Nanomanipulation of Ligand Nanogeometry Modulates Integrin/Clathrin-Mediated Adhesion and Endocytosis of Stem Cells
Nanosubstrate engineering can be a biomechanical approach for modulating stem cell differentiation in tissue engineering. However, the study of the effect of clathrin-mediated processes on manipulating this behavior is unexplored. Herein, we develop integrin-binding nanosubstrates with confined nanogeometries that regulate clathrin-mediated adhesion- or endocytosis-active signaling pathways for modulating stem fates. Isotropically presenting ligands on the nanoscale enhances the expression of clathrin in cells, thereby facilitating uptake of dexamethasone-loaded nanoparticles (NPs) to boost osteogenesis of stem cells. In contrast, anisotropic ligand nanogeometry suppresses this clathrin-mediated NP entry by strengthening the association between clathrin and adhesion spots to reinforce mechanotransduced signaling, which can be abrogated by the pharmacological inhibition of clathrin. Meanwhile, inhibiting focal adhesion formation hinders cell spreading and enables a higher endocytosis efficiency. Our findings reveal the crucial roles of clathrin in both endocytosis and mechanotransduction of stem cells and provide the parameter of ligand nanogeometry for the rational design of biomaterials for tissue engineering.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
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| Enthalten in: |
Nano letters - 23(2023), 19 vom: 11. Okt., Seite 9160-9169 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yin, Bohan [VerfasserIn] |
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| Links: |
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| Themen: |
Cell−substrate interaction |
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| Anmerkungen: |
Date Completed 12.10.2023 Date Revised 16.10.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.nanolett.3c01757 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM359943381 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Nanosubstrate engineering can be a biomechanical approach for modulating stem cell differentiation in tissue engineering. However, the study of the effect of clathrin-mediated processes on manipulating this behavior is unexplored. Herein, we develop integrin-binding nanosubstrates with confined nanogeometries that regulate clathrin-mediated adhesion- or endocytosis-active signaling pathways for modulating stem fates. Isotropically presenting ligands on the nanoscale enhances the expression of clathrin in cells, thereby facilitating uptake of dexamethasone-loaded nanoparticles (NPs) to boost osteogenesis of stem cells. In contrast, anisotropic ligand nanogeometry suppresses this clathrin-mediated NP entry by strengthening the association between clathrin and adhesion spots to reinforce mechanotransduced signaling, which can be abrogated by the pharmacological inhibition of clathrin. Meanwhile, inhibiting focal adhesion formation hinders cell spreading and enables a higher endocytosis efficiency. Our findings reveal the crucial roles of clathrin in both endocytosis and mechanotransduction of stem cells and provide the parameter of ligand nanogeometry for the rational design of biomaterials for tissue engineering | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Nanosubstrate engineering | |
| 650 | 4 | |a cell−substrate interaction | |
| 650 | 4 | |a endocytosis | |
| 650 | 4 | |a ligand nanogeometry | |
| 650 | 4 | |a stem cell differentiation | |
| 650 | 7 | |a Integrins |2 NLM | |
| 650 | 7 | |a Clathrin |2 NLM | |
| 650 | 7 | |a Ligands |2 NLM | |
| 700 | 1 | |a Zhang, Qin |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Jiaxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Yingying |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Chuanqi |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Jiareng |e verfasserin |4 aut | |
| 700 | 1 | |a Wen, Chunyi |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Siu Hong Dexter |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Mo |e verfasserin |4 aut | |
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