Platelet extracellular vesicles and their mitochondrial content improve the mitochondrial bioenergetics of cellular immune recipients
© 2023 The Authors. Transfusion published by Wiley Periodicals LLC on behalf of AABB..
BACKGROUND: Mitochondria play a critical role in the production of cell energy and the regulation of cell death. Therefore, mitochondria orchestrate numerous cell effector functions, including fine-tuning the immune system. While mitochondria are mainly found intracellularly, they can escape the confine of the cell during the process of extracellular vesicle release. Platelets patrol blood vessels to ensure vasculature integrity and to support the immune system. In blood, platelets are the primary source of circulating mitochondria. Activated platelets produce extracellular vesicles, including a subset of mitochondria-containing vesicles.
STUDY DESIGN AND METHODS: We characterized mitochondrial functions in platelet-derived extracellular vesicles, and examined whether they could impact the bioenergetics of cellular immune recipients using an extracellular flux analyzer to measure real-time bioenergetics.
RESULTS: We validated that extracellular vesicles derived from activated platelets contain the necessary mitochondrial machinery to respirate and generate energy. Moreover, neutrophils and monocytes efficiently captured platelet-derived extracellular vesicles, enhancing their mitochondrial fitness. This process required functional mitochondria from donor platelets, as it was abolished by the inactivation of extracellular mitochondria using mitochondrial poison.
DISCUSSION: Together, the data suggest that extracellular mitochondria produced by platelets may support other metabolic functions through transcellular bioenergetics.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:63 |
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| Enthalten in: |
Transfusion - 63(2023), 10 vom: 01. Okt., Seite 1983-1996 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Pelletier, Martin [VerfasserIn] |
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| Links: |
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| Themen: |
Hematology - platelets |
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| Anmerkungen: |
Date Completed 05.10.2023 Date Revised 09.10.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/trf.17524 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM361402597 |
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| 100 | 1 | |a Pelletier, Martin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Platelet extracellular vesicles and their mitochondrial content improve the mitochondrial bioenergetics of cellular immune recipients |
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| 500 | |a Date Completed 05.10.2023 | ||
| 500 | |a Date Revised 09.10.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 The Authors. Transfusion published by Wiley Periodicals LLC on behalf of AABB. | ||
| 520 | |a BACKGROUND: Mitochondria play a critical role in the production of cell energy and the regulation of cell death. Therefore, mitochondria orchestrate numerous cell effector functions, including fine-tuning the immune system. While mitochondria are mainly found intracellularly, they can escape the confine of the cell during the process of extracellular vesicle release. Platelets patrol blood vessels to ensure vasculature integrity and to support the immune system. In blood, platelets are the primary source of circulating mitochondria. Activated platelets produce extracellular vesicles, including a subset of mitochondria-containing vesicles | ||
| 520 | |a STUDY DESIGN AND METHODS: We characterized mitochondrial functions in platelet-derived extracellular vesicles, and examined whether they could impact the bioenergetics of cellular immune recipients using an extracellular flux analyzer to measure real-time bioenergetics | ||
| 520 | |a RESULTS: We validated that extracellular vesicles derived from activated platelets contain the necessary mitochondrial machinery to respirate and generate energy. Moreover, neutrophils and monocytes efficiently captured platelet-derived extracellular vesicles, enhancing their mitochondrial fitness. This process required functional mitochondria from donor platelets, as it was abolished by the inactivation of extracellular mitochondria using mitochondrial poison | ||
| 520 | |a DISCUSSION: Together, the data suggest that extracellular mitochondria produced by platelets may support other metabolic functions through transcellular bioenergetics | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a hematology - platelets | |
| 650 | 4 | |a immunology (other than RBC serology) | |
| 650 | 4 | |a molecular biology | |
| 700 | 1 | |a Breton, Yann |e verfasserin |4 aut | |
| 700 | 1 | |a Allaeys, Isabelle |e verfasserin |4 aut | |
| 700 | 1 | |a Becker, Yann |e verfasserin |4 aut | |
| 700 | 1 | |a Benson, Tom |e verfasserin |4 aut | |
| 700 | 1 | |a Boilard, Eric |e verfasserin |4 aut | |
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