Biodegradable piezoelectric skin-wound scaffold
Copyright © 2023 Elsevier Ltd. All rights reserved..
Electrical stimulation (ES) induces wound healing and skin regeneration. Combining ES with the tissue-engineering approach, which relies on biomaterials to construct a replacement tissue graft, could offer a self-stimulated scaffold to heal skin-wounds without using potentially toxic growth factors and exogenous cells. Unfortunately, current ES technologies are either ineffective (external stimulations) or unsafe (implanted electrical devices using toxic batteries). Hence, we propose a novel wound-healing strategy that integrates ES with tissue engineering techniques by utilizing a biodegradable self-charged piezoelectric PLLA (Poly (l-lactic acid)) nanofiber matrix. This unique, safe, and stable piezoelectric scaffold can be activated by an external ultrasound (US) to produce well-controlled surface-charges with different polarities, thus serving multiple functions to suppress bacterial growth (negative surface charge) and promote skin regeneration (positive surface charge) at the same time. We demonstrate that the scaffold activated by low intensity/low frequency US can facilitate the proliferation of fibroblast/epithelial cells, enhance expression of genes (collagen I, III, and fibronectin) typical for the wound healing process, and suppress the growth of S. aureus and P. aeruginosa bacteria in vitro simultaneously. This approach induces rapid skin regeneration in a critical-sized skin wound mouse model in vivo. The piezoelectric PLLA skin scaffold thus assumes the role of a multi-tasking, biodegradable, battery-free electrical stimulator which is important for skin-wound healing and bacterial infection prevention simultaneuosly.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:301 |
|---|---|
| Enthalten in: |
Biomaterials - 301(2023) vom: 15. Okt., Seite 122270 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Das, Ritopa [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Biocompatible Materials |
|---|
| Anmerkungen: |
Date Completed 06.09.2023 Date Revised 03.10.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.biomaterials.2023.122270 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM360900755 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM360900755 | ||
| 003 | DE-627 | ||
| 005 | 20231226084012.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.biomaterials.2023.122270 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1202.xml |
| 035 | |a (DE-627)NLM360900755 | ||
| 035 | |a (NLM)37591188 | ||
| 035 | |a (PII)S0142-9612(23)00278-8 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Das, Ritopa |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Biodegradable piezoelectric skin-wound scaffold |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 06.09.2023 | ||
| 500 | |a Date Revised 03.10.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Electrical stimulation (ES) induces wound healing and skin regeneration. Combining ES with the tissue-engineering approach, which relies on biomaterials to construct a replacement tissue graft, could offer a self-stimulated scaffold to heal skin-wounds without using potentially toxic growth factors and exogenous cells. Unfortunately, current ES technologies are either ineffective (external stimulations) or unsafe (implanted electrical devices using toxic batteries). Hence, we propose a novel wound-healing strategy that integrates ES with tissue engineering techniques by utilizing a biodegradable self-charged piezoelectric PLLA (Poly (l-lactic acid)) nanofiber matrix. This unique, safe, and stable piezoelectric scaffold can be activated by an external ultrasound (US) to produce well-controlled surface-charges with different polarities, thus serving multiple functions to suppress bacterial growth (negative surface charge) and promote skin regeneration (positive surface charge) at the same time. We demonstrate that the scaffold activated by low intensity/low frequency US can facilitate the proliferation of fibroblast/epithelial cells, enhance expression of genes (collagen I, III, and fibronectin) typical for the wound healing process, and suppress the growth of S. aureus and P. aeruginosa bacteria in vitro simultaneously. This approach induces rapid skin regeneration in a critical-sized skin wound mouse model in vivo. The piezoelectric PLLA skin scaffold thus assumes the role of a multi-tasking, biodegradable, battery-free electrical stimulator which is important for skin-wound healing and bacterial infection prevention simultaneuosly | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Biodegradable piezoelectric nanofibers | |
| 650 | 4 | |a Electrical stimulation | |
| 650 | 4 | |a Skin regeneration | |
| 650 | 4 | |a Ultrasound | |
| 650 | 4 | |a Wound healing | |
| 650 | 7 | |a Biocompatible Materials |2 NLM | |
| 650 | 7 | |a Collagen Type I |2 NLM | |
| 700 | 1 | |a Le, Thinh T |e verfasserin |4 aut | |
| 700 | 1 | |a Schiff, Benjamin |e verfasserin |4 aut | |
| 700 | 1 | |a Chorsi, Meysam T |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Jinyoung |e verfasserin |4 aut | |
| 700 | 1 | |a Lam, Priscilla |e verfasserin |4 aut | |
| 700 | 1 | |a Kemerley, Andrew |e verfasserin |4 aut | |
| 700 | 1 | |a Supran, Ajayan Mannoor |e verfasserin |4 aut | |
| 700 | 1 | |a Eshed, Amit |e verfasserin |4 aut | |
| 700 | 1 | |a Luu, Ngoc |e verfasserin |4 aut | |
| 700 | 1 | |a Menon, Nikhil G |e verfasserin |4 aut | |
| 700 | 1 | |a Schmidt, Tannin A |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Hanzhang |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Qian |e verfasserin |4 aut | |
| 700 | 1 | |a Thirunavukkarasu, Mahesh |e verfasserin |4 aut | |
| 700 | 1 | |a Maulik, Nilanjana |e verfasserin |4 aut | |
| 700 | 1 | |a Nguyen, Thanh D |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biomaterials |d 1989 |g 301(2023) vom: 15. Okt., Seite 122270 |w (DE-627)NLM012732516 |x 1878-5905 |7 nnns |
| 773 | 1 | 8 | |g volume:301 |g year:2023 |g day:15 |g month:10 |g pages:122270 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.biomaterials.2023.122270 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 301 |j 2023 |b 15 |c 10 |h 122270 | ||