Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines
© 2023 The Author(s)..
IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both are influenced by the composition of the conserved N-linked glycan located in the IgG Fc domain. Here, we compared the anti-Spike (S) IgG1 Fc glycosylation profiles in response to mRNA, adenoviral, and protein-based COVID-19 vaccines by mass spectrometry (MS). All vaccines induced a transient increase of antigen-specific IgG1 Fc galactosylation and sialylation. An initial, transient increase of afucosylated IgG was induced by membrane-encoding S protein formulations. A fucose-sensitive ELISA for antigen-specific IgG (FEASI) exploiting FcγRIIIa affinity for afucosylated IgG was used as an orthogonal method to confirm the LC-MS-based afucosylation readout. Our data suggest that vaccine-induced anti-S IgG glycosylation is dynamic, and although variation is seen between different vaccine platforms and individuals, the evolution of glycosylation patterns display marked overlaps.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
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| Enthalten in: |
iScience - 26(2023), 9 vom: 15. Sept., Seite 107619 |
| Sprache: |
Englisch |
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| Links: |
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| Themen: |
Glycomics |
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| Anmerkungen: |
Date Revised 19.09.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.isci.2023.107619 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM361684541 |
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| 100 | 1 | |a Van Coillie, Julie |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines |
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| 500 | |a Date Revised 19.09.2023 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2023 The Author(s). | ||
| 520 | |a IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both are influenced by the composition of the conserved N-linked glycan located in the IgG Fc domain. Here, we compared the anti-Spike (S) IgG1 Fc glycosylation profiles in response to mRNA, adenoviral, and protein-based COVID-19 vaccines by mass spectrometry (MS). All vaccines induced a transient increase of antigen-specific IgG1 Fc galactosylation and sialylation. An initial, transient increase of afucosylated IgG was induced by membrane-encoding S protein formulations. A fucose-sensitive ELISA for antigen-specific IgG (FEASI) exploiting FcγRIIIa affinity for afucosylated IgG was used as an orthogonal method to confirm the LC-MS-based afucosylation readout. Our data suggest that vaccine-induced anti-S IgG glycosylation is dynamic, and although variation is seen between different vaccine platforms and individuals, the evolution of glycosylation patterns display marked overlaps | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Glycomics | |
| 650 | 4 | |a Immune response | |
| 650 | 4 | |a Immunology | |
| 650 | 4 | |a Microbiology | |
| 700 | 1 | |a Pongracz, Tamas |e verfasserin |4 aut | |
| 700 | 1 | |a Šuštić, Tonći |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Wenjun |e verfasserin |4 aut | |
| 700 | 1 | |a Nouta, Jan |e verfasserin |4 aut | |
| 700 | 1 | |a Le Gars, Mathieu |e verfasserin |4 aut | |
| 700 | 1 | |a Keijzer, Sofie |e verfasserin |4 aut | |
| 700 | 1 | |a Linty, Federica |e verfasserin |4 aut | |
| 700 | 1 | |a Cristianawati, Olvi |e verfasserin |4 aut | |
| 700 | 1 | |a Keijser, Jim B D |e verfasserin |4 aut | |
| 700 | 1 | |a Visser, Remco |e verfasserin |4 aut | |
| 700 | 1 | |a van Vught, Lonneke A |e verfasserin |4 aut | |
| 700 | 1 | |a Slim, Marleen A |e verfasserin |4 aut | |
| 700 | 1 | |a van Mourik, Niels |e verfasserin |4 aut | |
| 700 | 1 | |a Smit, Merel J |e verfasserin |4 aut | |
| 700 | 1 | |a Sander, Adam |e verfasserin |4 aut | |
| 700 | 1 | |a Schmidt, David E |e verfasserin |4 aut | |
| 700 | 1 | |a Steenhuis, Maurice |e verfasserin |4 aut | |
| 700 | 1 | |a Rispens, Theo |e verfasserin |4 aut | |
| 700 | 1 | |a Nielsen, Morten A |e verfasserin |4 aut | |
| 700 | 1 | |a Mordmüller, Benjamin G |e verfasserin |4 aut | |
| 700 | 1 | |a Vlaar, Alexander P J |e verfasserin |4 aut | |
| 700 | 1 | |a Ellen van der Schoot, C |e verfasserin |4 aut | |
| 700 | 1 | |a Roozendaal, Ramon |e verfasserin |4 aut | |
| 700 | 1 | |a Wuhrer, Manfred |e verfasserin |4 aut | |
| 700 | 1 | |a Vidarsson, Gestur |e verfasserin |4 aut | |
| 700 | 0 | |a UMC COVID-19 S3/HCW study group |e verfasserin |4 aut | |
| 700 | 0 | |a Fatebenefratelli-Sacco Infectious Diseases Physicians group |e verfasserin |4 aut | |
| 700 | 0 | |a Radboud University Medical Center (RUMC) and COUGH1 study group |e verfasserin |4 aut | |
| 700 | 1 | |a Appelman, Brent |e investigator |4 oth | |
| 700 | 1 | |a van de Beek, Diederik |e investigator |4 oth | |
| 700 | 1 | |a Bomers, Marije K |e investigator |4 oth | |
| 700 | 1 | |a de Brabander, Justin |e investigator |4 oth | |
| 700 | 1 | |a Brouwer, Matthijs C |e investigator |4 oth | |
| 700 | 1 | |a Buis, David Tp |e investigator |4 oth | |
| 700 | 1 | |a Chekrouni, Nora |e investigator |4 oth | |
| 700 | 1 | |a van Gils, Marit J |e investigator |4 oth | |
| 700 | 1 | |a de Jong, Menno D |e investigator |4 oth | |
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| 700 | 1 | |a Olie, Sabine E |e investigator |4 oth | |
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| 700 | 1 | |a Wiersinga, Joost W |e investigator |4 oth | |
| 700 | 1 | |a Spinello, Antinori |e investigator |4 oth | |
| 700 | 1 | |a Bassoli, Cinzia |e investigator |4 oth | |
| 700 | 1 | |a Bestetti, Giovanna |e investigator |4 oth | |
| 700 | 1 | |a Corbellino, Mario |e investigator |4 oth | |
| 700 | 1 | |a Covizzi, Alice |e investigator |4 oth | |
| 700 | 1 | |a Lupo, Angelica |e investigator |4 oth | |
| 700 | 1 | |a Milazzo, Laura |e investigator |4 oth | |
| 700 | 1 | |a Schiuma, Marco |e investigator |4 oth | |
| 700 | 1 | |a Torre, Alessandro |e investigator |4 oth | |
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| 700 | 1 | |a Esen, Meral |e investigator |4 oth | |
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