Immulina as an Immunostimulatory Supplement : Formulation and Pharmacological Studies
Thieme. All rights reserved..
Immulina is a commercially available extract of Arthrospira platensis enriched with bacterial lipoproteins that acts as a potent Toll-like receptor 2 agonist. However, the immunostimulatory effect of Immulina is not well understood in vivo. Here, to devise an Immulina formulation suitable for in vivo oral gavage dosing, Immulina nanosuspension was prepared and freeze-dried to yield lyophilized nano-Immulina, which had an average particle size of around 300 nm and fully retained the bioactivity as a Toll-like receptor 2 agonist. Compared to the regular Immulina powder, lyophilized nano-Immulina notably accelerated the dissolution in aqueous media. Immulina nanosuspension was found to stimulate the production of proinflammatory cytokines in murine bone marrow-derived dendritic cells and macrophages. The immune response to Immulina was investigated in healthy mice by longitudinally monitoring the phagocytic activity of circulating neutrophils as a surrogate marker. Following daily oral ingestion of Immulina nanosuspension (10 mg/mouse/day), the phagocytic activity of circulating neutrophils was significantly elevated, suggesting an important mechanism for Immulina to enhance innate immunity.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2023 |
---|---|
Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:89 |
---|---|
Enthalten in: |
Planta medica - 89(2023), 15 vom: 30. Dez., Seite 1483-1492 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Li, Yusheng [VerfasserIn] |
---|
Links: |
---|
Themen: |
Adjuvants, Immunologic |
---|
Anmerkungen: |
Date Completed 30.11.2023 Date Revised 05.04.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1055/a-2156-4653 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM361457782 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM361457782 | ||
003 | DE-627 | ||
005 | 20240405233114.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2023 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1055/a-2156-4653 |2 doi | |
028 | 5 | 2 | |a pubmed24n1366.xml |
035 | |a (DE-627)NLM361457782 | ||
035 | |a (NLM)37647915 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Li, Yusheng |e verfasserin |4 aut | |
245 | 1 | 0 | |a Immulina as an Immunostimulatory Supplement |b Formulation and Pharmacological Studies |
264 | 1 | |c 2023 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 30.11.2023 | ||
500 | |a Date Revised 05.04.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Thieme. All rights reserved. | ||
520 | |a Immulina is a commercially available extract of Arthrospira platensis enriched with bacterial lipoproteins that acts as a potent Toll-like receptor 2 agonist. However, the immunostimulatory effect of Immulina is not well understood in vivo. Here, to devise an Immulina formulation suitable for in vivo oral gavage dosing, Immulina nanosuspension was prepared and freeze-dried to yield lyophilized nano-Immulina, which had an average particle size of around 300 nm and fully retained the bioactivity as a Toll-like receptor 2 agonist. Compared to the regular Immulina powder, lyophilized nano-Immulina notably accelerated the dissolution in aqueous media. Immulina nanosuspension was found to stimulate the production of proinflammatory cytokines in murine bone marrow-derived dendritic cells and macrophages. The immune response to Immulina was investigated in healthy mice by longitudinally monitoring the phagocytic activity of circulating neutrophils as a surrogate marker. Following daily oral ingestion of Immulina nanosuspension (10 mg/mouse/day), the phagocytic activity of circulating neutrophils was significantly elevated, suggesting an important mechanism for Immulina to enhance innate immunity | ||
650 | 4 | |a Journal Article | |
650 | 7 | |a Immulina |2 NLM | |
650 | 7 | |a Toll-Like Receptor 2 |2 NLM | |
650 | 7 | |a Polysaccharides, Bacterial |2 NLM | |
650 | 7 | |a Adjuvants, Immunologic |2 NLM | |
700 | 1 | |a Ji, Nan |e verfasserin |4 aut | |
700 | 1 | |a Wang, Minia |e verfasserin |4 aut | |
700 | 1 | |a Pugh, Nirmal D |e verfasserin |4 aut | |
700 | 1 | |a Khan, Ikhlas A |e verfasserin |4 aut | |
700 | 1 | |a Tan, Chalet |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Planta medica |d 1961 |g 89(2023), 15 vom: 30. Dez., Seite 1483-1492 |w (DE-627)NLM000844535 |x 1439-0221 |7 nnns |
773 | 1 | 8 | |g volume:89 |g year:2023 |g number:15 |g day:30 |g month:12 |g pages:1483-1492 |
856 | 4 | 0 | |u http://dx.doi.org/10.1055/a-2156-4653 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 89 |j 2023 |e 15 |b 30 |c 12 |h 1483-1492 |