Cleavage Under Targets & Release Using Nuclease (CUT&RUN) of Histone Modifications in Epidermal Stem Cells of Adult Murine Skin
© 2023. Springer Science+Business Media, LLC..
Cleavage Under Targets & Release Using Nuclease (CUT&RUN) has swiftly become the preferred procedure over the past few years for genomic mapping and detecting interactions between chromatin and its bound proteins. CUT&RUN is now being widely used for characterizing the epigenetic landscape in many cell types as it utilizes far less cell numbers when compared to Chromatin Immunoprecipitation-sequencing (ChIP-seq), thereby making it a powerful tool for researchers working with limited material. This protocol has been specifically optimized for detecting histone modifications in fluorescence-activated cell sorting (FACS)-isolated epidermal stem cells from adult mice.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2736 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2736(2024) vom: 05., Seite 9-21 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Flora, Pooja [VerfasserIn] |
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| Links: |
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| Themen: |
CUT&RUN |
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| Anmerkungen: |
Date Completed 08.11.2023 Date Revised 06.02.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/7651_2023_499 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Cleavage Under Targets & Release Using Nuclease (CUT&RUN) has swiftly become the preferred procedure over the past few years for genomic mapping and detecting interactions between chromatin and its bound proteins. CUT&RUN is now being widely used for characterizing the epigenetic landscape in many cell types as it utilizes far less cell numbers when compared to Chromatin Immunoprecipitation-sequencing (ChIP-seq), thereby making it a powerful tool for researchers working with limited material. This protocol has been specifically optimized for detecting histone modifications in fluorescence-activated cell sorting (FACS)-isolated epidermal stem cells from adult mice | ||
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