Mutations in cdon and boc affect trunk neural crest cell migration and slow-twitch muscle development in zebrafish
© 2023. Published by The Company of Biologists Ltd..
The transmembrane proteins cdon and boc are implicated in regulating hedgehog signaling during vertebrate development. Recent work showing roles for these genes in axon guidance and neural crest cell migration suggest that cdon and boc may play additional functions in regulating directed cell movements. We use newly generated and existing mutants to investigate a role for cdon and boc in zebrafish neural crest cell migration. We find that single mutant embryos exhibit normal neural crest phenotypes, but that neural crest migration is strikingly disrupted in double cdon;boc mutant embryos. We further show that this migration phenotype is associated with defects in the differentiation of slow-twitch muscle cells, and the loss of a Col1a1a-containing extracellular matrix, suggesting that neural crest defects may be a secondary consequence to defects in mesoderm development. Combined, our data add to a growing literature showing that cdon and boc act synergistically to promote hedgehog signaling during vertebrate development, and suggest that the zebrafish can be used to study the function of hedgehog receptor paralogs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:150 |
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| Enthalten in: |
Development (Cambridge, England) - 150(2023), 14 vom: 15. Juli |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lencer, Ezra [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 20.07.2023 Date Revised 22.07.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1242/dev.201304 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM358914795 |
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| 100 | 1 | |a Lencer, Ezra |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Mutations in cdon and boc affect trunk neural crest cell migration and slow-twitch muscle development in zebrafish |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Revised 22.07.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. Published by The Company of Biologists Ltd. | ||
| 520 | |a The transmembrane proteins cdon and boc are implicated in regulating hedgehog signaling during vertebrate development. Recent work showing roles for these genes in axon guidance and neural crest cell migration suggest that cdon and boc may play additional functions in regulating directed cell movements. We use newly generated and existing mutants to investigate a role for cdon and boc in zebrafish neural crest cell migration. We find that single mutant embryos exhibit normal neural crest phenotypes, but that neural crest migration is strikingly disrupted in double cdon;boc mutant embryos. We further show that this migration phenotype is associated with defects in the differentiation of slow-twitch muscle cells, and the loss of a Col1a1a-containing extracellular matrix, suggesting that neural crest defects may be a secondary consequence to defects in mesoderm development. Combined, our data add to a growing literature showing that cdon and boc act synergistically to promote hedgehog signaling during vertebrate development, and suggest that the zebrafish can be used to study the function of hedgehog receptor paralogs | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Adaxial cell | |
| 650 | 4 | |a Cell guidance | |
| 650 | 4 | |a Cell migration | |
| 650 | 4 | |a Extracellular matrix | |
| 650 | 4 | |a Hedgehog signaling | |
| 650 | 4 | |a Neural crest cell | |
| 650 | 7 | |a Cell Adhesion Molecules |2 NLM | |
| 650 | 7 | |a Hedgehog Proteins |2 NLM | |
| 650 | 7 | |a BOC protein, zebrafish |2 NLM | |
| 650 | 7 | |a Cdon protein, zebrafish |2 NLM | |
| 700 | 1 | |a Rains, Addison |e verfasserin |4 aut | |
| 700 | 1 | |a Binne, Erin |e verfasserin |4 aut | |
| 700 | 1 | |a Prekeris, Rytis |e verfasserin |4 aut | |
| 700 | 1 | |a Artinger, Kristin B |e verfasserin |4 aut | |
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