The colonic metabolism differences of main alkaloids in normal and colitis mice treated with Coptis chinensis Franch. and Sophora flavescens Ait. herbal pair using liquid chromatography-high resolution mass spectrometry method combined with chemometrics
© 2023 Wiley-VCH GmbH..
Coptis chinensis Franch. and Sophora flavescens Ait. is a herbal pair frequently used in treating ulcerative colitis. However, the bio-disposition profile of the major components in the inflamed gut remains unclear, which is essential to understand the pharmacological material basis of this herb pair. Here we established an integral quantitative and chemometric method to deduce the colonic metabolism differences of this herbal pair in normal and colitis mice. With this LC-MS method, a total of 41 components have been found in the Coptis chinensis Franch. and Sophora flavescens Ait. extract, and 28 metabolites were found in the colon after oral administration. Alkaloid and its phase I metabolites were the main components in the colon of normal and colitis mice. The results of principal component analysis at 6 h after oral administration showed significant colonic metabolism differences between normal and colitis mice. Heamap results showed that colitis induced significant changes in the colonic bio-disposition of this herbal pair extract. In particular, in the context of colitis, the phase I metabolism of berberine, coptisine, jatrorrhizine, palmatine,and epiberberine has been inhibited. These results may provide a basis for understanding the pharmacological material basis of Coptis chinensis Franch. and Sophora flavescens Ait. in treating ulcerative colitis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:46 |
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| Enthalten in: |
Journal of separation science - 46(2023), 14 vom: 20. Juli, Seite e2300094 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Qin [VerfasserIn] |
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| Links: |
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| Themen: |
Alkaloids |
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| Anmerkungen: |
Date Completed 27.07.2023 Date Revised 27.07.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/jssc.202300094 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM358413044 |
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| 245 | 1 | 4 | |a The colonic metabolism differences of main alkaloids in normal and colitis mice treated with Coptis chinensis Franch. and Sophora flavescens Ait. herbal pair using liquid chromatography-high resolution mass spectrometry method combined with chemometrics |
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| 520 | |a © 2023 Wiley-VCH GmbH. | ||
| 520 | |a Coptis chinensis Franch. and Sophora flavescens Ait. is a herbal pair frequently used in treating ulcerative colitis. However, the bio-disposition profile of the major components in the inflamed gut remains unclear, which is essential to understand the pharmacological material basis of this herb pair. Here we established an integral quantitative and chemometric method to deduce the colonic metabolism differences of this herbal pair in normal and colitis mice. With this LC-MS method, a total of 41 components have been found in the Coptis chinensis Franch. and Sophora flavescens Ait. extract, and 28 metabolites were found in the colon after oral administration. Alkaloid and its phase I metabolites were the main components in the colon of normal and colitis mice. The results of principal component analysis at 6 h after oral administration showed significant colonic metabolism differences between normal and colitis mice. Heamap results showed that colitis induced significant changes in the colonic bio-disposition of this herbal pair extract. In particular, in the context of colitis, the phase I metabolism of berberine, coptisine, jatrorrhizine, palmatine,and epiberberine has been inhibited. These results may provide a basis for understanding the pharmacological material basis of Coptis chinensis Franch. and Sophora flavescens Ait. in treating ulcerative colitis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Coptis chinensis Franch | |
| 650 | 4 | |a Sophora flavescens Ait | |
| 650 | 4 | |a colonic metabolism | |
| 650 | 4 | |a high-resolution mass spectrometry | |
| 650 | 4 | |a ulcerative colitis | |
| 650 | 7 | |a Alkaloids |2 NLM | |
| 650 | 7 | |a Drugs, Chinese Herbal |2 NLM | |
| 700 | 1 | |a Chen, Zihan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jianrong |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Qiuying |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Dongxin |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Liu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jinge |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Cheng |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Jiayi |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Haibo |e verfasserin |4 aut | |
| 700 | 1 | |a Kang, An |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Dongdong |e verfasserin |4 aut | |
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