Development of a bioreactor for in-vitro compression cycling of tissue engineered meniscal implants
© 2023 The Author(s)..
Injuries to the meniscus are common and can impair physical activities. Bioprinted meniscal tissue offers an attractive alternative to donor tissue for meniscal repair but achieving the strength of native tissue is a challenge. Here we report the development of a tissue engineering bioreactor designed to apply repetitive force which may lead to an increase in the compressive modulus and durability of bioprinted meniscal tissues. The modular bioreactor system is composed of a sterilizable tissue culture vessel together with a dock that applies and measures mechanical force. The culture vessel allows for simultaneous compression cycling of two anatomically sized menisci. Using a hybrid linear actuator with a stepper motor, the dock can apply up to 300 N of force at speeds up to 20 mm/s, corresponding to the upper limits of anatomical force and motion in the knee. An interchangeable 22 N load cell was mated between the culture vessel and the dock to log changes in force. Both the culture vessel and dock are maintained in a standard cell culture incubator to provide heat and CO2, while the dock is powered and controlled externally using a step motor drive and customized software.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
|---|---|
| Enthalten in: |
HardwareX - 14(2023) vom: 28. Juni, Seite e00433 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Loverde, Joseph R [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Journal Article |
|---|
| Anmerkungen: |
Date Revised 18.07.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1016/j.ohx.2023.e00433 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM359259693 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM359259693 | ||
| 003 | DE-627 | ||
| 005 | 20231226080509.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ohx.2023.e00433 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1197.xml |
| 035 | |a (DE-627)NLM359259693 | ||
| 035 | |a (NLM)37424929 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Loverde, Joseph R |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Development of a bioreactor for in-vitro compression cycling of tissue engineered meniscal implants |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 18.07.2023 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2023 The Author(s). | ||
| 520 | |a Injuries to the meniscus are common and can impair physical activities. Bioprinted meniscal tissue offers an attractive alternative to donor tissue for meniscal repair but achieving the strength of native tissue is a challenge. Here we report the development of a tissue engineering bioreactor designed to apply repetitive force which may lead to an increase in the compressive modulus and durability of bioprinted meniscal tissues. The modular bioreactor system is composed of a sterilizable tissue culture vessel together with a dock that applies and measures mechanical force. The culture vessel allows for simultaneous compression cycling of two anatomically sized menisci. Using a hybrid linear actuator with a stepper motor, the dock can apply up to 300 N of force at speeds up to 20 mm/s, corresponding to the upper limits of anatomical force and motion in the knee. An interchangeable 22 N load cell was mated between the culture vessel and the dock to log changes in force. Both the culture vessel and dock are maintained in a standard cell culture incubator to provide heat and CO2, while the dock is powered and controlled externally using a step motor drive and customized software | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Knee biomechanics | |
| 650 | 4 | |a Meniscectomy | |
| 650 | 4 | |a Meniscus bioreactor | |
| 650 | 4 | |a Repair | |
| 650 | 4 | |a Tear | |
| 650 | 4 | |a Tissue engineering | |
| 700 | 1 | |a Piroli, Maria |e verfasserin |4 aut | |
| 700 | 1 | |a Klarmann, George J |e verfasserin |4 aut | |
| 700 | 1 | |a Gaston, Joel |e verfasserin |4 aut | |
| 700 | 1 | |a Kenneth Wickiser, J |e verfasserin |4 aut | |
| 700 | 1 | |a Barnhill, Jason |e verfasserin |4 aut | |
| 700 | 1 | |a Gilchrist, Kristin H |e verfasserin |4 aut | |
| 700 | 1 | |a Ho, Vincent B |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t HardwareX |d 2017 |g 14(2023) vom: 28. Juni, Seite e00433 |w (DE-627)NLM278952674 |x 2468-0672 |7 nnns |
| 773 | 1 | 8 | |g volume:14 |g year:2023 |g day:28 |g month:06 |g pages:e00433 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.ohx.2023.e00433 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 14 |j 2023 |b 28 |c 06 |h e00433 | ||