SARS-CoV-2 Prevalence and Variant Surveillance among Cats in Pittsburgh, Pennsylvania, USA
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infects many mammals, and SARS-CoV-2 circulation in nonhuman animals may increase the risk of novel variant emergence. Cats are highly susceptible to SARS-CoV-2 infection, and there were cases of virus transmission between cats and humans. The objective of this study was to assess the prevalence of SARS-CoV-2 variant infection of cats in an urban setting. We investigated the prevalence of SARS-CoV-2 variant infections in domestic and community cats in the city of Pittsburgh (n = 272). While no cats tested positive for SARS-CoV-2 viral RNA, 35 cats (12.86%) tested SARS-CoV-2-antibody-positive. Further, we compared a cat-specific experimental lateral flow assay (eLFA) and species-agnostic surrogate virus neutralization assay (sVNT) for SARS-CoV-2 antibody detection in cats (n = 71). The eLFA demonstrated 100% specificity compared to sVNT. The eLFA also showed 100% sensitivity for sera with >90% inhibition and 63.63% sensitivity for sera with 40-89% inhibition in sVNT. Using a variant-specific pseudovirus neutralization assay (pVNT) and antigen cartography, we found the presence of antibodies to pre-Omicron and Omicron SARS-CoV-2 variants. Hence, this approach proves valuable in identifying cat exposure to different SARS-CoV-2 variants. Our results highlight the continued exposure of cats to SARS-CoV-2 and warrant coordinated surveillance efforts.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Viruses - 15(2023), 7 vom: 30. Juni |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ramasamy, Santhamani [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 31.07.2023 Date Revised 03.08.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/v15071493 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM360150500 |
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| 100 | 1 | |a Ramasamy, Santhamani |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a SARS-CoV-2 Prevalence and Variant Surveillance among Cats in Pittsburgh, Pennsylvania, USA |
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| 500 | |a Date Completed 31.07.2023 | ||
| 500 | |a Date Revised 03.08.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infects many mammals, and SARS-CoV-2 circulation in nonhuman animals may increase the risk of novel variant emergence. Cats are highly susceptible to SARS-CoV-2 infection, and there were cases of virus transmission between cats and humans. The objective of this study was to assess the prevalence of SARS-CoV-2 variant infection of cats in an urban setting. We investigated the prevalence of SARS-CoV-2 variant infections in domestic and community cats in the city of Pittsburgh (n = 272). While no cats tested positive for SARS-CoV-2 viral RNA, 35 cats (12.86%) tested SARS-CoV-2-antibody-positive. Further, we compared a cat-specific experimental lateral flow assay (eLFA) and species-agnostic surrogate virus neutralization assay (sVNT) for SARS-CoV-2 antibody detection in cats (n = 71). The eLFA demonstrated 100% specificity compared to sVNT. The eLFA also showed 100% sensitivity for sera with >90% inhibition and 63.63% sensitivity for sera with 40-89% inhibition in sVNT. Using a variant-specific pseudovirus neutralization assay (pVNT) and antigen cartography, we found the presence of antibodies to pre-Omicron and Omicron SARS-CoV-2 variants. Hence, this approach proves valuable in identifying cat exposure to different SARS-CoV-2 variants. Our results highlight the continued exposure of cats to SARS-CoV-2 and warrant coordinated surveillance efforts | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
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| 700 | 1 | |a Neerukonda, Sabarinath |e verfasserin |4 aut | |
| 700 | 1 | |a Ruggiero, Diana |e verfasserin |4 aut | |
| 700 | 1 | |a Morrow, Becky |e verfasserin |4 aut | |
| 700 | 1 | |a Gupta, Sheweta |e verfasserin |4 aut | |
| 700 | 1 | |a Amirthalingam, Saranya |e verfasserin |4 aut | |
| 700 | 1 | |a Hardham, John M |e verfasserin |4 aut | |
| 700 | 1 | |a Lizer, Joshua T |e verfasserin |4 aut | |
| 700 | 1 | |a Yon, Michele |e verfasserin |4 aut | |
| 700 | 1 | |a Nissly, Ruth H |e verfasserin |4 aut | |
| 700 | 1 | |a Jakka, Padmaja |e verfasserin |4 aut | |
| 700 | 1 | |a Chothe, Shubhada K |e verfasserin |4 aut | |
| 700 | 1 | |a LaBella, Lindsey C |e verfasserin |4 aut | |
| 700 | 1 | |a Tewari, Deepanker |e verfasserin |4 aut | |
| 700 | 1 | |a Nair, Meera Surendran |e verfasserin |4 aut | |
| 700 | 1 | |a Kuchipudi, Suresh V |e verfasserin |4 aut | |
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