Influence of different LED wavelengths on retinal melatonin levels - A rodent study
Copyright © 2023 Elsevier B.V. All rights reserved..
BACKGROUND: Retinal melatonin is crucial for neuroprotection. Exposure to light-emitting diodes (LEDs) affects retinal neurons, possibly influencing retinal melatonin levels. Hence, we aimed to quantify the retinal melatonin level with different LED wavelengths.
METHOD: A total of 24 Sprague Dawley (SD) male rats were divided into four groups (n = 6 in each group) as normal controls (NC), blue light (BL), white light (WL), and yellow light (YL). The rats in the experimental groups were exposed to different wavelengths of LEDs for 28 days (12:12 h light-dark cycle) with uniform illumination of 450-500 lx. Following exposure, the rats were subjected to behavioral tests such as passive avoidance and elevated plus maze tests. Following the behavior tests, the rats were sacrificed, eyes were enucleated, and retinal tissue was stored at -80 °C. The homogenized retina was used for reactive oxygen species (ROS) and melatonin quantification using an enzyme-linked immunosorbent assay (ELISA) kit.
RESULTS: Passive avoidance test revealed a significant difference across the groups (p < 0.0004). The BL exposure group demonstrated increased latency to enter the dark compartment (DC) and impaired motor memory. The elevated plus maze test revealed a significant difference across all the groups (p < 0.012), where the time spent in the closed arm was greater in the BL exposure group. Comparison of ROS levels revealed a significant difference across the groups (p < 0.0001), with increased nitric oxide concentrations in the experimental groups. Melatonin levels were significantly decreased in the light exposure groups (p < 0.0001) compared to the NC group.
CONCLUSION: Cumulative exposure to different LED wavelengths resulted in increased anxiety with impaired motor activity. This was also complemented by the addition of oxidative stress leading to decreased melatonin levels in the retina, which might trigger retinal neuronal damage.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:904 |
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| Enthalten in: |
The Science of the total environment - 904(2023) vom: 15. Dez., Seite 166665 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Joylin, Stelyna [VerfasserIn] |
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| Links: |
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| Themen: |
Blue light |
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| Anmerkungen: |
Date Completed 06.11.2023 Date Revised 06.11.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.scitotenv.2023.166665 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM361501927 |
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| 100 | 1 | |a Joylin, Stelyna |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Influence of different LED wavelengths on retinal melatonin levels - A rodent study |
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| 500 | |a Date Revised 06.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a BACKGROUND: Retinal melatonin is crucial for neuroprotection. Exposure to light-emitting diodes (LEDs) affects retinal neurons, possibly influencing retinal melatonin levels. Hence, we aimed to quantify the retinal melatonin level with different LED wavelengths | ||
| 520 | |a METHOD: A total of 24 Sprague Dawley (SD) male rats were divided into four groups (n = 6 in each group) as normal controls (NC), blue light (BL), white light (WL), and yellow light (YL). The rats in the experimental groups were exposed to different wavelengths of LEDs for 28 days (12:12 h light-dark cycle) with uniform illumination of 450-500 lx. Following exposure, the rats were subjected to behavioral tests such as passive avoidance and elevated plus maze tests. Following the behavior tests, the rats were sacrificed, eyes were enucleated, and retinal tissue was stored at -80 °C. The homogenized retina was used for reactive oxygen species (ROS) and melatonin quantification using an enzyme-linked immunosorbent assay (ELISA) kit | ||
| 520 | |a RESULTS: Passive avoidance test revealed a significant difference across the groups (p < 0.0004). The BL exposure group demonstrated increased latency to enter the dark compartment (DC) and impaired motor memory. The elevated plus maze test revealed a significant difference across all the groups (p < 0.012), where the time spent in the closed arm was greater in the BL exposure group. Comparison of ROS levels revealed a significant difference across the groups (p < 0.0001), with increased nitric oxide concentrations in the experimental groups. Melatonin levels were significantly decreased in the light exposure groups (p < 0.0001) compared to the NC group | ||
| 520 | |a CONCLUSION: Cumulative exposure to different LED wavelengths resulted in increased anxiety with impaired motor activity. This was also complemented by the addition of oxidative stress leading to decreased melatonin levels in the retina, which might trigger retinal neuronal damage | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Blue light | |
| 650 | 4 | |a Circadian cycle | |
| 650 | 4 | |a Light exposure | |
| 650 | 4 | |a Melatonin | |
| 650 | 4 | |a Rats | |
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| 650 | 4 | |a Yellow light | |
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| 700 | 1 | |a Mutalik, Srinivas |e verfasserin |4 aut | |
| 700 | 1 | |a Kalaivani, Manokaran |e verfasserin |4 aut | |
| 700 | 1 | |a Shenoy, Revathi P |e verfasserin |4 aut | |
| 700 | 1 | |a Ghosh, Mousumi |e verfasserin |4 aut | |
| 700 | 1 | |a Nishitha |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar, Elizebeth Olive Akansha Manoj |e verfasserin |4 aut | |
| 700 | 1 | |a Theruveethi, Nagarajan |e verfasserin |4 aut | |
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