Extracellular RNAs/TLR3 signaling contributes to acute intestinal injury induced by intestinal ischemia reperfusion in mice
Copyright © 2023. Published by Elsevier B.V..
Toll-like receptor 3 (TLR3), one pattern recognition receptor activated by viral and endogenous RNA, has been recently reported to regulate ischemia/reperfusion (I/R) injury in various organs. However, the role of TLR3 in the development of intestinal I/R injury remains unclear. The aim of this study is to evaluate the effects of extracellular RNAs/TLR3 signaling in intestinal I/R injury. An intestinal I/R injury model was established with superior mesenteric artery occlusion both in wild-type and TLR3 knockout (KO, -/-) mice, and MODE-K cells were subjected to hypoxia/reoxygenation (H/R) to mimic the I/R model in vivo. Extracellular RNAs (exRNAs), especially double-stranded RNAs (dsRNAs) co-localized with TLR3, were significantly increased both in vitro and in vivo. Compared with wild-type mice, TLR3 knockout obviously attenuated intestinal I/R injury. Both TLR3/dsRNA complex inhibitor and TLR3 siRNA administration reduced TLR3 expressions and subsequently inhibited intestinal inflammatory cytokine production and apoptosis. In conclusion, exRNAs/TLR3 signaling is a key mechanism that regulates intestinal I/R injury in adult mice, and the TLR3/dsRNA complex inhibitor can be an effective approach for attenuating intestinal I/R-induced inflammatory response and apoptosis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1869 |
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| Enthalten in: |
Biochimica et biophysica acta. Molecular basis of disease - 1869(2023), 7 vom: 01. Okt., Seite 166790 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lei, Yu-Qiong [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.08.2023 Date Revised 07.08.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bbadis.2023.166790 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM358379059 |
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| 100 | 1 | |a Lei, Yu-Qiong |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Extracellular RNAs/TLR3 signaling contributes to acute intestinal injury induced by intestinal ischemia reperfusion in mice |
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| 520 | |a Copyright © 2023. Published by Elsevier B.V. | ||
| 520 | |a Toll-like receptor 3 (TLR3), one pattern recognition receptor activated by viral and endogenous RNA, has been recently reported to regulate ischemia/reperfusion (I/R) injury in various organs. However, the role of TLR3 in the development of intestinal I/R injury remains unclear. The aim of this study is to evaluate the effects of extracellular RNAs/TLR3 signaling in intestinal I/R injury. An intestinal I/R injury model was established with superior mesenteric artery occlusion both in wild-type and TLR3 knockout (KO, -/-) mice, and MODE-K cells were subjected to hypoxia/reoxygenation (H/R) to mimic the I/R model in vivo. Extracellular RNAs (exRNAs), especially double-stranded RNAs (dsRNAs) co-localized with TLR3, were significantly increased both in vitro and in vivo. Compared with wild-type mice, TLR3 knockout obviously attenuated intestinal I/R injury. Both TLR3/dsRNA complex inhibitor and TLR3 siRNA administration reduced TLR3 expressions and subsequently inhibited intestinal inflammatory cytokine production and apoptosis. In conclusion, exRNAs/TLR3 signaling is a key mechanism that regulates intestinal I/R injury in adult mice, and the TLR3/dsRNA complex inhibitor can be an effective approach for attenuating intestinal I/R-induced inflammatory response and apoptosis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a Double-stranded RNAs | |
| 650 | 4 | |a Extracellular RNAs | |
| 650 | 4 | |a Inflammation | |
| 650 | 4 | |a Intestine | |
| 650 | 4 | |a Ischemia/reperfusion | |
| 650 | 4 | |a Toll-like receptor 3 | |
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| 700 | 1 | |a Wan, Yan-Tong |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Guang-Tao |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Yu-Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Si-Dan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wen-Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Wei-Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Ke-Xuan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xi-Yang |e verfasserin |4 aut | |
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