Toward Single Cell Tattoos : Biotransfer Printing of Lithographic Gold Nanopatterns on Live Cells
Lithographic nanopatterning techniques such as photolithography, electron-beam lithography, and nanoimprint lithography (NIL) have revolutionized modern-day electronics and optics. Yet, their application for creating nanobio interfaces is limited by the cytotoxic and two-dimensional nature of conventional fabrication methods. Here, we present a biocompatible and cost-effective transfer process that leverages (a) NIL to define sub-300 nm gold (Au) nanopattern arrays, (b) amine functionalization of Au to transfer the NIL-arrays from a rigid substrate to a soft transfer layer, (c) alginate hydrogel as a flexible, degradable transfer layer, and (d) gelatin conjugation of the Au NIL-arrays to achieve conformal contact with live cells. We demonstrate biotransfer printing of the Au NIL-arrays on rat brains and live cells with high pattern fidelity and cell viability and observed differences in cell migration on the Au NIL-dot and NIL-wire printed hydrogels. We anticipate that this nanolithography-compatible biotransfer printing method could advance bionics, biosensing, and biohybrid tissue interfaces.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
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| Enthalten in: |
Nano letters - 23(2023), 16 vom: 23. Aug., Seite 7477-7484 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kwok, Kam Sang [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 24.08.2023 Date Revised 22.01.2024 published: Print-Electronic UpdateOf: bioRxiv. 2023 May 28;:. - PMID 37292601 Citation Status MEDLINE |
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| doi: |
10.1021/acs.nanolett.3c01960 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM360260136 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Lithographic nanopatterning techniques such as photolithography, electron-beam lithography, and nanoimprint lithography (NIL) have revolutionized modern-day electronics and optics. Yet, their application for creating nanobio interfaces is limited by the cytotoxic and two-dimensional nature of conventional fabrication methods. Here, we present a biocompatible and cost-effective transfer process that leverages (a) NIL to define sub-300 nm gold (Au) nanopattern arrays, (b) amine functionalization of Au to transfer the NIL-arrays from a rigid substrate to a soft transfer layer, (c) alginate hydrogel as a flexible, degradable transfer layer, and (d) gelatin conjugation of the Au NIL-arrays to achieve conformal contact with live cells. We demonstrate biotransfer printing of the Au NIL-arrays on rat brains and live cells with high pattern fidelity and cell viability and observed differences in cell migration on the Au NIL-dot and NIL-wire printed hydrogels. We anticipate that this nanolithography-compatible biotransfer printing method could advance bionics, biosensing, and biohybrid tissue interfaces | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a biosensors | |
| 650 | 4 | |a cell culture | |
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| 700 | 1 | |a Pahapale, Gayatri J |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Luo |e verfasserin |4 aut | |
| 700 | 1 | |a Gracias, David H |e verfasserin |4 aut | |
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