Structural changes in perineuronal nets and their perforating GABAergic synapses precede motor coordination recovery post stroke
© 2023. National Science Council of the Republic of China (Taiwan)..
BACKGROUND: Stroke remains one of the leading causes of long-term disability worldwide, and the development of effective restorative therapies is hindered by an incomplete understanding of intrinsic brain recovery mechanisms. Growing evidence indicates that the brain extracellular matrix (ECM) has major implications for neuroplasticity. Here we explored how perineuronal nets (PNNs), the facet-like ECM layers surrounding fast-spiking interneurons, contribute to neurological recovery after focal cerebral ischemia in mice with and without induced stroke tolerance.
METHODS: We investigated the structural remodeling of PNNs after stroke using 3D superresolution stimulated emission depletion (STED) and structured illumination (SR-SIM) microscopy. Superresolution imaging allowed for the precise reconstruction of PNN morphology using graphs, which are mathematical constructs designed for topological analysis. Focal cerebral ischemia was induced by transient occlusion of the middle cerebral artery (tMCAO). PNN-associated synapses and contacts with microglia/macrophages were quantified using high-resolution confocal microscopy.
RESULTS: PNNs undergo transient structural changes after stroke allowing for the dynamic reorganization of GABAergic input to motor cortical L5 interneurons. The coherent remodeling of PNNs and their perforating inhibitory synapses precedes the recovery of motor coordination after stroke and depends on the severity of the ischemic injury. Morphological alterations in PNNs correlate with the increased surface of contact between activated microglia/macrophages and PNN-coated neurons.
CONCLUSIONS: Our data indicate a novel mechanism of post stroke neuroplasticity involving the tripartite interaction between PNNs, synapses, and microglia/macrophages. We propose that prolonging PNN loosening during the post-acute period can extend the opening neuroplasticity window into the chronic stroke phase.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
|---|---|
| Enthalten in: |
Journal of biomedical science - 30(2023), 1 vom: 01. Sept., Seite 76 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Dzyubenko, Egor [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Extracellular matrix |
|---|
| Anmerkungen: |
Date Completed 04.09.2023 Date Revised 22.11.2023 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1186/s12929-023-00971-x |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM361560796 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM361560796 | ||
| 003 | DE-627 | ||
| 005 | 20231226085412.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1186/s12929-023-00971-x |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1205.xml |
| 035 | |a (DE-627)NLM361560796 | ||
| 035 | |a (NLM)37658339 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Dzyubenko, Egor |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Structural changes in perineuronal nets and their perforating GABAergic synapses precede motor coordination recovery post stroke |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 04.09.2023 | ||
| 500 | |a Date Revised 22.11.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. National Science Council of the Republic of China (Taiwan). | ||
| 520 | |a BACKGROUND: Stroke remains one of the leading causes of long-term disability worldwide, and the development of effective restorative therapies is hindered by an incomplete understanding of intrinsic brain recovery mechanisms. Growing evidence indicates that the brain extracellular matrix (ECM) has major implications for neuroplasticity. Here we explored how perineuronal nets (PNNs), the facet-like ECM layers surrounding fast-spiking interneurons, contribute to neurological recovery after focal cerebral ischemia in mice with and without induced stroke tolerance | ||
| 520 | |a METHODS: We investigated the structural remodeling of PNNs after stroke using 3D superresolution stimulated emission depletion (STED) and structured illumination (SR-SIM) microscopy. Superresolution imaging allowed for the precise reconstruction of PNN morphology using graphs, which are mathematical constructs designed for topological analysis. Focal cerebral ischemia was induced by transient occlusion of the middle cerebral artery (tMCAO). PNN-associated synapses and contacts with microglia/macrophages were quantified using high-resolution confocal microscopy | ||
| 520 | |a RESULTS: PNNs undergo transient structural changes after stroke allowing for the dynamic reorganization of GABAergic input to motor cortical L5 interneurons. The coherent remodeling of PNNs and their perforating inhibitory synapses precedes the recovery of motor coordination after stroke and depends on the severity of the ischemic injury. Morphological alterations in PNNs correlate with the increased surface of contact between activated microglia/macrophages and PNN-coated neurons | ||
| 520 | |a CONCLUSIONS: Our data indicate a novel mechanism of post stroke neuroplasticity involving the tripartite interaction between PNNs, synapses, and microglia/macrophages. We propose that prolonging PNN loosening during the post-acute period can extend the opening neuroplasticity window into the chronic stroke phase | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Extracellular matrix | |
| 650 | 4 | |a Fluorescence nanoscopy | |
| 650 | 4 | |a Neuroinflammation | |
| 650 | 4 | |a Parvalbumin interneurons | |
| 650 | 4 | |a Stroke recovery | |
| 650 | 4 | |a Synaptic rewiring | |
| 700 | 1 | |a Willig, Katrin I |e verfasserin |4 aut | |
| 700 | 1 | |a Yin, Dongpei |e verfasserin |4 aut | |
| 700 | 1 | |a Sardari, Maryam |e verfasserin |4 aut | |
| 700 | 1 | |a Tokmak, Erdin |e verfasserin |4 aut | |
| 700 | 1 | |a Labus, Patrick |e verfasserin |4 aut | |
| 700 | 1 | |a Schmermund, Ben |e verfasserin |4 aut | |
| 700 | 1 | |a Hermann, Dirk M |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of biomedical science |d 1994 |g 30(2023), 1 vom: 01. Sept., Seite 76 |w (DE-627)NLM093651961 |x 1423-0127 |7 nnns |
| 773 | 1 | 8 | |g volume:30 |g year:2023 |g number:1 |g day:01 |g month:09 |g pages:76 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1186/s12929-023-00971-x |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 30 |j 2023 |e 1 |b 01 |c 09 |h 76 | ||