Preparation of silk fibroin/hyaluronic acid composite hydrogel based on thiol-ene click chemistry
OBJECTIVES: To design and prepare silk fibroin/hyaluronic acid composite hydrogel.
METHODS: The thiol modified silk fibroin and the double-bond modified hyaluronic acid were rapidly cured into gels through thiol-ene click polymerization under ultraviolet light condition. The grafting rate of modified silk fibroin and hyaluronic acid was characterized by 1H NMR spectroscopy; the gel point and the internal microstructure of hydrogels were characterized by rheological test and scanning electron microscopy; the mechanical properties were characterized by compression test; the swelling rate and degradation rate were determined by mass method. The hydrogel was co-cultured with the cells, the cytotoxicity was measured by the lactate dehydrogenase method, the cell adhesion was measured by the float count method, and the cell growth and differentiation on the surface of the gel were observed by scanning electron microscope and fluorescence microscope.
RESULTS: The functional group substitution degrees of modified silk fibroin and hyaluronic acid were 17.99% and 48.03%, respectively. The prepared silk fibroin/hyaluronic acid composite hydrogel had a gel point of 40-60 s and had a porous structure inside the gel. The compressive strength was as high as 450 kPa and it would not break after ten cycles. The water absorption capacity of the composite hydrogel was 4-10 times of its own weight. Degradation experiments showed that the hydrogel was biodegradable, and the degradation rate reached 28%-42% after 35 d. The cell biology experiments showed that the cytotoxicity of the composite gel was low, the cell adhesion was good, and the growth and differentiation of the cells on the surface of the gel were good.
CONCLUSIONS: The photocurable silk fibroin/hyaluronic acid composite hydrogel can form a gel quickly, and has excellent mechanical properties, adjustable swelling rate and degradation degree, good biocompatibility, so it has promising application prospects in biomedicine.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:52 |
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| Enthalten in: |
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences - 52(2023), 3 vom: 25. Juni, Seite 285-295 |
| Sprache: |
Englisch |
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| Weiterer Titel: |
基于巯基-烯点击化学制备丝素蛋白/透明质酸复合水凝胶 |
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| Beteiligte Personen: |
Chen, Leidan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 24.07.2023 Date Revised 28.11.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.3724/zdxbyxb-2022-0702 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM359773516 |
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| 041 | |a eng | ||
| 100 | 1 | |a Chen, Leidan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Preparation of silk fibroin/hyaluronic acid composite hydrogel based on thiol-ene click chemistry |
| 246 | 3 | 3 | |a 基于巯基-烯点击化学制备丝素蛋白/透明质酸复合水凝胶 |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Completed 24.07.2023 | ||
| 500 | |a Date Revised 28.11.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVES: To design and prepare silk fibroin/hyaluronic acid composite hydrogel | ||
| 520 | |a METHODS: The thiol modified silk fibroin and the double-bond modified hyaluronic acid were rapidly cured into gels through thiol-ene click polymerization under ultraviolet light condition. The grafting rate of modified silk fibroin and hyaluronic acid was characterized by 1H NMR spectroscopy; the gel point and the internal microstructure of hydrogels were characterized by rheological test and scanning electron microscopy; the mechanical properties were characterized by compression test; the swelling rate and degradation rate were determined by mass method. The hydrogel was co-cultured with the cells, the cytotoxicity was measured by the lactate dehydrogenase method, the cell adhesion was measured by the float count method, and the cell growth and differentiation on the surface of the gel were observed by scanning electron microscope and fluorescence microscope | ||
| 520 | |a RESULTS: The functional group substitution degrees of modified silk fibroin and hyaluronic acid were 17.99% and 48.03%, respectively. The prepared silk fibroin/hyaluronic acid composite hydrogel had a gel point of 40-60 s and had a porous structure inside the gel. The compressive strength was as high as 450 kPa and it would not break after ten cycles. The water absorption capacity of the composite hydrogel was 4-10 times of its own weight. Degradation experiments showed that the hydrogel was biodegradable, and the degradation rate reached 28%-42% after 35 d. The cell biology experiments showed that the cytotoxicity of the composite gel was low, the cell adhesion was good, and the growth and differentiation of the cells on the surface of the gel were good | ||
| 520 | |a CONCLUSIONS: The photocurable silk fibroin/hyaluronic acid composite hydrogel can form a gel quickly, and has excellent mechanical properties, adjustable swelling rate and degradation degree, good biocompatibility, so it has promising application prospects in biomedicine | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Biocompatibility | |
| 650 | 4 | |a Hyaluronic acid | |
| 650 | 4 | |a Hydrogel | |
| 650 | 4 | |a Photo-initiated crosslinking | |
| 650 | 4 | |a Silk fibroin | |
| 650 | 4 | |a Thiol-ene click chemistry | |
| 650 | 7 | |a Fibroins |2 NLM | |
| 650 | 7 | |a 9007-76-5 |2 NLM | |
| 650 | 7 | |a Hydrogels |2 NLM | |
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| 650 | 7 | |a Biocompatible Materials |2 NLM | |
| 650 | 7 | |a Sulfhydryl Compounds |2 NLM | |
| 650 | 7 | |a Silk |2 NLM | |
| 700 | 1 | |a Zhong, Mingqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Jinyi |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Zhenjie |e verfasserin |4 aut | |
| 700 | 1 | |a Kuang, Tairong |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Tong |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Feng |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences |d 2002 |g 52(2023), 3 vom: 25. Juni, Seite 285-295 |w (DE-627)NLM123207037 |x 1008-9292 |7 nnns |
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