Rolipram-loaded PgP nanoparticle reduces secondary injury and enhances motor function recovery in a rat moderate contusion SCI model
Copyright © 2023. Published by Elsevier Inc..
Spinal cord injury (SCI) results in immediate axonal damage and cell death, as well as a prolonged secondary injury consist of a cascade of pathophysiological processes. One important aspect of secondary injury is activation of phosphodiesterase 4 (PDE4) that leads to reduce cAMP levels in the injured spinal cord. We have developed an amphiphilic copolymer, poly (lactide-co-glycolide)-graft-polyethylenimine (PgP) that can deliver Rolipram, the PDE4 inhibitor. The objective of this work was to investigate the effect of rolipram loaded PgP (Rm-PgP) on secondary injury and motor functional recovery in a rat moderate contusion SCI model. We observed that Rm-PgP can increase cAMP level at the lesion site, and reduce secondary injury such as the inflammatory response by macrophages/microglia, astrogliosis by activated astrocytes and apoptosis as well as improve neuronal survival at 4 weeks post-injury (WPI). We also observed that Rm-PgP can improve motor functional recovery after SCI over 4 WPI.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:53 |
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| Enthalten in: |
Nanomedicine : nanotechnology, biology, and medicine - 53(2023) vom: 15. Sept., Seite 102702 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gao, Jun [VerfasserIn] |
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| Links: |
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| Themen: |
CAMP |
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| Anmerkungen: |
Date Completed 25.09.2023 Date Revised 27.09.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.nano.2023.102702 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2023. Published by Elsevier Inc. | ||
| 520 | |a Spinal cord injury (SCI) results in immediate axonal damage and cell death, as well as a prolonged secondary injury consist of a cascade of pathophysiological processes. One important aspect of secondary injury is activation of phosphodiesterase 4 (PDE4) that leads to reduce cAMP levels in the injured spinal cord. We have developed an amphiphilic copolymer, poly (lactide-co-glycolide)-graft-polyethylenimine (PgP) that can deliver Rolipram, the PDE4 inhibitor. The objective of this work was to investigate the effect of rolipram loaded PgP (Rm-PgP) on secondary injury and motor functional recovery in a rat moderate contusion SCI model. We observed that Rm-PgP can increase cAMP level at the lesion site, and reduce secondary injury such as the inflammatory response by macrophages/microglia, astrogliosis by activated astrocytes and apoptosis as well as improve neuronal survival at 4 weeks post-injury (WPI). We also observed that Rm-PgP can improve motor functional recovery after SCI over 4 WPI | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Motor function | |
| 650 | 4 | |a Neuropathic pain | |
| 650 | 4 | |a PgP nanocarrier | |
| 650 | 4 | |a Rolipram | |
| 650 | 4 | |a Secondary injury | |
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| 700 | 1 | |a Liao, Zhen |e verfasserin |4 aut | |
| 700 | 1 | |a Webb, Ken |e verfasserin |4 aut | |
| 700 | 1 | |a Detloff, Megan Ryan |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Jeoung Soo |e verfasserin |4 aut | |
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