Total RNA sequencing reveals gene expression and microbial alterations shared by oral pre-malignant lesions and cancer
© 2023. BioMed Central Ltd., part of Springer Nature..
Head and neck cancers are a complex malignancy comprising multiple anatomical sites, with cancer of the oral cavity ranking among the deadliest and the most disfiguring cancers globally. Oral cancer (OC) constitutes a subset of head and neck cancer cases, presenting primarily as tobacco- and alcohol-associated oral squamous cell carcinoma (OSCC), with a 5-year survival rate of ~ 65%, partly due to the lack of early detection and effective treatments. OSCC arises from premalignant lesions (PMLs) in the oral cavity through a multi-step series of clinical and histopathological stages, including varying degrees of epithelial dysplasia. To gain insights into the molecular mechanisms associated with the progression of PMLs to OSCC, we profiled the whole transcriptome of 66 human PMLs comprising leukoplakia with dysplasia and hyperkeratosis non-reactive (HkNR) pathologies, alongside healthy controls and OSCC. Our data revealed that PMLs were enriched in gene signatures associated with cellular plasticity, such as partial EMT (p-EMT) phenotypes, and with immune response. Integrated analyses of the host transcriptome and microbiome further highlighted a significant association between differential microbial abundance and PML pathway activity, suggesting a contribution of the oral microbiome toward PML evolution to OSCC. Collectively, this study reveals molecular processes associated with PML progression that may help early diagnosis and disease interception at an early stage.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
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| Enthalten in: |
Human genomics - 17(2023), 1 vom: 04. Aug., Seite 72 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Khan, Mohammed Muzamil [VerfasserIn] |
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| Links: |
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| Themen: |
Early detection |
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| Anmerkungen: |
Date Completed 07.08.2023 Date Revised 31.01.2024 published: Electronic UpdateOf: bioRxiv. 2023 Mar 24;:. - PMID 36993637 Citation Status MEDLINE |
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| doi: |
10.1186/s40246-023-00519-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. BioMed Central Ltd., part of Springer Nature. | ||
| 520 | |a Head and neck cancers are a complex malignancy comprising multiple anatomical sites, with cancer of the oral cavity ranking among the deadliest and the most disfiguring cancers globally. Oral cancer (OC) constitutes a subset of head and neck cancer cases, presenting primarily as tobacco- and alcohol-associated oral squamous cell carcinoma (OSCC), with a 5-year survival rate of ~ 65%, partly due to the lack of early detection and effective treatments. OSCC arises from premalignant lesions (PMLs) in the oral cavity through a multi-step series of clinical and histopathological stages, including varying degrees of epithelial dysplasia. To gain insights into the molecular mechanisms associated with the progression of PMLs to OSCC, we profiled the whole transcriptome of 66 human PMLs comprising leukoplakia with dysplasia and hyperkeratosis non-reactive (HkNR) pathologies, alongside healthy controls and OSCC. Our data revealed that PMLs were enriched in gene signatures associated with cellular plasticity, such as partial EMT (p-EMT) phenotypes, and with immune response. Integrated analyses of the host transcriptome and microbiome further highlighted a significant association between differential microbial abundance and PML pathway activity, suggesting a contribution of the oral microbiome toward PML evolution to OSCC. Collectively, this study reveals molecular processes associated with PML progression that may help early diagnosis and disease interception at an early stage | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Early detection | |
| 650 | 4 | |a Oral cancer | |
| 650 | 4 | |a Oral microbiota | |
| 650 | 4 | |a Pre-malignant lesions | |
| 700 | 1 | |a Frustino, Jennifer |e verfasserin |4 aut | |
| 700 | 1 | |a Villa, Alessandro |e verfasserin |4 aut | |
| 700 | 1 | |a Nguyen, Bach-Cuc |e verfasserin |4 aut | |
| 700 | 1 | |a Woo, Sook-Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Johnson, William Evan |e verfasserin |4 aut | |
| 700 | 1 | |a Varelas, Xaralabos |e verfasserin |4 aut | |
| 700 | 1 | |a Kukuruzinska, Maria |e verfasserin |4 aut | |
| 700 | 1 | |a Monti, Stefano |e verfasserin |4 aut | |
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