A protocol for visualization of murine in situ neurovascular interfaces
Published by Elsevier Inc..
Mapping cranial vasculature and adjacent neurovascular interfaces in their entirety will enhance our understanding of central nervous system function in any physiologic state. We present a workflow to visualize in situ murine vasculature and surrounding cranial structures using terminal polymer casting of vessels, iterative sample processing and image acquisition, and automated image registration and processing. While this method does not obtain dynamic imaging due to mouse sacrifice, these studies can be performed before sacrifice and processed with other acquired images. For complete details on the use and execution of this protocol, please refer to Rosenblum et al.1.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:4 |
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| Enthalten in: |
STAR protocols - 4(2023), 3 vom: 15. Sept., Seite 102367 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dang, Danielle D [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Completed 08.03.2024 Date Revised 08.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.xpro.2023.102367 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM358405629 |
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| 245 | 1 | 2 | |a A protocol for visualization of murine in situ neurovascular interfaces |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Published by Elsevier Inc. | ||
| 520 | |a Mapping cranial vasculature and adjacent neurovascular interfaces in their entirety will enhance our understanding of central nervous system function in any physiologic state. We present a workflow to visualize in situ murine vasculature and surrounding cranial structures using terminal polymer casting of vessels, iterative sample processing and image acquisition, and automated image registration and processing. While this method does not obtain dynamic imaging due to mouse sacrifice, these studies can be performed before sacrifice and processed with other acquired images. For complete details on the use and execution of this protocol, please refer to Rosenblum et al.1 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Model Organisms | |
| 650 | 4 | |a Neuroscience | |
| 650 | 4 | |a Systems biology | |
| 700 | 1 | |a Chandrashekhar, Vikram |e verfasserin |4 aut | |
| 700 | 1 | |a Chandrashekhar, Vibhu |e verfasserin |4 aut | |
| 700 | 1 | |a Ghabdanzanluqui, Nagela |e verfasserin |4 aut | |
| 700 | 1 | |a Knutsen, Russell H |e verfasserin |4 aut | |
| 700 | 1 | |a Nazari, Matthew A |e verfasserin |4 aut | |
| 700 | 1 | |a Nimmagadda, Likitha |e verfasserin |4 aut | |
| 700 | 1 | |a Donahue, Danielle R |e verfasserin |4 aut | |
| 700 | 1 | |a McGavern, Dorian B |e verfasserin |4 aut | |
| 700 | 1 | |a Kozel, Beth A |e verfasserin |4 aut | |
| 700 | 1 | |a Heiss, John D |e verfasserin |4 aut | |
| 700 | 1 | |a Pacak, Karel |e verfasserin |4 aut | |
| 700 | 1 | |a Zhuang, Zhengping |e verfasserin |4 aut | |
| 700 | 1 | |a Rosenblum, Jared S |e verfasserin |4 aut | |
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