The C2H2 zinc finger transcription factor CF2-II regulates multi-insecticide resistance-related gut-predominant ABC transporters in Aphis gossypii Glover
Copyright © 2023 Elsevier B.V. All rights reserved..
Clarifying the molecular mechanisms of cotton aphid resistance to various insecticides is crucial for the long-term safe application of insecticides in chemical control. ATP-binding cassette (ABC) transporters mediate the membrane transport of various substrates (including exogenous substances). Experiments confirmed that ABCB5, ABCF2, and MRP12 contributed to high levels of resistance to spirotetramat, cyantraniliprole, thiamethoxam or imidacloprid. Binding sites of the C2H2 zinc finger transcription factor CF2-II was predicted to be located in the promoters of ABCB5, ABCF2, and MRP12. The expression levels of ABCB5, ABCF2, and MRP12 were significantly upregulated after silencing CF2-II. The results of dual-luciferase reporter assays demonstrated a negative regulatory relationship between CF2-II and ABC transporter promoters. Furthermore, yeast one-hybrid (Y1H) and electrophoresis mobility shift assays (EMSAs) revealed that CF2-II inhibited the expression of ABC transporter genes through interaction with binding sites [ABCF2.p (-1149/-1140) or MRP12.p (-1189/-1181)]. The above results indicated that ABCB5, ABCF2, and MRP12 were negatively regulated by the transcription factor CF2-II, which will help us further understand the mechanism of transcriptional adaption of multi-insecticides resistant related ABC transporters in response to xenobiotics.
| Errataetall: |
ErratumIn: Int J Biol Macromol. 2023 Nov 28;256(Pt 1):128361. - PMID 38029529 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:253 |
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| Enthalten in: |
International journal of biological macromolecules - 253(2023), Pt 2 vom: 31. Dez., Seite 126765 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lv, Yuntong [VerfasserIn] |
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| Links: |
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| Themen: |
9001-26-7 |
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| Anmerkungen: |
Date Completed 23.11.2023 Date Revised 29.11.2023 published: Print-Electronic ErratumIn: Int J Biol Macromol. 2023 Nov 28;256(Pt 1):128361. - PMID 38029529 Citation Status MEDLINE |
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| doi: |
10.1016/j.ijbiomac.2023.126765 |
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| funding: |
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| 245 | 1 | 4 | |a The C2H2 zinc finger transcription factor CF2-II regulates multi-insecticide resistance-related gut-predominant ABC transporters in Aphis gossypii Glover |
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| 500 | |a ErratumIn: Int J Biol Macromol. 2023 Nov 28;256(Pt 1):128361. - PMID 38029529 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a Clarifying the molecular mechanisms of cotton aphid resistance to various insecticides is crucial for the long-term safe application of insecticides in chemical control. ATP-binding cassette (ABC) transporters mediate the membrane transport of various substrates (including exogenous substances). Experiments confirmed that ABCB5, ABCF2, and MRP12 contributed to high levels of resistance to spirotetramat, cyantraniliprole, thiamethoxam or imidacloprid. Binding sites of the C2H2 zinc finger transcription factor CF2-II was predicted to be located in the promoters of ABCB5, ABCF2, and MRP12. The expression levels of ABCB5, ABCF2, and MRP12 were significantly upregulated after silencing CF2-II. The results of dual-luciferase reporter assays demonstrated a negative regulatory relationship between CF2-II and ABC transporter promoters. Furthermore, yeast one-hybrid (Y1H) and electrophoresis mobility shift assays (EMSAs) revealed that CF2-II inhibited the expression of ABC transporter genes through interaction with binding sites [ABCF2.p (-1149/-1140) or MRP12.p (-1189/-1181)]. The above results indicated that ABCB5, ABCF2, and MRP12 were negatively regulated by the transcription factor CF2-II, which will help us further understand the mechanism of transcriptional adaption of multi-insecticides resistant related ABC transporters in response to xenobiotics | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a ABC transporters | |
| 650 | 4 | |a Aphis gossypii | |
| 650 | 4 | |a C2H2 zinc finger | |
| 650 | 4 | |a Resistance | |
| 650 | 4 | |a Transcription factor | |
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| 650 | 7 | |a ATP-Binding Cassette Transporters |2 NLM | |
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| 700 | 1 | |a Li, Jianyi |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Yaping |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Zihan |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Kunpeng |e verfasserin |4 aut | |
| 700 | 1 | |a Shang, Qingli |e verfasserin |4 aut | |
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