A simple strategy based on ATR-FTIR difference spectroscopy to monitor substrate intake and metabolite release by growing bacteria
Copyright © 2023 Elsevier B.V. All rights reserved..
Attenuated total reflectance Fourier transform infrared (ATR-FTIR) difference spectroscopy has been employed for a variety of applications spanning from reaction mechanisms analysis to interface phenomena assessment. This technique is based on the detection of spectral changes induced by the chemical modification of the original sample. In the present study, we highlight the potential of the ATR-FTIR difference approach in the field of microbial biochemistry and biotechnology, reporting on the identification of main soluble species consumed and released by growing bacteria during the biohydrogen production process. Specifically, the mid-infrared spectrum of a model culture broth, composed of glucose, malt extract and yeast extract, was used as background to acquire the FTIR difference spectrum of the same broth as modified by Enterobacter aerogenes metabolism. The analysis of difference signals revealed that only glucose is degraded during hydrogen evolution in anaerobic conditions, while ethanol and 2,3-butanediol are the main soluble metabolites released with H2. This fast and easy analytical approach can therefore represent a sustainable strategy to screen different bacterial strains and to select raw and waste materials to be employed in the field of biofuel production.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:302 |
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| Enthalten in: |
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy - 302(2023) vom: 05. Dez., Seite 123031 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Semeraro, Paola [VerfasserIn] |
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| Links: |
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| Themen: |
Attenuated total reflectance |
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| Anmerkungen: |
Date Completed 07.09.2023 Date Revised 07.09.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.saa.2023.123031 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM358937787 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a Attenuated total reflectance Fourier transform infrared (ATR-FTIR) difference spectroscopy has been employed for a variety of applications spanning from reaction mechanisms analysis to interface phenomena assessment. This technique is based on the detection of spectral changes induced by the chemical modification of the original sample. In the present study, we highlight the potential of the ATR-FTIR difference approach in the field of microbial biochemistry and biotechnology, reporting on the identification of main soluble species consumed and released by growing bacteria during the biohydrogen production process. Specifically, the mid-infrared spectrum of a model culture broth, composed of glucose, malt extract and yeast extract, was used as background to acquire the FTIR difference spectrum of the same broth as modified by Enterobacter aerogenes metabolism. The analysis of difference signals revealed that only glucose is degraded during hydrogen evolution in anaerobic conditions, while ethanol and 2,3-butanediol are the main soluble metabolites released with H2. This fast and easy analytical approach can therefore represent a sustainable strategy to screen different bacterial strains and to select raw and waste materials to be employed in the field of biofuel production | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Attenuated total reflectance | |
| 650 | 4 | |a Biohydrogen | |
| 650 | 4 | |a Culture broth analysis | |
| 650 | 4 | |a Dark fermentation | |
| 650 | 4 | |a Enterobacter aerogenes | |
| 650 | 4 | |a Infrared spectroscopy | |
| 650 | 7 | |a Biofuels |2 NLM | |
| 700 | 1 | |a Giotta, Livia |e verfasserin |4 aut | |
| 700 | 1 | |a Talà, Adelfia |e verfasserin |4 aut | |
| 700 | 1 | |a Tufariello, Maria |e verfasserin |4 aut | |
| 700 | 1 | |a D'Elia, Marcella |e verfasserin |4 aut | |
| 700 | 1 | |a Milano, Francesco |e verfasserin |4 aut | |
| 700 | 1 | |a Alifano, Pietro |e verfasserin |4 aut | |
| 700 | 1 | |a Valli, Ludovico |e verfasserin |4 aut | |
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