Genetic modification of inflammation- and clonal hematopoiesis-associated cardiovascular risk
Clonal hematopoiesis of indeterminate potential (CHIP) is associated with an increased risk of cardiovascular diseases (CVDs), putatively via inflammasome activation. We pursued an inflammatory gene modifier scan for CHIP-associated CVD risk among 424,651 UK Biobank participants. We identified CHIP using whole-exome sequencing data of blood DNA and modeled as a composite, considering all driver genes together, as well as separately for common drivers (DNMT3A, TET2, ASXL1, and JAK2). We developed predicted gene expression scores for 26 inflammasome-related genes and assessed how they modify CHIP-associated CVD risk. We identified IL1RAP as a potential key molecule for CHIP-associated CVD risk across genes and increased AIM2 gene expression leading to heightened JAK2- and ASXL1-associated CVD risk. We show that CRISPR-induced Asxl1-mutated murine macrophages had a particularly heightened inflammatory response to AIM2 agonism, associated with an increased DNA damage response, as well as increased IL-10 secretion, mirroring a CVD-protective effect of IL10 expression in ASXL1 CHIP. Our study supports the role of inflammasomes in CHIP-associated CVD and provides evidence to support gene-specific strategies to address CHIP-associated CVD risk.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:133 |
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| Enthalten in: |
The Journal of clinical investigation - 133(2023), 18 vom: 15. Sept. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yu, Zhi [VerfasserIn] |
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| Links: |
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| Themen: |
Cardiology |
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| Anmerkungen: |
Date Completed 18.09.2023 Date Revised 02.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1172/JCI168597 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Genetic modification of inflammation- and clonal hematopoiesis-associated cardiovascular risk |
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| 500 | |a Date Revised 02.03.2024 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Clonal hematopoiesis of indeterminate potential (CHIP) is associated with an increased risk of cardiovascular diseases (CVDs), putatively via inflammasome activation. We pursued an inflammatory gene modifier scan for CHIP-associated CVD risk among 424,651 UK Biobank participants. We identified CHIP using whole-exome sequencing data of blood DNA and modeled as a composite, considering all driver genes together, as well as separately for common drivers (DNMT3A, TET2, ASXL1, and JAK2). We developed predicted gene expression scores for 26 inflammasome-related genes and assessed how they modify CHIP-associated CVD risk. We identified IL1RAP as a potential key molecule for CHIP-associated CVD risk across genes and increased AIM2 gene expression leading to heightened JAK2- and ASXL1-associated CVD risk. We show that CRISPR-induced Asxl1-mutated murine macrophages had a particularly heightened inflammatory response to AIM2 agonism, associated with an increased DNA damage response, as well as increased IL-10 secretion, mirroring a CVD-protective effect of IL10 expression in ASXL1 CHIP. Our study supports the role of inflammasomes in CHIP-associated CVD and provides evidence to support gene-specific strategies to address CHIP-associated CVD risk | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Cardiology | |
| 650 | 4 | |a Cardiovascular disease | |
| 650 | 4 | |a Genetics | |
| 650 | 4 | |a Mouse models | |
| 650 | 4 | |a Population genetics | |
| 650 | 7 | |a Inflammasomes |2 NLM | |
| 700 | 1 | |a Fidler, Trevor P |e verfasserin |4 aut | |
| 700 | 1 | |a Ruan, Yunfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Vlasschaert, Caitlyn |e verfasserin |4 aut | |
| 700 | 1 | |a Nakao, Tetsushi |e verfasserin |4 aut | |
| 700 | 1 | |a Uddin, Md Mesbah |e verfasserin |4 aut | |
| 700 | 1 | |a Mack, Taralynn |e verfasserin |4 aut | |
| 700 | 1 | |a Niroula, Abhishek |e verfasserin |4 aut | |
| 700 | 1 | |a Heimlich, J Brett |e verfasserin |4 aut | |
| 700 | 1 | |a Zekavat, Seyedeh M |e verfasserin |4 aut | |
| 700 | 1 | |a Gibson, Christopher J |e verfasserin |4 aut | |
| 700 | 1 | |a Griffin, Gabriel K |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yuxuan |e verfasserin |4 aut | |
| 700 | 1 | |a Peloso, Gina M |e verfasserin |4 aut | |
| 700 | 1 | |a Heard-Costa, Nancy |e verfasserin |4 aut | |
| 700 | 1 | |a Levy, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Vasan, Ramachandran S |e verfasserin |4 aut | |
| 700 | 1 | |a Aguet, François |e verfasserin |4 aut | |
| 700 | 1 | |a Ardlie, Kristin G |e verfasserin |4 aut | |
| 700 | 1 | |a Taylor, Kent D |e verfasserin |4 aut | |
| 700 | 1 | |a Rich, Stephen S |e verfasserin |4 aut | |
| 700 | 1 | |a Rotter, Jerome I |e verfasserin |4 aut | |
| 700 | 1 | |a Libby, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Jaiswal, Siddhartha |e verfasserin |4 aut | |
| 700 | 1 | |a Ebert, Benjamin L |e verfasserin |4 aut | |
| 700 | 1 | |a Bick, Alexander G |e verfasserin |4 aut | |
| 700 | 1 | |a Tall, Alan R |e verfasserin |4 aut | |
| 700 | 1 | |a Natarajan, Pradeep |e verfasserin |4 aut | |
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