Partial desensitization of MYC2 transcription factor alters the interaction with jasmonate signaling components and affects specialized metabolism
Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved..
The activity of bHLH transcription factor MYC2, a key regulator in jasmonate signaling and plant specialized metabolism, is sensitive to repression by JASMONATE-ZIM-domain (JAZ) proteins and co-activation by the mediator subunit MED25. The substitution of a conserved aspartic acid (D) to asparagine (N) in the JAZ-interacting domain (JID) of Arabidopsis MYC2 affects interaction with JAZ, although the mechanism remained unclear. The effects of the conserved residue MYC2D128 on interaction with MED25 have not been investigated. Using tobacco as a model, we generated all possible substitutions of aspartic acid 128 (D128) in NtMYC2a. NtMYC2aD128N partially desensitized the repression by JAZ proteins, while strongly interacting with MED25, resulting in increased expression of nicotine pathway genes and nicotine accumulation in tobacco hairy roots overexpressing NtMYC2aD128N compared to those overexpressing NtMYC2a. The proline substitution, NtMYC2aD128P, negatively affected transactivation and abolished the interaction with JAZ proteins and MED25. Structural modeling and simulation suggest that the overall stability of the JID binding pocket is a predominant cause for the observed effects of substitutions at D128. The D128N substitution has an overall stabilizing effect on the binding pocket, which is destabilized by D128P. Our study offers an innovative tool to increase the production of plant natural products.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:252 |
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| Enthalten in: |
International journal of biological macromolecules - 252(2023) vom: 01. Dez., Seite 126472 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hou, Xin [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 15.11.2023 Date Revised 15.11.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijbiomac.2023.126472 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM361239521 |
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| 100 | 1 | |a Hou, Xin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Partial desensitization of MYC2 transcription factor alters the interaction with jasmonate signaling components and affects specialized metabolism |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Revised 15.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a The activity of bHLH transcription factor MYC2, a key regulator in jasmonate signaling and plant specialized metabolism, is sensitive to repression by JASMONATE-ZIM-domain (JAZ) proteins and co-activation by the mediator subunit MED25. The substitution of a conserved aspartic acid (D) to asparagine (N) in the JAZ-interacting domain (JID) of Arabidopsis MYC2 affects interaction with JAZ, although the mechanism remained unclear. The effects of the conserved residue MYC2D128 on interaction with MED25 have not been investigated. Using tobacco as a model, we generated all possible substitutions of aspartic acid 128 (D128) in NtMYC2a. NtMYC2aD128N partially desensitized the repression by JAZ proteins, while strongly interacting with MED25, resulting in increased expression of nicotine pathway genes and nicotine accumulation in tobacco hairy roots overexpressing NtMYC2aD128N compared to those overexpressing NtMYC2a. The proline substitution, NtMYC2aD128P, negatively affected transactivation and abolished the interaction with JAZ proteins and MED25. Structural modeling and simulation suggest that the overall stability of the JID binding pocket is a predominant cause for the observed effects of substitutions at D128. The D128N substitution has an overall stabilizing effect on the binding pocket, which is destabilized by D128P. Our study offers an innovative tool to increase the production of plant natural products | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Homology structural modeling | |
| 650 | 4 | |a MEDIATOR25 | |
| 650 | 4 | |a MYC2 | |
| 650 | 4 | |a Protein-protein interaction | |
| 650 | 4 | |a Specialized metabolism | |
| 650 | 4 | |a Transcriptional regulation | |
| 650 | 7 | |a Transcription Factors |2 NLM | |
| 650 | 7 | |a jasmonic acid |2 NLM | |
| 650 | 7 | |a 6RI5N05OWW |2 NLM | |
| 650 | 7 | |a Arabidopsis Proteins |2 NLM | |
| 650 | 7 | |a Nicotine |2 NLM | |
| 650 | 7 | |a 6M3C89ZY6R |2 NLM | |
| 650 | 7 | |a Aspartic Acid |2 NLM | |
| 650 | 7 | |a 30KYC7MIAI |2 NLM | |
| 650 | 7 | |a MYC2 protein, Arabidopsis |2 NLM | |
| 650 | 7 | |a Basic Helix-Loop-Helix Leucine Zipper Transcription Factors |2 NLM | |
| 700 | 1 | |a Singh, Sanjay Kumar |e verfasserin |4 aut | |
| 700 | 1 | |a Werkman, Joshua R |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yongliang |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Qinghua |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Xia |e verfasserin |4 aut | |
| 700 | 1 | |a Patra, Barunava |e verfasserin |4 aut | |
| 700 | 1 | |a Sui, Xueyi |e verfasserin |4 aut | |
| 700 | 1 | |a Lyu, Ruiqing |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Bingwu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaoyu |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yongqing |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Pattanaik, Sitakanta |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Ling |e verfasserin |4 aut | |
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