Exosomes from Adipose Tissue-derived Mesenchymal Stem Cells Induce Regulatory T Cells in COVID-19 Patients
An imbalance between regulatory T (Treg) and T-helper (Th)-17 cells has been implicated in the pathogenesis of coronavirus disease 2019 (COVID-19). Mesenchymal stem cells (MSCs) exert immunomodulatory properties through secreting exosomes. This study aimed to assess the effect of MSC-derived exosomes (MSC-Exo) on the differentiation of peripheral blood mononuclear cells (PBMCs) into Tregs from patients with COVID-19. Exosomes were isolated from adipose tissue-derived MSCs. PBMCs were separated from the whole blood of COVID-19 patients (n=20). Treg frequency was assessed before and 48 hours after treatment of PBMCs with MSC-Exo using flow cytometry. Expression of FOXP3 and cytokine genes, and the concentration of cytokines associated with Tregs, were assessed before and after treatment with MSC-Exo. The frequency of CD4+CD25+CD127- Tregs was significantly higher after treating PBMCs with MSC-Exo (6.695±2.528) compared to before treatment (4.981±2.068). The expressions of transforming growth factor (TGF)-β1, interleukin (IL)-10, and FOXP3 were significantly upregulated in MSC-Exo-treated PBMCs. The concentration of IL-10 increased significantly after treatment (994.7±543.9 pg/mL) of PBMCs with MSC-Exo compared with before treatment (563.5±408.6 pg/mL). The concentration of TGF-β was significantly higher in the supernatant of PBMCs after treatment with MSC-Exo (477.0±391.1 pg/mL) than PBMCs before treatment (257.7±226.3 pg/mL). MSC-Exo has the potential to raise anti-inflammatory responses by induction of Tregs, potentiating its therapeutic effects in COVID-19.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
Iranian journal of allergy, asthma, and immunology - 22(2023), 3 vom: 16. Juni, Seite 233-244 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Motallebnezhad, Morteza [VerfasserIn] |
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| Links: |
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| Themen: |
COVID-19 |
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| Anmerkungen: |
Date Completed 23.10.2023 Date Revised 23.10.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.18502/ijaai.v22i3.13051 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM360244858 |
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| 520 | |a An imbalance between regulatory T (Treg) and T-helper (Th)-17 cells has been implicated in the pathogenesis of coronavirus disease 2019 (COVID-19). Mesenchymal stem cells (MSCs) exert immunomodulatory properties through secreting exosomes. This study aimed to assess the effect of MSC-derived exosomes (MSC-Exo) on the differentiation of peripheral blood mononuclear cells (PBMCs) into Tregs from patients with COVID-19. Exosomes were isolated from adipose tissue-derived MSCs. PBMCs were separated from the whole blood of COVID-19 patients (n=20). Treg frequency was assessed before and 48 hours after treatment of PBMCs with MSC-Exo using flow cytometry. Expression of FOXP3 and cytokine genes, and the concentration of cytokines associated with Tregs, were assessed before and after treatment with MSC-Exo. The frequency of CD4+CD25+CD127- Tregs was significantly higher after treating PBMCs with MSC-Exo (6.695±2.528) compared to before treatment (4.981±2.068). The expressions of transforming growth factor (TGF)-β1, interleukin (IL)-10, and FOXP3 were significantly upregulated in MSC-Exo-treated PBMCs. The concentration of IL-10 increased significantly after treatment (994.7±543.9 pg/mL) of PBMCs with MSC-Exo compared with before treatment (563.5±408.6 pg/mL). The concentration of TGF-β was significantly higher in the supernatant of PBMCs after treatment with MSC-Exo (477.0±391.1 pg/mL) than PBMCs before treatment (257.7±226.3 pg/mL). MSC-Exo has the potential to raise anti-inflammatory responses by induction of Tregs, potentiating its therapeutic effects in COVID-19 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a Exosomes | |
| 650 | 4 | |a Immunomodulation | |
| 650 | 4 | |a Mesenchymal stem cells | |
| 650 | 4 | |a Peripheral blood mononuclear cells | |
| 650 | 4 | |a Regulatory T cells | |
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| 700 | 1 | |a Hazrati, Ali |e verfasserin |4 aut | |
| 700 | 1 | |a Esmaeili Gouvarchin Ghaleh, Hadi |e verfasserin |4 aut | |
| 700 | 1 | |a Jonaidi-Jafari, Nematollah |e verfasserin |4 aut | |
| 700 | 1 | |a Abbaspour-Aghdam, Sanaz |e verfasserin |4 aut | |
| 700 | 1 | |a Malekpour, Kosar |e verfasserin |4 aut | |
| 700 | 1 | |a Yousefi, Mehdi |e verfasserin |4 aut | |
| 700 | 1 | |a Samadi Kafil, Hossein |e verfasserin |4 aut | |
| 700 | 1 | |a Jadidi-Niaragh, Farhad |e verfasserin |4 aut | |
| 700 | 1 | |a Roshangar, Leila |e verfasserin |4 aut | |
| 700 | 1 | |a Valizadeh, Hamed |e verfasserin |4 aut | |
| 700 | 1 | |a Izadi, Morteza |e verfasserin |4 aut | |
| 700 | 1 | |a Ahmadi, Majid |e verfasserin |4 aut | |
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