hsa-miR-181-5p inhibits human immunodeficiency virus type 1 replication by downregulating DDX3X expression
Copyright © 2023 Elsevier Inc. All rights reserved..
BACKGROUND: HIV-1 infection affects expression profiles of microRNA. miR-181 is found negatively correlated with HIV-1 viral load. This study aimed to explain that miR-181 targets DDX3X, a host factor involved in HIV-1 nuclear export, thereby inhibiting HIV-1 replication.
METHODS: To verify our hypothesis, first, the relationship between miR-181 expression, DDX3X expression, and HIV-1 viral load was analyzed. Second, miR-181 mimics were transfected into Jurkat cells infected with wild pNL4-3 strain or H9-IIIB cells with HIV-1 replication-competent for HIV-1 viral protein P24(Gag) detection. Besides the reporter gene plasmid containing the DDX3X mRNA sequence was transfected into 293T cells to demonstrate the targeting of miR-181 to the DDX3X mRNA. Finally, the spliced, unspliced, or incompletely spliced HIV-1 transcripts and HIV-1 Tat, Rev, and Gag mRNA were also detected after miR-181 transfection.
RESULTS: Our result proved that miR-181 significantly reduced the HIV-1 viral protein Gag(P24) level and targeted DDX3X mRNA 3'-UTR, inhibiting the unspliced or incompletely spliced HIV-1 mRNA's nuclear export.
CONCLUSION: Our results confirmed that miR-181 is involved in HIV-1 viral replication in lymphocytes by downregulating DDX3X expression. The research provides a research basis for future HIV-1 antiviral research.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:587 |
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| Enthalten in: |
Virology - 587(2023) vom: 15. Okt., Seite 109868 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Han, Dating [VerfasserIn] |
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Date Revised 05.10.2023 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1016/j.virol.2023.109868 |
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| funding: |
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NLM361497083 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a Copyright © 2023 Elsevier Inc. All rights reserved. | ||
| 520 | |a BACKGROUND: HIV-1 infection affects expression profiles of microRNA. miR-181 is found negatively correlated with HIV-1 viral load. This study aimed to explain that miR-181 targets DDX3X, a host factor involved in HIV-1 nuclear export, thereby inhibiting HIV-1 replication | ||
| 520 | |a METHODS: To verify our hypothesis, first, the relationship between miR-181 expression, DDX3X expression, and HIV-1 viral load was analyzed. Second, miR-181 mimics were transfected into Jurkat cells infected with wild pNL4-3 strain or H9-IIIB cells with HIV-1 replication-competent for HIV-1 viral protein P24(Gag) detection. Besides the reporter gene plasmid containing the DDX3X mRNA sequence was transfected into 293T cells to demonstrate the targeting of miR-181 to the DDX3X mRNA. Finally, the spliced, unspliced, or incompletely spliced HIV-1 transcripts and HIV-1 Tat, Rev, and Gag mRNA were also detected after miR-181 transfection | ||
| 520 | |a RESULTS: Our result proved that miR-181 significantly reduced the HIV-1 viral protein Gag(P24) level and targeted DDX3X mRNA 3'-UTR, inhibiting the unspliced or incompletely spliced HIV-1 mRNA's nuclear export | ||
| 520 | |a CONCLUSION: Our results confirmed that miR-181 is involved in HIV-1 viral replication in lymphocytes by downregulating DDX3X expression. The research provides a research basis for future HIV-1 antiviral research | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a DDX3X | |
| 650 | 4 | |a HIV-1 replication | |
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| 700 | 1 | |a Yin, Wanpeng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiaodi |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Xiangyun |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Nanping |e verfasserin |4 aut | |
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