Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q
© 2023. The Author(s)..
Tuberculosis (TB) is a prevalent disease causing an estimated 1.6 million deaths and 10.6 million new cases annually. Discriminating TB disease from differential diagnoses can be complex, particularly in the field. Increased levels of complement component C1q in serum have been identified as a specific and accessible biomarker for TB disease but the source of C1q in circulation has not been identified. Here, data and samples previously collected from human cohorts, a clinical trial and a non-human primate study were used to identify cells producing C1q in circulation. Cell subset frequencies were correlated with serum C1q levels and combined with single cell RNA sequencing and flow cytometry analyses. This identified monocytes as C1q producers in circulation, with a pronounced expression of C1q in classical and intermediate monocytes and variable expression in non-classical monocytes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Scientific reports - 13(2023), 1 vom: 18. Juli, Seite 11617 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Niewold, Paula [VerfasserIn] |
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| Links: |
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| Themen: |
80295-33-6 |
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| Anmerkungen: |
Date Completed 21.07.2023 Date Revised 21.07.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-023-38889-x |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM359644821 |
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| 245 | 1 | 0 | |a Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q |
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| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Tuberculosis (TB) is a prevalent disease causing an estimated 1.6 million deaths and 10.6 million new cases annually. Discriminating TB disease from differential diagnoses can be complex, particularly in the field. Increased levels of complement component C1q in serum have been identified as a specific and accessible biomarker for TB disease but the source of C1q in circulation has not been identified. Here, data and samples previously collected from human cohorts, a clinical trial and a non-human primate study were used to identify cells producing C1q in circulation. Cell subset frequencies were correlated with serum C1q levels and combined with single cell RNA sequencing and flow cytometry analyses. This identified monocytes as C1q producers in circulation, with a pronounced expression of C1q in classical and intermediate monocytes and variable expression in non-classical monocytes | ||
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| 700 | 1 | |a Dijkstra, Douwe J |e verfasserin |4 aut | |
| 700 | 1 | |a Cai, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Goletti, Delia |e verfasserin |4 aut | |
| 700 | 1 | |a Palmieri, Fabrizio |e verfasserin |4 aut | |
| 700 | 1 | |a van Meijgaarden, Krista E |e verfasserin |4 aut | |
| 700 | 1 | |a Verreck, Frank A W |e verfasserin |4 aut | |
| 700 | 1 | |a Akkerman, Onno W |e verfasserin |4 aut | |
| 700 | 1 | |a Hofland, Regina W |e verfasserin |4 aut | |
| 700 | 1 | |a Delemarre, Eveline M |e verfasserin |4 aut | |
| 700 | 1 | |a Nierkens, Stefan |e verfasserin |4 aut | |
| 700 | 1 | |a Verheul, Marije K |e verfasserin |4 aut | |
| 700 | 1 | |a Pollard, Andrew J |e verfasserin |4 aut | |
| 700 | 1 | |a van Dissel, Jaap T |e verfasserin |4 aut | |
| 700 | 1 | |a Ottenhoff, Tom H M |e verfasserin |4 aut | |
| 700 | 1 | |a Trouw, Leendert A |e verfasserin |4 aut | |
| 700 | 1 | |a Joosten, Simone A |e verfasserin |4 aut | |
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