Altered expression of apoptosis-related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation
© 2023 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd..
AIM: Impaired apoptosis and proliferation resulted in autoreactive lymphocyte development and inflammation in Rheumatoid arthritis (RA). TP53, BAX, FOXO1, and RB1 are related genes in cell survival, proliferation, and inflammation which could be important in RA development and disease severity. Here we investigated their expression in peripheral blood mononuclear cells (PBMCs) from RA patients in comparison to healthy controls.
METHODS: Fifty healthy controls and 50 RA patients were selected. The quantitative real-time polymerase chain reaction was used to assess the gene expression level in PBMCs.
RESULTS: The mRNA expression of TP53 (FC = 0.65, p = .000), BAX (FC = 0.76, p = .008), FOXO1 (FC = 0.59, p = .000) and RB1 (FC = 0.50, p = .000) were significantly reduced in RA PBMCs. TP53 expression was negatively correlated with miR-16-5p (p = .032) and FOXO1 expression was negatively correlated with miR-335-5p (p = .005) and miR-34a-5p (p = .014). A positive correlation was seen between TP53 expression and its downstream gene, BAX (p = .001). FOXO1 expression was also negatively correlated with disease activity, DAS28 (p = .021).
CONCLUSION: All selected genes have downregulated expression in RA PBMCs which could be correlated with RA pathogenesis by regulating apoptosis, cell survival, inflammatory mediator production, and proliferation. Due to the correlation of miR-16-5p, miR-34a-5p, and miR-335-5p with TP53 and FOXO1 expression in RA PBMCs, they could be used as future therapeutic targets.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Immunity, inflammation and disease - 11(2023), 7 vom: 01. Juli, Seite e914 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ebrahimian, Hamidreza [VerfasserIn] |
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| Links: |
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| Themen: |
Apoptosis |
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| Anmerkungen: |
Date Completed 31.07.2023 Date Revised 31.07.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1002/iid3.914 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM360060382 |
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| 100 | 1 | |a Ebrahimian, Hamidreza |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Altered expression of apoptosis-related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Revised 31.07.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. | ||
| 520 | |a AIM: Impaired apoptosis and proliferation resulted in autoreactive lymphocyte development and inflammation in Rheumatoid arthritis (RA). TP53, BAX, FOXO1, and RB1 are related genes in cell survival, proliferation, and inflammation which could be important in RA development and disease severity. Here we investigated their expression in peripheral blood mononuclear cells (PBMCs) from RA patients in comparison to healthy controls | ||
| 520 | |a METHODS: Fifty healthy controls and 50 RA patients were selected. The quantitative real-time polymerase chain reaction was used to assess the gene expression level in PBMCs | ||
| 520 | |a RESULTS: The mRNA expression of TP53 (FC = 0.65, p = .000), BAX (FC = 0.76, p = .008), FOXO1 (FC = 0.59, p = .000) and RB1 (FC = 0.50, p = .000) were significantly reduced in RA PBMCs. TP53 expression was negatively correlated with miR-16-5p (p = .032) and FOXO1 expression was negatively correlated with miR-335-5p (p = .005) and miR-34a-5p (p = .014). A positive correlation was seen between TP53 expression and its downstream gene, BAX (p = .001). FOXO1 expression was also negatively correlated with disease activity, DAS28 (p = .021) | ||
| 520 | |a CONCLUSION: All selected genes have downregulated expression in RA PBMCs which could be correlated with RA pathogenesis by regulating apoptosis, cell survival, inflammatory mediator production, and proliferation. Due to the correlation of miR-16-5p, miR-34a-5p, and miR-335-5p with TP53 and FOXO1 expression in RA PBMCs, they could be used as future therapeutic targets | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a BAX | |
| 650 | 4 | |a FOXO1 | |
| 650 | 4 | |a P53 | |
| 650 | 4 | |a RB1 | |
| 650 | 4 | |a apoptosis | |
| 650 | 4 | |a autoimmunity | |
| 650 | 4 | |a miRNAs | |
| 650 | 4 | |a rheumatoid arthritis (RA) | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a bcl-2-Associated X Protein |2 NLM | |
| 700 | 1 | |a Akhtari, Maryam |e verfasserin |4 aut | |
| 700 | 1 | |a Akhlaghi, Maassoumeh |e verfasserin |4 aut | |
| 700 | 1 | |a Farhadi, Elham |e verfasserin |4 aut | |
| 700 | 1 | |a Jamshidi, Ahmadreza |e verfasserin |4 aut | |
| 700 | 1 | |a Alishiri, Gholam Hossein |e verfasserin |4 aut | |
| 700 | 1 | |a Mahmoudi, Mahdi |e verfasserin |4 aut | |
| 700 | 1 | |a Tavallaie, Mahmood |e verfasserin |4 aut | |
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