The Mucin-Like Domain of the Ebola Glycoprotein Does Not Impact Virulence or Pathogenicity in Ferrets
© The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
BACKGROUND: Ebola virus (EBOV) is considered among the most dangerous viruses with case fatality rates approaching 90% depending on the outbreak. While several viral proteins (VPs) including VP24, VP35, and the soluble glycoprotein are understood to contribute to virulence, less is known of the contribution of the highly variable mucin-like domain (MLD) of EBOV. Early studies have defined a potential role in immune evasion of the MLD by providing a glycan shield to critical glycoprotein residues tied to viral entry. Nonetheless, little is known as to what direct role the MLD plays in acute EBOV disease (EVD).
METHODS: We generated an infectious EBOV clone that lacks the MLD and assessed its virulence in ferrets compared with wild-type (WT) virus.
RESULTS: No differences in growth kinetics were observed in vitro, nor were there any differences in time to death, viremia, or clinical picture in ferrets infected with recombinant EBOV (rEBOV)-WT or rEBOV-Δmucin.
CONCLUSIONS: The EBOV MLD does not play a critical role in acute pathogenesis of EVD in ferrets.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:228 |
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| Enthalten in: |
The Journal of infectious diseases - 228(2023), Suppl 7 vom: 15. Nov., Seite S587-S593 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Halfmann, Peter J [VerfasserIn] |
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| Links: |
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| Themen: |
Ebola virus |
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| Anmerkungen: |
Date Completed 14.02.2024 Date Revised 14.02.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/infdis/jiad240 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM35880874X |
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| 100 | 1 | |a Halfmann, Peter J |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The Mucin-Like Domain of the Ebola Glycoprotein Does Not Impact Virulence or Pathogenicity in Ferrets |
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| 500 | |a Date Revised 14.02.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a BACKGROUND: Ebola virus (EBOV) is considered among the most dangerous viruses with case fatality rates approaching 90% depending on the outbreak. While several viral proteins (VPs) including VP24, VP35, and the soluble glycoprotein are understood to contribute to virulence, less is known of the contribution of the highly variable mucin-like domain (MLD) of EBOV. Early studies have defined a potential role in immune evasion of the MLD by providing a glycan shield to critical glycoprotein residues tied to viral entry. Nonetheless, little is known as to what direct role the MLD plays in acute EBOV disease (EVD) | ||
| 520 | |a METHODS: We generated an infectious EBOV clone that lacks the MLD and assessed its virulence in ferrets compared with wild-type (WT) virus | ||
| 520 | |a RESULTS: No differences in growth kinetics were observed in vitro, nor were there any differences in time to death, viremia, or clinical picture in ferrets infected with recombinant EBOV (rEBOV)-WT or rEBOV-Δmucin | ||
| 520 | |a CONCLUSIONS: The EBOV MLD does not play a critical role in acute pathogenesis of EVD in ferrets | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Ebola virus | |
| 650 | 4 | |a ferrets | |
| 650 | 4 | |a mucin-like domain | |
| 650 | 4 | |a reverse genetics | |
| 650 | 7 | |a Mucins |2 NLM | |
| 650 | 7 | |a Glycoproteins |2 NLM | |
| 700 | 1 | |a Borisevich, Viktoriya |e verfasserin |4 aut | |
| 700 | 1 | |a Levine, Corri B |e verfasserin |4 aut | |
| 700 | 1 | |a Mire, Chad E |e verfasserin |4 aut | |
| 700 | 1 | |a Fenton, Karla A |e verfasserin |4 aut | |
| 700 | 1 | |a Geisbert, Thomas W |e verfasserin |4 aut | |
| 700 | 1 | |a Kawaoka, Yoshihiro |e verfasserin |4 aut | |
| 700 | 1 | |a Cross, Robert W |e verfasserin |4 aut | |
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