Epigenetic profiling of the D4Z4 locus : Optimization of the protocol for studying DNA methylation at single CpG site level
© 2023 The Authors. Electrophoresis published by Wiley-VCH GmbH..
The alteration of epigenetic modifications, including DNA methylation, can contribute to the etiopathogenesis and progression of many diseases. Among them, facioscapulohumeral dystrophy (FSHD) is a muscular disorder characterized by the loss of repressive epigenetic features affecting the D4Z4 locus (4q35). As a consequence, these alterations are responsible for DNA hypomethylation and a transcriptional-active chromatin conformation change that, in turn, lead to the aberrant expression of DUX4 in muscle cells. In the present study, methylation levels of 29 CpG sites of the DR1 region (within each repeat unit of the D4Z4 macrosatellite) were assessed on 335 subjects by employing primers designed for enhancing the performance of the assay. First, the DR1 original primers were optimized by adding M13 oligonucleotide tails. Moreover, the DR1 reverse primer was replaced with a degenerate one. As a result, the protocol optimization allowed a better sequencing resolution and a more accurate evaluation of DR1 methylation levels. Moreover, the assessment of the repeatability of measurements proved the reliability and robustness of the assay. The optimized protocol emerges as an excellent method to detect methylation levels compatible with FSHD.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
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| Enthalten in: |
Electrophoresis - 44(2023), 19-20 vom: 11. Okt., Seite 1588-1594 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Megalizzi, Domenica [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 12.10.2023 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1002/elps.202300058 |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Megalizzi, Domenica |e verfasserin |4 aut | |
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| 520 | |a © 2023 The Authors. Electrophoresis published by Wiley-VCH GmbH. | ||
| 520 | |a The alteration of epigenetic modifications, including DNA methylation, can contribute to the etiopathogenesis and progression of many diseases. Among them, facioscapulohumeral dystrophy (FSHD) is a muscular disorder characterized by the loss of repressive epigenetic features affecting the D4Z4 locus (4q35). As a consequence, these alterations are responsible for DNA hypomethylation and a transcriptional-active chromatin conformation change that, in turn, lead to the aberrant expression of DUX4 in muscle cells. In the present study, methylation levels of 29 CpG sites of the DR1 region (within each repeat unit of the D4Z4 macrosatellite) were assessed on 335 subjects by employing primers designed for enhancing the performance of the assay. First, the DR1 original primers were optimized by adding M13 oligonucleotide tails. Moreover, the DR1 reverse primer was replaced with a degenerate one. As a result, the protocol optimization allowed a better sequencing resolution and a more accurate evaluation of DR1 methylation levels. Moreover, the assessment of the repeatability of measurements proved the reliability and robustness of the assay. The optimized protocol emerges as an excellent method to detect methylation levels compatible with FSHD | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a D4Z4 | |
| 650 | 4 | |a DNA methylation | |
| 650 | 4 | |a DR1 | |
| 650 | 4 | |a FSHD | |
| 650 | 4 | |a epigenetics | |
| 700 | 1 | |a Trastulli, Giulia |e verfasserin |4 aut | |
| 700 | 1 | |a Caputo, Valerio |e verfasserin |4 aut | |
| 700 | 1 | |a Colantoni, Luca |e verfasserin |4 aut | |
| 700 | 1 | |a Caltagirone, Carlo |e verfasserin |4 aut | |
| 700 | 1 | |a Strafella, Claudia |e verfasserin |4 aut | |
| 700 | 1 | |a Cascella, Raffaella |e verfasserin |4 aut | |
| 700 | 1 | |a Giardina, Emiliano |e verfasserin |4 aut | |
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