Cell Granularity Reflects Immune Cell Function and Enables Selection of Lymphocytes with Superior Attributes for Immunotherapy
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH..
In keeping with the rule of "form follows function", morphological aspects of a cell can reflect its role. Here, it is shown that the cellular granularity of a lymphocyte, represented by its intrinsic side scatter (SSC), is a potent indicator of its cell state and function. The granularity of a lymphocyte increases from naïve to terminal effector state. High-throughput cell-sorting yields a SSChigh population that can mediate immediate effector functions, and a highly prolific SSClow population that can give rise to the replenishment of the memory pool. CAR-T cells derived from the younger SSClow population possess desirable attributes for immunotherapy, manifested by increased naïve-like cells and stem cell memory (TSCM )-like cells together with a balanced CD4/CD8 ratio, as well as enhanced target-killing in vitro and in vivo. Altogether, lymphocyte segregation based on biophysical properties is an effective approach for label-free selection of cells that share collective functions and can have important applications for cell-based immunotherapies.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
Advanced science (Weinheim, Baden-Wurttemberg, Germany) - 10(2023), 28 vom: 02. Okt., Seite e2302175 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wu, Tongjin [VerfasserIn] |
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| Links: |
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| Themen: |
Cell granularity |
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| Anmerkungen: |
Date Completed 09.10.2023 Date Revised 18.10.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/advs.202302175 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH. | ||
| 520 | |a In keeping with the rule of "form follows function", morphological aspects of a cell can reflect its role. Here, it is shown that the cellular granularity of a lymphocyte, represented by its intrinsic side scatter (SSC), is a potent indicator of its cell state and function. The granularity of a lymphocyte increases from naïve to terminal effector state. High-throughput cell-sorting yields a SSChigh population that can mediate immediate effector functions, and a highly prolific SSClow population that can give rise to the replenishment of the memory pool. CAR-T cells derived from the younger SSClow population possess desirable attributes for immunotherapy, manifested by increased naïve-like cells and stem cell memory (TSCM )-like cells together with a balanced CD4/CD8 ratio, as well as enhanced target-killing in vitro and in vivo. Altogether, lymphocyte segregation based on biophysical properties is an effective approach for label-free selection of cells that share collective functions and can have important applications for cell-based immunotherapies | ||
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| 650 | 4 | |a immunotherapy | |
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| 650 | 4 | |a side scatter | |
| 700 | 1 | |a Tan, Joel Heng Loong |e verfasserin |4 aut | |
| 700 | 1 | |a Sin, Wei-Xiang |e verfasserin |4 aut | |
| 700 | 1 | |a Luah, Yen Hoon |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Sue Yee |e verfasserin |4 aut | |
| 700 | 1 | |a Goh, Myra |e verfasserin |4 aut | |
| 700 | 1 | |a Birnbaum, Michael E |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Qingfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Cheow, Lih Feng |e verfasserin |4 aut | |
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