Immunohistochemical, Flow Cytometric, and ELISA-Based Analyses of Intracellular, Membrane-Expressed, and Extracellular Hsp70 as Cancer Biomarkers
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
The major stress-inducible 70 kDa heat shock (stress) protein 70 (Hsp70) is frequently overexpressed in highly aggressive tumor cells and thus might serve as a tumor-specific biomarker of aggressive disease and/or therapeutic resistance. We have previously shown that, in contrast to normal cells, tumor cells present Hsp70 on their plasma membrane. In order to elucidate the role of intracellular, membrane-bound and extracellular Hsp70 as a potential tumor biomarker in cancer, herein we describe protocols for the staining of cytosolic Hsp70 in tumor formalin-fixed paraffin-embedded (FFPE) sections from patients with glioblastoma multiforme using immunohistochemistry, for detecting the expression of plasma membrane-bound Hsp70 by a range of cancer-derived cells using multi-parametric flow cytometry using the cmHsp70.1 monoclonal antibody (mAb) and for the measurement of free and vesicular-associated Hsp70 in the circulation of patients with cancer using a unique enzyme-linked immunosorbent assay (ELISA).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2693 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2693(2023) vom: 04., Seite 307-324 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dezfouli, Ali Bashiri [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.08.2023 Date Revised 07.08.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-3342-7_23 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM360401392 |
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| 245 | 1 | 0 | |a Immunohistochemical, Flow Cytometric, and ELISA-Based Analyses of Intracellular, Membrane-Expressed, and Extracellular Hsp70 as Cancer Biomarkers |
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| 500 | |a Date Revised 07.08.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. | ||
| 520 | |a The major stress-inducible 70 kDa heat shock (stress) protein 70 (Hsp70) is frequently overexpressed in highly aggressive tumor cells and thus might serve as a tumor-specific biomarker of aggressive disease and/or therapeutic resistance. We have previously shown that, in contrast to normal cells, tumor cells present Hsp70 on their plasma membrane. In order to elucidate the role of intracellular, membrane-bound and extracellular Hsp70 as a potential tumor biomarker in cancer, herein we describe protocols for the staining of cytosolic Hsp70 in tumor formalin-fixed paraffin-embedded (FFPE) sections from patients with glioblastoma multiforme using immunohistochemistry, for detecting the expression of plasma membrane-bound Hsp70 by a range of cancer-derived cells using multi-parametric flow cytometry using the cmHsp70.1 monoclonal antibody (mAb) and for the measurement of free and vesicular-associated Hsp70 in the circulation of patients with cancer using a unique enzyme-linked immunosorbent assay (ELISA) | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Cancer | |
| 650 | 4 | |a Enzyme-linked immunosorbent assay (ELISA) | |
| 650 | 4 | |a Extracellular and membrane-bound heat shock protein 70 (Hsp70) | |
| 650 | 4 | |a Flow cytometry | |
| 650 | 4 | |a Formalin-fixed paraffin-embedded (FFPE) sections | |
| 650 | 4 | |a Glioblastoma multiforme | |
| 650 | 4 | |a Hsp70 antibody epitope | |
| 650 | 4 | |a Immunohistochemistry | |
| 650 | 4 | |a Intracellular | |
| 650 | 7 | |a Biomarkers, Tumor |2 NLM | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a HSP70 Heat-Shock Proteins |2 NLM | |
| 700 | 1 | |a Stangl, Stefan |e verfasserin |4 aut | |
| 700 | 1 | |a Foulds, Gemma A |e verfasserin |4 aut | |
| 700 | 1 | |a Lennartz, Philipp |e verfasserin |4 aut | |
| 700 | 1 | |a Pilkington, Geoffrey J |e verfasserin |4 aut | |
| 700 | 1 | |a Pockley, A Graham |e verfasserin |4 aut | |
| 700 | 1 | |a Multhoff, Gabriele |e verfasserin |4 aut | |
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