Functional annotation of uncharacterized proteins from Fusobacterium nucleatum : identification of virulence factors
Fusobacterium nucleatum is a gram-negative bacteria associated with diverse infections like appendicitis and colorectal cancer. It mainly attacks the epithelial cells in the oral cavity and throat of the infected individual. It has a single circular genome of 2.7 Mb. Many proteins in F. nucleatum genome are listed as "Uncharacterized." Annotation of these proteins is crucial for obtaining new facts about the pathogen and deciphering the gene regulation, functions, and pathways along with discovery of novel target proteins. In the light of new genomic information, an armoury of bioinformatic tools were used for predicting the physicochemical parameters, domain and motif search, pattern search, and localization of the uncharacterized proteins. The programs such as receiver operating characteristics determine the efficacy of the databases that have been employed for prediction of different parameters at 83.6%. Functions were successfully assigned to 46 uncharacterized proteins which included enzymes, transporter proteins, membrane proteins, binding proteins, etc. Apart from the function prediction, the proteins were also subjected to string analysis to reveal the interacting partners. The annotated proteins were also put through homology-based structure prediction and modeling using Swiss PDB and Phyre2 servers. Two probable virulent factors were also identified which could be investigated further for potential drug-related studies. The assigning of functions to uncharacterized proteins has shown that some of these proteins are important for cell survival inside the host and can act as effective drug targets.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
|---|---|
| Enthalten in: |
Genomics & informatics - 21(2023), 2 vom: 06. Juni, Seite e21 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Rauthan, Kanchan [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Functional annotation |
|---|
| Anmerkungen: |
Date Revised 13.09.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.5808/gi.22065 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM359165605 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM359165605 | ||
| 003 | DE-627 | ||
| 005 | 20231226080309.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.5808/gi.22065 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1197.xml |
| 035 | |a (DE-627)NLM359165605 | ||
| 035 | |a (NLM)37415454 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Rauthan, Kanchan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Functional annotation of uncharacterized proteins from Fusobacterium nucleatum |b identification of virulence factors |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 13.09.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Fusobacterium nucleatum is a gram-negative bacteria associated with diverse infections like appendicitis and colorectal cancer. It mainly attacks the epithelial cells in the oral cavity and throat of the infected individual. It has a single circular genome of 2.7 Mb. Many proteins in F. nucleatum genome are listed as "Uncharacterized." Annotation of these proteins is crucial for obtaining new facts about the pathogen and deciphering the gene regulation, functions, and pathways along with discovery of novel target proteins. In the light of new genomic information, an armoury of bioinformatic tools were used for predicting the physicochemical parameters, domain and motif search, pattern search, and localization of the uncharacterized proteins. The programs such as receiver operating characteristics determine the efficacy of the databases that have been employed for prediction of different parameters at 83.6%. Functions were successfully assigned to 46 uncharacterized proteins which included enzymes, transporter proteins, membrane proteins, binding proteins, etc. Apart from the function prediction, the proteins were also subjected to string analysis to reveal the interacting partners. The annotated proteins were also put through homology-based structure prediction and modeling using Swiss PDB and Phyre2 servers. Two probable virulent factors were also identified which could be investigated further for potential drug-related studies. The assigning of functions to uncharacterized proteins has shown that some of these proteins are important for cell survival inside the host and can act as effective drug targets | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Fusobacterium nucleatum | |
| 650 | 4 | |a functional annotation | |
| 650 | 4 | |a hypothetical proteins | |
| 650 | 4 | |a uncharacterized proteins | |
| 650 | 4 | |a virulence factors | |
| 700 | 1 | |a Joshi, Saranya |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar, Lokesh |e verfasserin |4 aut | |
| 700 | 1 | |a Goel, Divya |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar, Sudhir |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Genomics & informatics |d 2012 |g 21(2023), 2 vom: 06. Juni, Seite e21 |w (DE-627)NLM222180579 |x 1598-866X |7 nnns |
| 773 | 1 | 8 | |g volume:21 |g year:2023 |g number:2 |g day:06 |g month:06 |g pages:e21 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.5808/gi.22065 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 21 |j 2023 |e 2 |b 06 |c 06 |h e21 | ||