Whole-genome sequencing reveals widespread presence of Staphylococcus capitis NRCS-A clone in neonatal units across the United Kingdom
Crown Copyright © 2023. Published by Elsevier Ltd. All rights reserved..
OBJECTIVE: Increased incidence of neonatal Staphylococcus capitis bacteraemia in summer 2020, London, raised suspicion of widespread multidrug-resistant clone NRCS-A. We set out to investigate the molecular epidemiology of this clone in neonatal units (NNUs) across the UK.
METHODS: We conducted whole-genome sequencing (WGS) on presumptive S. capitis NRCS-A isolates collected from infants admitted to nationwide NNUs and from environmental sampling in two distinct NNUs in 2021. Previously published S. capitis genomes were added for comparison. Genetic clusters of NRCS-A isolates were defined based on core-genome single-nucleotide polymorphisms.
RESULTS: We analysed WGS data of 838 S. capitis isolates and identified 750 NRCS-A isolates. We discovered a possible UK-specific NRCS-A lineage consisting of 611 isolates collected between 2005 and 2021. We determined 28 genetic clusters of NRCS-A isolates, which covered all geographical regions in the UK, and isolates of 19 genetic clusters were found in ≥2 regions, suggesting inter-regional spread. Within the NRCS-A clone, strong genetic relatedness was identified between contemporary clinical and incubator-associated fomite isolates and between clinical isolates associated with inter-hospital infant transfer.
CONCLUSIONS: This WGS-based study confirms the dispersion of S. capitis NRCS-A clone amongst NNUs across the UK and urges research on improving clinical management of neonatal S. capitis infection.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:87 |
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| Enthalten in: |
The Journal of infection - 87(2023), 3 vom: 30. Sept., Seite 210-219 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wan, Yu [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 09.08.2023 Date Revised 09.08.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jinf.2023.06.020 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM358952417 |
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| 245 | 1 | 0 | |a Whole-genome sequencing reveals widespread presence of Staphylococcus capitis NRCS-A clone in neonatal units across the United Kingdom |
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| 500 | |a Date Revised 09.08.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Crown Copyright © 2023. Published by Elsevier Ltd. All rights reserved. | ||
| 520 | |a OBJECTIVE: Increased incidence of neonatal Staphylococcus capitis bacteraemia in summer 2020, London, raised suspicion of widespread multidrug-resistant clone NRCS-A. We set out to investigate the molecular epidemiology of this clone in neonatal units (NNUs) across the UK | ||
| 520 | |a METHODS: We conducted whole-genome sequencing (WGS) on presumptive S. capitis NRCS-A isolates collected from infants admitted to nationwide NNUs and from environmental sampling in two distinct NNUs in 2021. Previously published S. capitis genomes were added for comparison. Genetic clusters of NRCS-A isolates were defined based on core-genome single-nucleotide polymorphisms | ||
| 520 | |a RESULTS: We analysed WGS data of 838 S. capitis isolates and identified 750 NRCS-A isolates. We discovered a possible UK-specific NRCS-A lineage consisting of 611 isolates collected between 2005 and 2021. We determined 28 genetic clusters of NRCS-A isolates, which covered all geographical regions in the UK, and isolates of 19 genetic clusters were found in ≥2 regions, suggesting inter-regional spread. Within the NRCS-A clone, strong genetic relatedness was identified between contemporary clinical and incubator-associated fomite isolates and between clinical isolates associated with inter-hospital infant transfer | ||
| 520 | |a CONCLUSIONS: This WGS-based study confirms the dispersion of S. capitis NRCS-A clone amongst NNUs across the UK and urges research on improving clinical management of neonatal S. capitis infection | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Bacteraemia | |
| 650 | 4 | |a Molecular epidemiology | |
| 650 | 4 | |a NRCS-A clone | |
| 650 | 4 | |a Neonatal units | |
| 650 | 4 | |a Phylogenetics | |
| 650 | 4 | |a Population structure | |
| 650 | 4 | |a Staphylococcus capitis | |
| 650 | 4 | |a Whole-genome sequencing | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 700 | 1 | |a Ganner, Mark |e verfasserin |4 aut | |
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| 700 | 1 | |a Ready, Derren |e verfasserin |4 aut | |
| 700 | 1 | |a Moore, Ginny |e verfasserin |4 aut | |
| 700 | 1 | |a Potterill, Isabelle |e verfasserin |4 aut | |
| 700 | 1 | |a Paranthaman, Karthik |e verfasserin |4 aut | |
| 700 | 1 | |a Jauneikaite, Elita |e verfasserin |4 aut | |
| 700 | 1 | |a Patel, Bharat |e verfasserin |4 aut | |
| 700 | 1 | |a Harley, Alessandra |e verfasserin |4 aut | |
| 700 | 1 | |a Getino, Maria |e verfasserin |4 aut | |
| 700 | 0 | |a UKHSA S. capitis Incident Management Team |e verfasserin |4 aut | |
| 700 | 1 | |a Brown, Colin S |e verfasserin |4 aut | |
| 700 | 1 | |a Demirjian, Alicia |e verfasserin |4 aut | |
| 700 | 1 | |a Pichon, Bruno |e verfasserin |4 aut | |
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