Spatially resolved single-cell translatomics at molecular resolution
The precise control of messenger RNA (mRNA) translation is a crucial step in posttranscriptional gene regulation of cellular physiology. However, it remains a challenge to systematically study mRNA translation at the transcriptomic scale with spatial and single-cell resolution. Here, we report the development of ribosome-bound mRNA mapping (RIBOmap), a highly multiplexed three-dimensional in situ profiling method to detect cellular translatome. RIBOmap profiling of 981 genes in HeLa cells revealed cell cycle-dependent translational control and colocalized translation of functional gene modules. We mapped 5413 genes in mouse brain tissues, yielding spatially resolved single-cell translatomic profiles for 119,173 cells and revealing cell type-specific and brain region-specific translational regulation, including translation remodeling during oligodendrocyte maturation. Our method detected widespread patterns of localized translation in neuronal and glial cells in intact brain tissue networks.
| Errataetall: |
ErratumIn: Science. 2024 Mar 8;383(6687):eado9025. - PMID 38452096 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:380 |
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| Enthalten in: |
Science (New York, N.Y.) - 380(2023), 6652 vom: 30. Juni, Seite eadd3067 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zeng, Hu [VerfasserIn] |
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| Anmerkungen: |
Date Completed 11.07.2023 Date Revised 07.03.2024 published: Print-Electronic ErratumIn: Science. 2024 Mar 8;383(6687):eado9025. - PMID 38452096 Citation Status MEDLINE |
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| doi: |
10.1126/science.add3067 |
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| 520 | |a The precise control of messenger RNA (mRNA) translation is a crucial step in posttranscriptional gene regulation of cellular physiology. However, it remains a challenge to systematically study mRNA translation at the transcriptomic scale with spatial and single-cell resolution. Here, we report the development of ribosome-bound mRNA mapping (RIBOmap), a highly multiplexed three-dimensional in situ profiling method to detect cellular translatome. RIBOmap profiling of 981 genes in HeLa cells revealed cell cycle-dependent translational control and colocalized translation of functional gene modules. We mapped 5413 genes in mouse brain tissues, yielding spatially resolved single-cell translatomic profiles for 119,173 cells and revealing cell type-specific and brain region-specific translational regulation, including translation remodeling during oligodendrocyte maturation. Our method detected widespread patterns of localized translation in neuronal and glial cells in intact brain tissue networks | ||
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| 700 | 1 | |a Ren, Jingyi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Connie Kangni |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Zefang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Haowen |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Yiming |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Hailing |e verfasserin |4 aut | |
| 700 | 1 | |a Aditham, Abhishek |e verfasserin |4 aut | |
| 700 | 1 | |a Sui, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Hongyu |e verfasserin |4 aut | |
| 700 | 1 | |a Lo, Jennifer A |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiao |e verfasserin |4 aut | |
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