 51.2 ± 14.5). The mitochondrial function index in the soleus was significantly lower in the T2DM+ cohort (0.33 ± 0.08 vs. 0.66 ± 0.19). SMBF was moderately correlated with the SMPCr in T2DM-; this correlation was not significant in T2DM+ (r = -0.23, P = 0.269) | ||
| 520 | |a CONCLUSION: The CEST MRI method is feasible for quantifying SMPCr in peripheral muscle tissue. T2DM+ individuals had significantly lower oxidative capacities than T2DM- individuals. In T2DM, skeletal muscle metabolism appeared to be decoupled from perfusion | ||
| 520 | |a LEVEL OF EVIDENCE: 1 TECHNICAL EFFICACY: Stage 1 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a blood flow | |
| 650 | 4 | |a diabetes mellitus | |
| 650 | 4 | |a phosphocreatine | |
| 650 | 4 | |a skeletal muscle | |
| 700 | 1 | |a Zhang, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Ran |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Jiadi |e verfasserin |4 aut | |
| 700 | 1 | |a Zayed, Mohamed A |e verfasserin |4 aut | |
| 700 | 1 | |a Hastings, Mary K |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Jie |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of magnetic resonance imaging : JMRI |d 1996 |g (2023) vom: 11. Sept. |w (DE-627)NLM013085476 |x 1522-2586 |7 nnns |
| 773 | 1 | 8 | |g year:2023 |g day:11 |g month:09 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/jmri.28996 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 11 |c 09 | ||