TET2-mediated mRNA demethylation regulates leukemia stem cell homing and self-renewal
Copyright © 2023 Elsevier Inc. All rights reserved..
TET2 is recurrently mutated in acute myeloid leukemia (AML) and its deficiency promotes leukemogenesis (driven by aggressive oncogenic mutations) and enhances leukemia stem cell (LSC) self-renewal. However, the underlying cellular/molecular mechanisms have yet to be fully understood. Here, we show that Tet2 deficiency significantly facilitates leukemogenesis in various AML models (mediated by aggressive or less aggressive mutations) through promoting homing of LSCs into bone marrow (BM) niche to increase their self-renewal/proliferation. TET2 deficiency in AML blast cells increases expression of Tetraspanin 13 (TSPAN13) and thereby activates the CXCR4/CXCL12 signaling, leading to increased homing/migration of LSCs into BM niche. Mechanistically, TET2 deficiency results in the accumulation of methyl-5-cytosine (m5C) modification in TSPAN13 mRNA; YBX1 specifically recognizes the m5C modification and increases the stability and expression of TSPAN13 transcripts. Collectively, our studies reveal the functional importance of TET2 in leukemogenesis, leukemic blast cell migration/homing, and LSC self-renewal as an mRNA m5C demethylase.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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| Enthalten in: |
Cell stem cell - 30(2023), 8 vom: 03. Aug., Seite 1072-1090.e10 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Yangchan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.08.2023 Date Revised 07.08.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1016/j.stem.2023.07.001 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a TET2-mediated mRNA demethylation regulates leukemia stem cell homing and self-renewal |
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| 500 | |a Date Revised 07.08.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier Inc. All rights reserved. | ||
| 520 | |a TET2 is recurrently mutated in acute myeloid leukemia (AML) and its deficiency promotes leukemogenesis (driven by aggressive oncogenic mutations) and enhances leukemia stem cell (LSC) self-renewal. However, the underlying cellular/molecular mechanisms have yet to be fully understood. Here, we show that Tet2 deficiency significantly facilitates leukemogenesis in various AML models (mediated by aggressive or less aggressive mutations) through promoting homing of LSCs into bone marrow (BM) niche to increase their self-renewal/proliferation. TET2 deficiency in AML blast cells increases expression of Tetraspanin 13 (TSPAN13) and thereby activates the CXCR4/CXCL12 signaling, leading to increased homing/migration of LSCs into BM niche. Mechanistically, TET2 deficiency results in the accumulation of methyl-5-cytosine (m5C) modification in TSPAN13 mRNA; YBX1 specifically recognizes the m5C modification and increases the stability and expression of TSPAN13 transcripts. Collectively, our studies reveal the functional importance of TET2 in leukemogenesis, leukemic blast cell migration/homing, and LSC self-renewal as an mRNA m5C demethylase | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
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| 650 | 4 | |a RNA 5-methylcytosine methylation | |
| 650 | 4 | |a TET2 | |
| 650 | 4 | |a TSPAN13 | |
| 650 | 4 | |a YBX1 | |
| 650 | 4 | |a bone marrow microenvironment | |
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| 700 | 1 | |a Xue, Meilin |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Xiaolan |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Nguyen, Le Xuan Truong |e verfasserin |4 aut | |
| 700 | 1 | |a Ren, Lili |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Chenying |e verfasserin |4 aut | |
| 700 | 1 | |a Xue, Jianhuang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Zhicong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Qing, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Chao |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Brandon |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Zhenhua |e verfasserin |4 aut | |
| 700 | 1 | |a Leung, Keith |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Kitty |e verfasserin |4 aut | |
| 700 | 1 | |a Swaminathan, Srividya |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Wunderlich, Mark |e verfasserin |4 aut | |
| 700 | 1 | |a Mulloy, James C |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xiaobo |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Horne, David |e verfasserin |4 aut | |
| 700 | 1 | |a Rosen, Steven T |e verfasserin |4 aut | |
| 700 | 1 | |a Marcucci, Guido |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Mingjiang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Zejuan |e verfasserin |4 aut | |
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| 700 | 1 | |a Su, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Jianjun |e verfasserin |4 aut | |
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