Synergistic infection of Edwardsiella ictaluri and Flavobacterium oreochromis in cage cultured tilapia (Oreochromis sp.)
© 2023 John Wiley & Sons Ltd..
Widespread distribution of a highly pathogenic Edwardsiella ictaluri strain in farmed tilapia in northern Vietnam has recently been reported. The subsequent investigation noticed a disease outbreak occurred at five nearby tilapia farms with floating cages, in which the clinical signs of both edwardsiellosis and columnaris diseases were observed on the same infected fish and caused 65% to 85% fish mortality. Naturally diseased fish (n = 109) were sampled from the five infected farms for bacterial identification and conducting challenge tests. The two bacteria Edwardsiella ictaluri and Flavobacterium oreochromis were identified by a combination of biochemical tests, PCR and 16SrRNA sequencing methods. Experimental challenge tests on Nile tilapia resulted in the median lethal dose (LD50 ) of E. ictaluri and F. oreochromis at 70 CFU/fish by intraperitoneal (i.p.) injection and 3.6 × 106 CFU/mL by immersion, respectively. The experimentally co-infected challenged fish exposed to LD50 doses resulted in 83% ± 6% mortality, with the infected fish exhibiting clinical signs of both edwardsiellosis and columnaris diseases, mimicking the naturally diseased fish. This finding suggests that the co-infection of E. ictaluri and F. oreochromis may interact in a synergistic manner, to enhance the overall severity of the infection and elevates the need for efficient methods to control both pathogens.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:46 |
|---|---|
| Enthalten in: |
Journal of fish diseases - 46(2023), 10 vom: 11. Okt., Seite 1125-1136 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Nhinh, Doan Thi [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Co-infection |
|---|
| Anmerkungen: |
Date Completed 14.09.2023 Date Revised 14.09.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/jfd.13832 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM359119964 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM359119964 | ||
| 003 | DE-627 | ||
| 005 | 20231226080215.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/jfd.13832 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1197.xml |
| 035 | |a (DE-627)NLM359119964 | ||
| 035 | |a (NLM)37410863 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Nhinh, Doan Thi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Synergistic infection of Edwardsiella ictaluri and Flavobacterium oreochromis in cage cultured tilapia (Oreochromis sp.) |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 14.09.2023 | ||
| 500 | |a Date Revised 14.09.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 John Wiley & Sons Ltd. | ||
| 520 | |a Widespread distribution of a highly pathogenic Edwardsiella ictaluri strain in farmed tilapia in northern Vietnam has recently been reported. The subsequent investigation noticed a disease outbreak occurred at five nearby tilapia farms with floating cages, in which the clinical signs of both edwardsiellosis and columnaris diseases were observed on the same infected fish and caused 65% to 85% fish mortality. Naturally diseased fish (n = 109) were sampled from the five infected farms for bacterial identification and conducting challenge tests. The two bacteria Edwardsiella ictaluri and Flavobacterium oreochromis were identified by a combination of biochemical tests, PCR and 16SrRNA sequencing methods. Experimental challenge tests on Nile tilapia resulted in the median lethal dose (LD50 ) of E. ictaluri and F. oreochromis at 70 CFU/fish by intraperitoneal (i.p.) injection and 3.6 × 106 CFU/mL by immersion, respectively. The experimentally co-infected challenged fish exposed to LD50 doses resulted in 83% ± 6% mortality, with the infected fish exhibiting clinical signs of both edwardsiellosis and columnaris diseases, mimicking the naturally diseased fish. This finding suggests that the co-infection of E. ictaluri and F. oreochromis may interact in a synergistic manner, to enhance the overall severity of the infection and elevates the need for efficient methods to control both pathogens | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Edwardsiella ictaluri | |
| 650 | 4 | |a Flavobacterium oreochromis | |
| 650 | 4 | |a co-infection | |
| 650 | 4 | |a disease outbreak | |
| 650 | 4 | |a tilapia | |
| 700 | 1 | |a Hoa, Dang Thi |e verfasserin |4 aut | |
| 700 | 1 | |a Giang, Nguyen Thi Huong |e verfasserin |4 aut | |
| 700 | 1 | |a Van Van, Kim |e verfasserin |4 aut | |
| 700 | 1 | |a Dang, Lua Thi |e verfasserin |4 aut | |
| 700 | 1 | |a Crumlish, Mags |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Ha Thanh |e verfasserin |4 aut | |
| 700 | 1 | |a Hoai, Truong Dinh |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of fish diseases |d 1998 |g 46(2023), 10 vom: 11. Okt., Seite 1125-1136 |w (DE-627)NLM098166034 |x 1365-2761 |7 nnns |
| 773 | 1 | 8 | |g volume:46 |g year:2023 |g number:10 |g day:11 |g month:10 |g pages:1125-1136 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/jfd.13832 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 46 |j 2023 |e 10 |b 11 |c 10 |h 1125-1136 | ||