Structure determination of a DNA crystal by MicroED
Copyright © 2023 Elsevier Ltd. All rights reserved..
Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG)2 DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG)2 DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
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| Enthalten in: |
Structure (London, England : 1993) - 31(2023), 12 vom: 07. Dez., Seite 1499-1503.e2 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Haymaker, Alison [VerfasserIn] |
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| Links: |
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| Themen: |
Cryo-electron microscopy |
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| Anmerkungen: |
Date Completed 16.12.2023 Date Revised 18.02.2024 published: Print-Electronic UpdateOf: bioRxiv. 2023 Apr 26;:. - PMID 37163108 Citation Status MEDLINE |
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| doi: |
10.1016/j.str.2023.07.005 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM360409385 |
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| 520 | |a Copyright © 2023 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG)2 DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG)2 DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a DNA | |
| 650 | 4 | |a cryo-electron microscopy | |
| 650 | 4 | |a cryo-focused ion beam milling | |
| 650 | 4 | |a microcrystal electron diffraction, MicroED | |
| 650 | 4 | |a nucleic acids | |
| 700 | 1 | |a Bardin, Andrey A |e verfasserin |4 aut | |
| 700 | 1 | |a Gonen, Tamir |e verfasserin |4 aut | |
| 700 | 1 | |a Martynowycz, Michael W |e verfasserin |4 aut | |
| 700 | 1 | |a Nannenga, Brent L |e verfasserin |4 aut | |
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