Rapid assessment of changes in phage bioactivity using dynamic light scattering
Extensive efforts are underway to develop bacteriophages as therapies against antibiotic-resistant bacteria. However, these efforts are confounded by the instability of phage preparations and a lack of suitable tools to assess active phage concentrations over time. Here, we use Dynamic Light Scattering (DLS) to measure changes in phage physical state in response to environmental factors and time, finding that phages tend to decay and form aggregates and that the degree of aggregation can be used to predict phage bioactivity. We then use DLS to optimize phage storage conditions for phages from human clinical trials, predict bioactivity in 50-year-old archival stocks, and evaluate phage samples for use in a phage therapy/wound infection model. We also provide a web-application (Phage-ELF) to facilitate DLS studies of phages. We conclude that DLS provides a rapid, convenient, and non-destructive tool for quality control of phage preparations in academic and commercial settings.
| Errataetall: |
UpdateIn: PNAS Nexus. 2023 Nov 27;2(12):pgad406. - PMID 38111822 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2023 |
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| Enthalten in: |
bioRxiv : the preprint server for biology - (2023) vom: 02. Juli |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dharmaraj, Tejas [VerfasserIn] |
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| Links: |
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| Themen: |
Aggregation |
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| Anmerkungen: |
Date Revised 29.12.2023 published: Electronic UpdateIn: PNAS Nexus. 2023 Nov 27;2(12):pgad406. - PMID 38111822 Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1101/2023.07.02.547396 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM359269273 |
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| 520 | |a Extensive efforts are underway to develop bacteriophages as therapies against antibiotic-resistant bacteria. However, these efforts are confounded by the instability of phage preparations and a lack of suitable tools to assess active phage concentrations over time. Here, we use Dynamic Light Scattering (DLS) to measure changes in phage physical state in response to environmental factors and time, finding that phages tend to decay and form aggregates and that the degree of aggregation can be used to predict phage bioactivity. We then use DLS to optimize phage storage conditions for phages from human clinical trials, predict bioactivity in 50-year-old archival stocks, and evaluate phage samples for use in a phage therapy/wound infection model. We also provide a web-application (Phage-ELF) to facilitate DLS studies of phages. We conclude that DLS provides a rapid, convenient, and non-destructive tool for quality control of phage preparations in academic and commercial settings | ||
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| 650 | 4 | |a bacteriophage decay | |
| 650 | 4 | |a bacteriophage therapy | |
| 650 | 4 | |a dynamic light scattering | |
| 700 | 1 | |a Kratochvil, Michael J |e verfasserin |4 aut | |
| 700 | 1 | |a Pourtois, Julie D |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Qingquan |e verfasserin |4 aut | |
| 700 | 1 | |a Hajfathalian, Maryam |e verfasserin |4 aut | |
| 700 | 1 | |a Hargil, Aviv |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Yung-Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Evans, Zoe |e verfasserin |4 aut | |
| 700 | 1 | |a Oromí-Bosch, Agnès |e verfasserin |4 aut | |
| 700 | 1 | |a Berry, Joel D |e verfasserin |4 aut | |
| 700 | 1 | |a McBride, Robert |e verfasserin |4 aut | |
| 700 | 1 | |a Haddock, Naomi L |e verfasserin |4 aut | |
| 700 | 1 | |a Holman, Derek R |e verfasserin |4 aut | |
| 700 | 1 | |a van Belleghem, Jonas D |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Tony H |e verfasserin |4 aut | |
| 700 | 1 | |a Barr, Jeremy J |e verfasserin |4 aut | |
| 700 | 1 | |a Lavigne, Rob |e verfasserin |4 aut | |
| 700 | 1 | |a Heilshorn, Sarah C |e verfasserin |4 aut | |
| 700 | 1 | |a Blankenberg, Francis G |e verfasserin |4 aut | |
| 700 | 1 | |a Bollyky, Paul L |e verfasserin |4 aut | |
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