An efficient and recyclable electroeluter : from homemade to modular design for potential mass production
Electrophoresis is one of the most powerful techniques to separate nucleic acids or protein molecules. The recovery of purified components from the gel is key to downstream analysis or function study. Here, we provide a cost-effective electroeluter in both homemade and module-assembled versions. The recovery yield can reach as high as >90% for single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), and protein in practical testing, which outperforms a commercial kit as well as a purchased electroeluter. It fully addresses the existing concerns in this field. First of all, for almost all kits, there remains ambiguity in recovering ssDNA to satisfy specific demands, which is generally ignored. Secondly, the recovery of dsDNA from agarose gel with consumables is vulnerable to a lot of factors and involves chemicals/materials that are not friendly to the environment and operating personnel. Thirdly, recovery from polyacrylamide matrices is very difficult, and the most exploited diffusion method through crush-and-soak suffers from low yield even after a long-time diffusion. Lastly, there is a universal problem in scaling up, especially for commercial electroeluters. The present electroelution method addresses the above issues, and it is believed that it will facilitate associated research and find widespread application.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
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| Enthalten in: |
Lab on a chip - 23(2023), 17 vom: 22. Aug., Seite 3874-3881 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Su, Linhan [VerfasserIn] |
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| Links: |
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| Themen: |
9007-49-2 |
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| Anmerkungen: |
Date Completed 23.08.2023 Date Revised 30.08.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1039/d3lc00428g |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM360394167 |
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| 520 | |a Electrophoresis is one of the most powerful techniques to separate nucleic acids or protein molecules. The recovery of purified components from the gel is key to downstream analysis or function study. Here, we provide a cost-effective electroeluter in both homemade and module-assembled versions. The recovery yield can reach as high as >90% for single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), and protein in practical testing, which outperforms a commercial kit as well as a purchased electroeluter. It fully addresses the existing concerns in this field. First of all, for almost all kits, there remains ambiguity in recovering ssDNA to satisfy specific demands, which is generally ignored. Secondly, the recovery of dsDNA from agarose gel with consumables is vulnerable to a lot of factors and involves chemicals/materials that are not friendly to the environment and operating personnel. Thirdly, recovery from polyacrylamide matrices is very difficult, and the most exploited diffusion method through crush-and-soak suffers from low yield even after a long-time diffusion. Lastly, there is a universal problem in scaling up, especially for commercial electroeluters. The present electroelution method addresses the above issues, and it is believed that it will facilitate associated research and find widespread application | ||
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