The application of electrosprayed minocycline-loaded PLGA microparticles for the treatment of glioblastoma
© 2023 Wiley Periodicals LLC..
The survival of patients with glioblastoma multiforme (GBM), the most common and invasive form of malignant brain tumors, remains poor despite advances in current treatment methods including surgery, radiotherapy, and chemotherapy. Minocycline is a semi-synthetic tetracycline derivative that has been widely used as an antibiotic and more recently, it has been utilized as an antiangiogenic factor to inhibit tumorigenesis. The objective of this study was to investigate the utilization of electrospraying process to fabricate minocycline-loaded poly(lactic-co-glycolic acid) (PLGA) microparticles with high drug loading and loading efficiency and to evaluate their ability to induce cell toxicity in human glioblastoma (i.e., U87-MG) cells. The results from this study demonstrated that solvent mixture of dicholoromethane (DCM) and methanol is the optimal solvent combination for minocycline and larger amount of methanol (i.e., 70:30) resulted in a higher drug loading. All three solvent ratios of DCM:methanol tested produced microparticles that were both spherical and smooth, all in the micron size range. The electrosprayed microparticles were able to elicit a cytotoxic response in U87-MG glioblastoma cells at a lower concentration of drug compared to the free drug. This work provides proof of concept to the hypothesis that electrosprayed minocycline-loaded PLGA microparticles can be a promising agent for the treatment of GBM and could have potential application for cancer therapies.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:120 |
|---|---|
| Enthalten in: |
Biotechnology and bioengineering - 120(2023), 11 vom: 13. Nov., Seite 3409-3422 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Arriaga, Marco A [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Drug delivery |
|---|
| Anmerkungen: |
Date Revised 24.10.2023 published: Print-Electronic Citation Status Publisher |
|---|
| doi: |
10.1002/bit.28527 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM361041985 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM361041985 | ||
| 003 | DE-627 | ||
| 005 | 20231226084316.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/bit.28527 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1203.xml |
| 035 | |a (DE-627)NLM361041985 | ||
| 035 | |a (NLM)37605630 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Arriaga, Marco A |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The application of electrosprayed minocycline-loaded PLGA microparticles for the treatment of glioblastoma |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 24.10.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a © 2023 Wiley Periodicals LLC. | ||
| 520 | |a The survival of patients with glioblastoma multiforme (GBM), the most common and invasive form of malignant brain tumors, remains poor despite advances in current treatment methods including surgery, radiotherapy, and chemotherapy. Minocycline is a semi-synthetic tetracycline derivative that has been widely used as an antibiotic and more recently, it has been utilized as an antiangiogenic factor to inhibit tumorigenesis. The objective of this study was to investigate the utilization of electrospraying process to fabricate minocycline-loaded poly(lactic-co-glycolic acid) (PLGA) microparticles with high drug loading and loading efficiency and to evaluate their ability to induce cell toxicity in human glioblastoma (i.e., U87-MG) cells. The results from this study demonstrated that solvent mixture of dicholoromethane (DCM) and methanol is the optimal solvent combination for minocycline and larger amount of methanol (i.e., 70:30) resulted in a higher drug loading. All three solvent ratios of DCM:methanol tested produced microparticles that were both spherical and smooth, all in the micron size range. The electrosprayed microparticles were able to elicit a cytotoxic response in U87-MG glioblastoma cells at a lower concentration of drug compared to the free drug. This work provides proof of concept to the hypothesis that electrosprayed minocycline-loaded PLGA microparticles can be a promising agent for the treatment of GBM and could have potential application for cancer therapies | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a PLGA microparticles | |
| 650 | 4 | |a drug delivery | |
| 650 | 4 | |a electrospraying | |
| 650 | 4 | |a glioblastoma | |
| 650 | 4 | |a minocycline | |
| 700 | 1 | |a Amieva, Juan A |c Jr |e verfasserin |4 aut | |
| 700 | 1 | |a Quintanilla, Jaqueline |e verfasserin |4 aut | |
| 700 | 1 | |a Jimenez, Angela |e verfasserin |4 aut | |
| 700 | 1 | |a Ledezma, Julio |e verfasserin |4 aut | |
| 700 | 1 | |a Lopez, Silverio |e verfasserin |4 aut | |
| 700 | 1 | |a Martirosyan, Karen S |e verfasserin |4 aut | |
| 700 | 1 | |a Chew, Sue Anne |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biotechnology and bioengineering |d 1976 |g 120(2023), 11 vom: 13. Nov., Seite 3409-3422 |w (DE-627)NLM000014702 |x 1097-0290 |7 nnns |
| 773 | 1 | 8 | |g volume:120 |g year:2023 |g number:11 |g day:13 |g month:11 |g pages:3409-3422 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/bit.28527 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 120 |j 2023 |e 11 |b 13 |c 11 |h 3409-3422 | ||